Streptomycin interference in Jaffe reaction - Possible false positive creatinine estimation in excessive dose exposure

Objectives: To study the potential of commonly used aminoglycoside antibiotics to form non-creatinine chromogen with alkaline picrate reagent. Design and methods: We studied the non-creatinine chromogen formation of various concentrations of streptomycin, amikacin, kanamycin, netilmicin, gentamicin and tobramycin added to known creatinine concentrations by the Jaffe reaction based creatinine estimation. Results: Only streptomycin above therapeutic concentrations of 10. mg/mL interfered in the Jaffe reaction and acted as non-creatinine chromogen. Conclusions: Therapeutic doses of the aminoglycosides do not form non-creatinine chromogens.