Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Malaria Journal |
| Vol/bind | 2 |
| Sider (fra-til) | 27 |
| ISSN | 1475-2875 |
| DOI | |
| Status | Udgivet - 2003 |
Bibliografisk note
Keywords: Animals; Antigens, Protozoan; Chromosome Mapping; Cloning, Molecular; DNA, Intergenic; Erythrocyte Membrane; Exons; Introns; Membrane Proteins; Multigene Family; Plasmodium falciparum; Protein Structure, Tertiary; Protozoan Proteins; Pseudogenes; Sequence Analysis, DNA; Variation (Genetics)Adgang til dokumentet
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Sub-grouping of Plasmodium falciparum 3D7 var genes based on sequence analysis of coding and non-coding regions. / Lavstsen, Thomas; Salanti, Ali; Jensen, Anja T R; Arnot, David E; Theander, Thor G.
I: Malaria Journal, Bind 2, 2003, s. 27.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Sub-grouping of Plasmodium falciparum 3D7 var genes based on sequence analysis of coding and non-coding regions
AU - Lavstsen, Thomas
AU - Salanti, Ali
AU - Jensen, Anja T R
AU - Arnot, David E
AU - Theander, Thor G
N1 - Keywords: Animals; Antigens, Protozoan; Chromosome Mapping; Cloning, Molecular; DNA, Intergenic; Erythrocyte Membrane; Exons; Introns; Membrane Proteins; Multigene Family; Plasmodium falciparum; Protein Structure, Tertiary; Protozoan Proteins; Pseudogenes; Sequence Analysis, DNA; Variation (Genetics)
PY - 2003
Y1 - 2003
N2 - BACKGROUND: The variant surface antigen family Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1) is an important target for protective immunity and is implicated in the pathology of malaria through its ability to adhere to host endothelial receptors. The sequence diversity and organization of the 3D7 PfEMP1 repertoire was investigated on the basis of the complete genome sequence. METHODS: Using two tree-building methods we analysed the coding and non-coding sequences of 3D7 var and rif genes as well as var genes of other parasite strains. RESULTS: var genes can be sub-grouped into three major groups (group A, B and C) and two intermediate groups B/A and B/C representing transitions between the three major groups. The best defined var group, group A, comprises telomeric genes transcribed towards the telomere encoding PfEMP1s with complex domain structures different from the 4-domain type dominant of groups B and C. Two sequences belonging to the var1 and var2 subfamilies formed independent groups. A rif subgroup transcribed towards the centromere was found neighbouring var genes of group A such that the rif and var 5' regions merged. This organization appeared to be unique for the group A var genes CONCLUSION: The grouping of var genes implies that var gene recombination preferentially occurs within var gene groups and it is speculated that the groups reflect a functional diversification evolved to cope with the varying conditions of transmission and host immune response met by the parasite.
AB - BACKGROUND: The variant surface antigen family Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1) is an important target for protective immunity and is implicated in the pathology of malaria through its ability to adhere to host endothelial receptors. The sequence diversity and organization of the 3D7 PfEMP1 repertoire was investigated on the basis of the complete genome sequence. METHODS: Using two tree-building methods we analysed the coding and non-coding sequences of 3D7 var and rif genes as well as var genes of other parasite strains. RESULTS: var genes can be sub-grouped into three major groups (group A, B and C) and two intermediate groups B/A and B/C representing transitions between the three major groups. The best defined var group, group A, comprises telomeric genes transcribed towards the telomere encoding PfEMP1s with complex domain structures different from the 4-domain type dominant of groups B and C. Two sequences belonging to the var1 and var2 subfamilies formed independent groups. A rif subgroup transcribed towards the centromere was found neighbouring var genes of group A such that the rif and var 5' regions merged. This organization appeared to be unique for the group A var genes CONCLUSION: The grouping of var genes implies that var gene recombination preferentially occurs within var gene groups and it is speculated that the groups reflect a functional diversification evolved to cope with the varying conditions of transmission and host immune response met by the parasite.
U2 - 10.1186/1475-2875-2-27
DO - 10.1186/1475-2875-2-27
M3 - Journal article
C2 - 14565852
VL - 2
SP - 27
JO - Malaria Journal
JF - Malaria Journal
SN - 1475-2875
ER -