Synthesis of 2′-amino-LNA: a new strategy

C. Rosenbohm, S.M. Christensen, Daniel Sejer Pedersen, L.-E. Larsen, T. Koch, M.D. Sorensen, J. Wengel

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50 Citationer (Scopus)

Abstract

In this paper we present revised and significantly improved synthetic routes to 2′-amino-LNA (locked nucleic acid). The optimal route is convergent with the synthesis of LNA monomers ("2′-oxy-LNA") via a common intermediate obtained by a mild deacetylation for the liberation of the 2′-hydroxy group to give compound 23 without the concomitant ring closure that affords the 2′-oxy-LNA skeleton. After inversion of the stereochemistry at C2′ and triflate formation at the 2′-hydroxy group a new common intermediate 16 is obtained which gives easy access to a range of other analogues exemplified by the introduction of a sulfur nucleophile leading to the 2′-thio-LNA structure. After substitution of the triflate with azide a basic reduction affords the desired 2′-amino-LNA structure, i.e., compound 18. This new synthesis strategy towards 2′-amino-LNA improves the overall yield significantly and converges the syntheses of 2′-oxy-LNA and LNA analogues.
OriginalsprogEngelsk
TidsskriftOrganic and Biomolecular Chemistry
Vol/bind1
Udgave nummer4
Sider (fra-til)655-663
Antal sider9
ISSN1477-0520
DOI
StatusUdgivet - 2003
Udgivet eksterntJa

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