TY - JOUR
T1 - Subretinal saline protects the neuroretina from thermic damage during laser induction of experimental choroidal neovascularization in pigs
AU - Hansen, Silja
AU - Askou, Anne Louise
AU - la Cour, Morten
AU - Corydon, Thomas J.
AU - Bek, Toke
N1 - Funding Information:
Supported by Aarhus University, Fight for Sight Denmark, Synoptikfonden, Maskinfabrikant Jochum Jensen og hustru Mette Marie Jensen?s Foundation, and K?bmand Marie Kirstine Jensen?s Foundation. Vitreo-retinal surgeon Jesper Eriksen is thanked for valuable advice on the surgical procedures and professor Jesper Hjortdahl for lending surgical equipment. The AU Health Bioimaging Core Facility is thanked for the use of equipment and technical assistance. The animal facility, Department of Clinical Medicine, Aarhus University, is thanked for housing the pigs and providing the operational setting.
Funding Information:
Supported by Aarhus University, Fight for Sight Denmark, Synoptikfonden, Maskinfabrikant Jochum Jensen og hustru Mette Marie Jensen’s Foundation, and Købmand Marie Kirstine Jensen’s Foundation.
Publisher Copyright:
© 2021 The Authors.
PY - 2021/6
Y1 - 2021/6
N2 - Purpose: The purpose of this study was to develop a porcine model for photocoagulation induced choroidal neovascularization (CNV) with high success rate and minimal thermic damage to the neuroretina. Methods: Experimental CNV was induced by laser photocoagulation in both eyes of 16 domestic pigs. In the left eyes, photocoagulation was preceded by subretinal injection of saline to protect the neuroretina from thermic damage, whereas the right eyes were treated with photocoagulation only. The development of the CNV after 3, 7, 14, 28, and 42 days was evaluated by optical coherence tomography (OCT) scanning, fluorescein angiography, and OCT angiography, and by histology after enucleation. Results: From day 7 after the photocoagulation, OCT showed subretinal density in all lesions of 14 alive animals, and either fluorescein or OCT angiography confirmed CNV formation in 11 of 14 of the eyes that had received photocoagulation alone and those in which photocoagulation had been preceded by subretinal injection of saline. In all cases pretreated with subretinal saline, the neuroretina was protected from immediate thermic damage. The formation of CNVs were confirmed by histology. For both groups, the largest lesions were observed within 14 days after photocoagulation. Conclusions: Injection of subretinal saline can protect the retina from thermic damage induced by retinal photocoagulation without reducing the success rate in producing experimental CNV. The effect of interventional studies aimed at reducing photocoagulation induced experimental CNV in pigs can be evaluated within 2 weeks after photocoagulation.
AB - Purpose: The purpose of this study was to develop a porcine model for photocoagulation induced choroidal neovascularization (CNV) with high success rate and minimal thermic damage to the neuroretina. Methods: Experimental CNV was induced by laser photocoagulation in both eyes of 16 domestic pigs. In the left eyes, photocoagulation was preceded by subretinal injection of saline to protect the neuroretina from thermic damage, whereas the right eyes were treated with photocoagulation only. The development of the CNV after 3, 7, 14, 28, and 42 days was evaluated by optical coherence tomography (OCT) scanning, fluorescein angiography, and OCT angiography, and by histology after enucleation. Results: From day 7 after the photocoagulation, OCT showed subretinal density in all lesions of 14 alive animals, and either fluorescein or OCT angiography confirmed CNV formation in 11 of 14 of the eyes that had received photocoagulation alone and those in which photocoagulation had been preceded by subretinal injection of saline. In all cases pretreated with subretinal saline, the neuroretina was protected from immediate thermic damage. The formation of CNVs were confirmed by histology. For both groups, the largest lesions were observed within 14 days after photocoagulation. Conclusions: Injection of subretinal saline can protect the retina from thermic damage induced by retinal photocoagulation without reducing the success rate in producing experimental CNV. The effect of interventional studies aimed at reducing photocoagulation induced experimental CNV in pigs can be evaluated within 2 weeks after photocoagulation.
KW - Experimental choroidal neovascularization
KW - Fluorescein angiography
KW - Porcine model
U2 - 10.1167/TVST.10.7.29
DO - 10.1167/TVST.10.7.29
M3 - Journal article
C2 - 34185056
AN - SCOPUS:85111041737
SN - 2164-2591
VL - 10
JO - Translational Vision Science and Technology
JF - Translational Vision Science and Technology
IS - 7
M1 - 29
ER -