Tet Proteins Connect the O-Linked N-acetylglucosamine Transferase Ogt to Chromatin in Embryonic Stem Cells

Pietro Vella; Andrea Scelfo; Sriganesh Jammula; Fulvio Chiacchiera; Kristine Williams; Alessandro Cuomo; Alessandra Roberto; Jesper Christensen; Tiziana Bonaldi; Kristian Helin; Diego Pasini

doi:10.1016/j.molcel.2012.12.019

Tet Proteins Connect the O-Linked N-acetylglucosamine Transferase Ogt to Chromatin in Embryonic Stem Cells

Pietro Vella, Andrea Scelfo, Sriganesh Jammula, Fulvio Chiacchiera, Kristine Williams, Alessandro Cuomo, Alessandra Roberto, Jesper Christensen, Tiziana Bonaldi, Kristian Helin, Diego Pasini

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

288 Citationer (Scopus)

Abstract

O-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt) activity is essential for embryonic stem cell (ESC) viability and mouse development. Ogt is present both in the cytoplasm and the nucleus of different cell types and catalyzes serine and threonine glycosylation. We have characterized the biochemical features of nuclear Ogt and identified the ten-eleven translocation (TET) proteins Tet1 and Tet2 as stable partners of Ogt in the nucleus of ESCs. We show at a genome-wide level that Ogt preferentially associates with Tet1 to genes promoters in close proximity of CpG-rich transcription start sites. These regions are characterized by low levels of DNA modification, suggesting a link between Tet1 and Ogt activities in regulating CpG island methylation. Finally, we show that Tet1 is required for binding of Ogt to chromatin affecting Tet1 activity. Taken together, our data characterize how O-GlcNAcylation is recruited to chromatin and interacts with the activity of 5-methylcytosine hydroxylases.

Originalsprog	Engelsk
Tidsskrift	Molecular Cell
ISSN	1097-4164
DOI	https://doi.org/10.1016/j.molcel.2012.12.019
Status	Udgivet - 2013

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title = "Tet Proteins Connect the O-Linked N-acetylglucosamine Transferase Ogt to Chromatin in Embryonic Stem Cells",

abstract = "O-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt) activity is essential for embryonic stem cell (ESC) viability and mouse development. Ogt is present both in the cytoplasm and the nucleus of different cell types and catalyzes serine and threonine glycosylation. We have characterized the biochemical features of nuclear Ogt and identified the ten-eleven translocation (TET) proteins Tet1 and Tet2 as stable partners of Ogt in the nucleus of ESCs. We show at a genome-wide level that Ogt preferentially associates with Tet1 to genes promoters in close proximity of CpG-rich transcription start sites. These regions are characterized by low levels of DNA modification, suggesting a link between Tet1 and Ogt activities in regulating CpG island methylation. Finally, we show that Tet1 is required for binding of Ogt to chromatin affecting Tet1 activity. Taken together, our data characterize how O-GlcNAcylation is recruited to chromatin and interacts with the activity of 5-methylcytosine hydroxylases.",

author = "Pietro Vella and Andrea Scelfo and Sriganesh Jammula and Fulvio Chiacchiera and Kristine Williams and Alessandro Cuomo and Alessandra Roberto and Jesper Christensen and Tiziana Bonaldi and Kristian Helin and Diego Pasini",

year = "2013",

doi = "10.1016/j.molcel.2012.12.019",

language = "English",

journal = "Molecular Cell",

issn = "1097-4164",

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TY - JOUR

T1 - Tet Proteins Connect the O-Linked N-acetylglucosamine Transferase Ogt to Chromatin in Embryonic Stem Cells

AU - Vella, Pietro

AU - Scelfo, Andrea

AU - Jammula, Sriganesh

AU - Chiacchiera, Fulvio

AU - Williams, Kristine

AU - Cuomo, Alessandro

AU - Roberto, Alessandra

AU - Christensen, Jesper

AU - Bonaldi, Tiziana

AU - Helin, Kristian

AU - Pasini, Diego

PY - 2013

Y1 - 2013

N2 - O-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt) activity is essential for embryonic stem cell (ESC) viability and mouse development. Ogt is present both in the cytoplasm and the nucleus of different cell types and catalyzes serine and threonine glycosylation. We have characterized the biochemical features of nuclear Ogt and identified the ten-eleven translocation (TET) proteins Tet1 and Tet2 as stable partners of Ogt in the nucleus of ESCs. We show at a genome-wide level that Ogt preferentially associates with Tet1 to genes promoters in close proximity of CpG-rich transcription start sites. These regions are characterized by low levels of DNA modification, suggesting a link between Tet1 and Ogt activities in regulating CpG island methylation. Finally, we show that Tet1 is required for binding of Ogt to chromatin affecting Tet1 activity. Taken together, our data characterize how O-GlcNAcylation is recruited to chromatin and interacts with the activity of 5-methylcytosine hydroxylases.

AB - O-linked N-acetylglucosamine (O-GlcNAc) transferase (Ogt) activity is essential for embryonic stem cell (ESC) viability and mouse development. Ogt is present both in the cytoplasm and the nucleus of different cell types and catalyzes serine and threonine glycosylation. We have characterized the biochemical features of nuclear Ogt and identified the ten-eleven translocation (TET) proteins Tet1 and Tet2 as stable partners of Ogt in the nucleus of ESCs. We show at a genome-wide level that Ogt preferentially associates with Tet1 to genes promoters in close proximity of CpG-rich transcription start sites. These regions are characterized by low levels of DNA modification, suggesting a link between Tet1 and Ogt activities in regulating CpG island methylation. Finally, we show that Tet1 is required for binding of Ogt to chromatin affecting Tet1 activity. Taken together, our data characterize how O-GlcNAcylation is recruited to chromatin and interacts with the activity of 5-methylcytosine hydroxylases.

U2 - 10.1016/j.molcel.2012.12.019

DO - 10.1016/j.molcel.2012.12.019

M3 - Journal article

C2 - 23352454

SN - 1097-4164

JO - Molecular Cell

JF - Molecular Cell

ER -