T-cell dysfunction in HIV-1-infected patients with impaired recovery of CD4 cells despite suppression of viral replication

Christian Erikstrup; Gitte Kronborg; Nicolai Lohse; Sisse Rye Ostrowski; Jan Gerstoft; Henrik Ullum; Christian Erikstrup; Gitte Kronborg; Nicolai Lohse; Sisse Rye Ostrowski; Jan Gerstoft; Henrik Ullum

doi:10.1097/QAI.0b013e3181ca3f7c

T-cell dysfunction in HIV-1-infected patients with impaired recovery of CD4 cells despite suppression of viral replication

Christian Erikstrup, Gitte Kronborg, Nicolai Lohse, Sisse Rye Ostrowski, Jan Gerstoft, Henrik Ullum, Christian Erikstrup, Gitte Kronborg, Nicolai Lohse, Sisse Rye Ostrowski, Jan Gerstoft, Henrik Ullum

Institut for Klinisk Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

35 Citationer (Scopus)

Abstract

Udgivelsesdato: 2010-Mar

Originalsprog	Engelsk
Tidsskrift	J A I D S
Vol/bind	53
Udgave nummer	3
Sider (fra-til)	303-10
Antal sider	8
ISSN	1525-4135
DOI	https://doi.org/10.1097/QAI.0b013e3181ca3f7c https://doi.org/10.1097/QAI.0b013e3181ca3f7c
Status	Udgivet - 1 mar. 2010

Bibliografisk note

Keywords: Adult; Anti-HIV Agents; Antigens, CD28; CD4 Lymphocyte Count; CD4-Positive T-Lymphocytes; Denmark; Female; Flow Cytometry; HIV Infections; HLA-DR Antigens; Humans; Interleukin-10; Interleukin-2; Male; Middle Aged; Viral Load

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Citationsformater

Erikstrup, C., Kronborg, G., Lohse, N., Ostrowski, S. R., Gerstoft, J., Ullum, H., Erikstrup, C., Kronborg, G., Lohse, N., Ostrowski, S. R., Gerstoft, J., & Ullum, H. (2010). T-cell dysfunction in HIV-1-infected patients with impaired recovery of CD4 cells despite suppression of viral replication. J A I D S, 53(3), 303-10. https://doi.org/10.1097/QAI.0b013e3181ca3f7c, https://doi.org/10.1097/QAI.0b013e3181ca3f7c

T-cell dysfunction in HIV-1-infected patients with impaired recovery of CD4 cells despite suppression of viral replication. / Erikstrup, Christian; Kronborg, Gitte; Lohse, Nicolai; Ostrowski, Sisse Rye ; Gerstoft, Jan; Ullum, Henrik; Erikstrup, Christian; Kronborg, Gitte; Lohse, Nicolai; Ostrowski, Sisse Rye ; Gerstoft, Jan; Ullum, Henrik.

I: J A I D S, Bind 53, Nr. 3, 01.03.2010, s. 303-10.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Erikstrup, C, Kronborg, G, Lohse, N, Ostrowski, SR , Gerstoft, J, Ullum, H, Erikstrup, C, Kronborg, G, Lohse, N, Ostrowski, SR , Gerstoft, J & Ullum, H 2010, 'T-cell dysfunction in HIV-1-infected patients with impaired recovery of CD4 cells despite suppression of viral replication', J A I D S, bind 53, nr. 3, s. 303-10. https://doi.org/10.1097/QAI.0b013e3181ca3f7c, https://doi.org/10.1097/QAI.0b013e3181ca3f7c

Erikstrup, Christian ; Kronborg, Gitte ; Lohse, Nicolai ; Ostrowski, Sisse Rye ; Gerstoft, Jan ; Ullum, Henrik ; Erikstrup, Christian ; Kronborg, Gitte ; Lohse, Nicolai ; Ostrowski, Sisse Rye ; Gerstoft, Jan ; Ullum, Henrik. / T-cell dysfunction in HIV-1-infected patients with impaired recovery of CD4 cells despite suppression of viral replication. I: J A I D S. 2010 ; Bind 53, Nr. 3. s. 303-10.

@article{60713fe0aac811df928f000ea68e967b,

title = "T-cell dysfunction in HIV-1-infected patients with impaired recovery of CD4 cells despite suppression of viral replication",

abstract = "INTRODUCTION: CD4 T-cell recovery is impeded in some HIVinfected patients despite successful combination antiretroviral therapy (cART) with suppressed HIV RNA. We hypothesized that T-cell dysfunction would be increased in these patients. METHODS: In the Danish HIV Cohort Study, we identified HIV-1-infected patients initiating cART with a CD4 cell count <100 cells per microliter, followed by HIV RNA <50 copies per milliliter for 3 years. Patients with a CD4 count <200 cells per microliter after 3 years were identified as cases; 42 patients with a CD4 count > or = 200 cells per microliter were selected as controls. Six-color flow cytometry was performed on whole blood. Cytokine levels in supernatants from whole blood stimulations were assessed. RESULTS: The case and control groups comprised 18 and 35 patients, respectively. Cases were older than controls (median: 54/46 years). The fraction of CD28+ cells was decreased among cases in the CD4+ and CD8+ T-cell subsets (P = 0.0014/P = 0.0349) and in the corresponding naive subsets (P = 0.0011/P , 0.0001). Cases had higher expression of human leukocyte antigen (HLA)-DR on naive CD4 and CD8 T cells (P = 0.0007/P = 0.0028). The production of interleukin (IL)-10 and IL-2 to phytohemagglutinin was decreased in cases (P < 0.0001/P = 0.019). CONCLUSIONS: Patients with impaired CD4 recovery shared a dysregulated T-cell phenotype with low CD28, high HLA-DR expression, and low IL-2 and IL-10 production.",

