Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries

Faizan Ahmed Sadiq*, Koen De Reu, Mette Burmølle, Sharon Maes, Marc Heyndrickx

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

11 Citationer (Scopus)
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Abstract

Most biofilms within the food industry are formed by multiple bacterial species which co-exist on surfaces as a result of interspecies interactions. These ecological interactions often make these communities tolerant against antimicrobials. Our previous work led to the identification of a large number (327) of highly diverse bacterial species on food contact surfaces of the dairy, meat, and egg industries after routine cleaning and disinfection (C&D) regimes. In the current study, biofilm-forming ability of 92 bacterial strains belonging to 26 genera and 42 species was assessed and synergistic interactions in biofilm formation were investigated by coculturing species in all possible four-species combinations. Out of the total 455 four-species biofilm combinations, greater biofilm mass production, compared to the sum of biofilm masses of individual species in monoculture, was observed in 34 combinations. Around half of the combinations showed synergy in biofilm mass > 1.5-fold and most of the combinations belonged to dairy strains. The highest synergy (3.13-fold) was shown by a combination of dairy strains comprising Stenotrophomonas rhizophila, Bacillus licheniformis, Microbacterium lacticum, and Calidifontibacter indicus. The observed synergy in mixed biofilms turned out to be strain-specific rather than species-dependent. All biofilm combinations showing remarkable synergy appeared to have certain common species in all combinations which shows there are keystone industry-specific bacterial species which stimulate synergy or antagonism and this may have implication for biofilm control in the concerned food industries.
OriginalsprogEngelsk
Artikelnummer1159434
TidsskriftFrontiers in Microbiology
Vol/bind14
Antal sider13
ISSN1664-302X
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
This work was funded under EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions – a program of the European Commission (Grant agreement ID: 101025683). We also acknowledge Villum Foundation, project number 35906 for financial support given to Mette Burmølle. The funding Research Executive Agency (REA), delegated by the European Commission, is not responsible for any use that may be made of the information this article contains.

Publisher Copyright:
Copyright © 2023 Sadiq, De Reu, Burmølle, Maes and Heyndrickx.

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