@article{17857580954411dd86a6000ea68e967b,
title = "The Arabidopsis lue1 mutant defines a katanin p60 ortholog involved in hormonal control of microtubule orientation during cell growth.",
abstract = "The lue1 mutant was previously isolated in a bio-imaging screen for Arabidopsis mutants exhibiting inappropriate regulation of an AtGA20ox1 promoter-luciferase reporter fusion. Here we show that lue1 is allelic to fra2, bot1 and erh3, and encodes a truncated katanin-like microtubule-severing protein (AtKSS). Complementation of lue1 with the wild-type AtKSS gene restored both wild-type stature and luciferase reporter levels. Hormonal responses of lue1 to ethylene and gibberellins revealed inappropriate cortical microtubule reorientation during cell growth. Moreover, a fusion between the AtKSS protein and GFP decorated cortical microtubules. A yeast two-hybrid screen with AtKSS as the bait identified proteins related to those involved in microtubule processing, including a katanin p80 subunit and a kinesin ortholog. These results indicate that AtKSS is involved in microtubule dynamics in response to plant hormones.",
author = "Thomas Bouquin and Ole Mattsson and Henrik Naested and Randy Foster and John Mundy",
note = "Keywords: Adenosine Triphosphatases; Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Cell Division; Ethylenes; Gene Expression Regulation, Plant; Genes, Reporter; Gibberellins; Glucuronidase; Microfibrils; Microscopy, Polarization; Microtubules; Molecular Sequence Data; Mutation; Phenotype; Plant Growth Regulators; Plant Roots; RNA, Messenger; Sequence Homology, Amino Acid",
year = "2003",
language = "English",
volume = "116",
pages = "791--801",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "The/Company of Biologists Ltd.",
number = "Pt 5",
}