author = "Christian Erikstrup and Gitte Kronborg and Nicolai Lohse and Ostrowski, {Sisse Rye} and Jan Gerstoft and Henrik Ullum and Christian Erikstrup and Gitte Kronborg and Nicolai Lohse and Ostrowski, {Sisse Rye} and Jan Gerstoft and Henrik Ullum",

note = "Keywords: Adult; Anti-HIV Agents; Antigens, CD28; CD4 Lymphocyte Count; CD4-Positive T-Lymphocytes; Denmark; Female; Flow Cytometry; HIV Infections; HLA-DR Antigens; Humans; Interleukin-10; Interleukin-2; Male; Middle Aged; Viral Load",

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doi = "10.1097/QAI.0b013e3181ca3f7c",

language = "English",

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pages = "303--10",

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TY - JOUR

T1 - T-cell dysfunction in HIV-1-infected patients with impaired recovery of CD4 cells despite suppression of viral replication

AU - Erikstrup, Christian

AU - Kronborg, Gitte

AU - Lohse, Nicolai

AU - Ostrowski, Sisse Rye

AU - Gerstoft, Jan

AU - Ullum, Henrik

AU - Erikstrup, Christian

AU - Kronborg, Gitte

AU - Lohse, Nicolai

AU - Ostrowski, Sisse Rye

AU - Gerstoft, Jan

AU - Ullum, Henrik

N1 - Keywords: Adult; Anti-HIV Agents; Antigens, CD28; CD4 Lymphocyte Count; CD4-Positive T-Lymphocytes; Denmark; Female; Flow Cytometry; HIV Infections; HLA-DR Antigens; Humans; Interleukin-10; Interleukin-2; Male; Middle Aged; Viral Load

PY - 2010/3/1

Y1 - 2010/3/1

N2 - INTRODUCTION: CD4 T-cell recovery is impeded in some HIVinfected patients despite successful combination antiretroviral therapy (cART) with suppressed HIV RNA. We hypothesized that T-cell dysfunction would be increased in these patients. METHODS: In the Danish HIV Cohort Study, we identified HIV-1-infected patients initiating cART with a CD4 cell count <100 cells per microliter, followed by HIV RNA <50 copies per milliliter for 3 years. Patients with a CD4 count <200 cells per microliter after 3 years were identified as cases; 42 patients with a CD4 count > or = 200 cells per microliter were selected as controls. Six-color flow cytometry was performed on whole blood. Cytokine levels in supernatants from whole blood stimulations were assessed. RESULTS: The case and control groups comprised 18 and 35 patients, respectively. Cases were older than controls (median: 54/46 years). The fraction of CD28+ cells was decreased among cases in the CD4+ and CD8+ T-cell subsets (P = 0.0014/P = 0.0349) and in the corresponding naive subsets (P = 0.0011/P , 0.0001). Cases had higher expression of human leukocyte antigen (HLA)-DR on naive CD4 and CD8 T cells (P = 0.0007/P = 0.0028). The production of interleukin (IL)-10 and IL-2 to phytohemagglutinin was decreased in cases (P < 0.0001/P = 0.019). CONCLUSIONS: Patients with impaired CD4 recovery shared a dysregulated T-cell phenotype with low CD28, high HLA-DR expression, and low IL-2 and IL-10 production.

AB - INTRODUCTION: CD4 T-cell recovery is impeded in some HIVinfected patients despite successful combination antiretroviral therapy (cART) with suppressed HIV RNA. We hypothesized that T-cell dysfunction would be increased in these patients. METHODS: In the Danish HIV Cohort Study, we identified HIV-1-infected patients initiating cART with a CD4 cell count <100 cells per microliter, followed by HIV RNA <50 copies per milliliter for 3 years. Patients with a CD4 count <200 cells per microliter after 3 years were identified as cases; 42 patients with a CD4 count > or = 200 cells per microliter were selected as controls. Six-color flow cytometry was performed on whole blood. Cytokine levels in supernatants from whole blood stimulations were assessed. RESULTS: The case and control groups comprised 18 and 35 patients, respectively. Cases were older than controls (median: 54/46 years). The fraction of CD28+ cells was decreased among cases in the CD4+ and CD8+ T-cell subsets (P = 0.0014/P = 0.0349) and in the corresponding naive subsets (P = 0.0011/P , 0.0001). Cases had higher expression of human leukocyte antigen (HLA)-DR on naive CD4 and CD8 T cells (P = 0.0007/P = 0.0028). The production of interleukin (IL)-10 and IL-2 to phytohemagglutinin was decreased in cases (P < 0.0001/P = 0.019). CONCLUSIONS: Patients with impaired CD4 recovery shared a dysregulated T-cell phenotype with low CD28, high HLA-DR expression, and low IL-2 and IL-10 production.

U2 - 10.1097/QAI.0b013e3181ca3f7c

DO - 10.1097/QAI.0b013e3181ca3f7c

M3 - Journal article

C2 - 20048679

VL - 53

SP - 303

EP - 310

JO - J A I D S

JF - J A I D S

SN - 1525-4135

IS - 3

ER -