Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Experimental Eye Research |
| Vol/bind | 73 |
| Udgave nummer | 5 |
| Sider (fra-til) | 723-33 |
| Antal sider | 10 |
| ISSN | 0014-4835 |
| DOI | |
| Status | Udgivet - 2001 |
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(C) 2001 Academic Press.Adgang til dokumentet
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I: Experimental Eye Research, Bind 73, Nr. 5, 2001, s. 723-33.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Superantigen presentation by human retinal pigment epithelial cells to T cells is dependent on CD2-CD58 and CD18-CD54 molecule interactions.
AU - Jørgensen, A
AU - Junker, N
AU - Kaestel, C G
AU - Liang, Y
AU - Wiencke, A
AU - la Cour, M
AU - Lui, G M
AU - Ødum, N
AU - Nissen, Mogens Holst
AU - Röpke, C
N1 - Keywords: Antibodies, Monoclonal; Antigen Presentation; Antigens, CD; Antigens, CD18; Antigens, CD2; Antigens, CD58; Cell Division; Cell Separation; Cells, Cultured; Flow Cytometry; Humans; Intercellular Adhesion Molecule-1; Interferon Type II; Pigment Epithelium of Eye; Statistics, Nonparametric; Superantigens; T-Lymphocytes
PY - 2001
Y1 - 2001
N2 - Human retinal pigment epithelial (RPE) cells are capable of presenting bacterial superantigens (SAg) to T cells in vitro by ligation of MHC class II molecules on RPE cells with the T cell receptor. The purpose of this study was to evaluate the involvement of adhesion molecules in presentation of SAg. Cultured human fetal and adult RPE cells were treated with interferon-gamma (IFN-gamma, 500 U ml(-1) for 72 hr) and afterwards pulsed with the SAg staphylococcal enterotoxin A (SEA, 500 ng ml(-1) for 2 hr) followed by coculture with freshly obtained T cells isolated from peripheral blood. Proliferation was measured by (3)H-thymidine incorporation assay. In selected experiments, either RPE or T cells were pre-treated with blocking antibodies specific for cell surface molecules. For comparison, dendritic cells were used as superantigen presenting cells for T cells. This study showed that presentation of SEA by RPE cells to resting T cells was dependent on the presence of the molecules CD2, CD58 and CD18, CD54. The cycling status of T cells was decisive, thus resting T cells but not activated T cells were capable to proliferate in response to SEA presentation. Proliferation of T cells induced by adult RPE cells was comparable to the proliferation induced by dendritic cells at concentrations of SAg above 100 ng ml(-1), but at concentrations of SAg below 10 ng ml(-1) the response was significantly lower for SAg presented by RPE cells compared to dendritic cells. The results demonstrate that CD2-CD58 and CD18-CD54 interactions are critical for SAg presentation by RPE cells to T cells. The findings thus suggest that also presentation of peptides to resting T cells by RPE cells may be dependent upon these interactions.
AB - Human retinal pigment epithelial (RPE) cells are capable of presenting bacterial superantigens (SAg) to T cells in vitro by ligation of MHC class II molecules on RPE cells with the T cell receptor. The purpose of this study was to evaluate the involvement of adhesion molecules in presentation of SAg. Cultured human fetal and adult RPE cells were treated with interferon-gamma (IFN-gamma, 500 U ml(-1) for 72 hr) and afterwards pulsed with the SAg staphylococcal enterotoxin A (SEA, 500 ng ml(-1) for 2 hr) followed by coculture with freshly obtained T cells isolated from peripheral blood. Proliferation was measured by (3)H-thymidine incorporation assay. In selected experiments, either RPE or T cells were pre-treated with blocking antibodies specific for cell surface molecules. For comparison, dendritic cells were used as superantigen presenting cells for T cells. This study showed that presentation of SEA by RPE cells to resting T cells was dependent on the presence of the molecules CD2, CD58 and CD18, CD54. The cycling status of T cells was decisive, thus resting T cells but not activated T cells were capable to proliferate in response to SEA presentation. Proliferation of T cells induced by adult RPE cells was comparable to the proliferation induced by dendritic cells at concentrations of SAg above 100 ng ml(-1), but at concentrations of SAg below 10 ng ml(-1) the response was significantly lower for SAg presented by RPE cells compared to dendritic cells. The results demonstrate that CD2-CD58 and CD18-CD54 interactions are critical for SAg presentation by RPE cells to T cells. The findings thus suggest that also presentation of peptides to resting T cells by RPE cells may be dependent upon these interactions.
U2 - 10.1006/exer.2001.1082
DO - 10.1006/exer.2001.1082
M3 - Journal article
C2 - 11747372
SN - 0014-4835
VL - 73
SP - 723
EP - 733
JO - Experimental Eye Research
JF - Experimental Eye Research
IS - 5
ER -