The Influence of Low Platelet Count on Whole Blood Aggregometry Assessed by Multiplate

Trine Stissing; Nadia P. Dridi; Sisse R. Ostrowski; Louise Bochsen; Pär I Johansson

doi:10.1177/1076029610397183

The Influence of Low Platelet Count on Whole Blood Aggregometry Assessed by Multiplate

Trine Stissing, Nadia P. Dridi, Sisse R. Ostrowski, Louise Bochsen, Pär I Johansson

Institut for Klinisk Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

93 Citationer (Scopus)

Abstract

The Multiplate, a whole blood (WB) platelet function test, has shown promising results identifying patients on antiplatelet therapy at increased risk of rethrombosis. In the present study, the influence of low platelet count on platelet aggregation was analyzed and compared with aggregation results in an artificial matrix, platelet-rich plasma (PRP). Heparinized and citrated blood was diluted with autologous plasma to platelet concentrations 200 to 25 × 10(9)/L in WB samples (n = 10) and 200 to 100 × 10(9)/L in PRP samples (n = 7). The platelet aggregation was investigated by the ADP-, ASPI-, COL-, and TRAP-test. The WB responses decreased at platelet concentration of ≤100 × 10(9)/L (all P <.03), except for heparin-TRAP (50 × 10(9)/L, P = .008) and citrate-ASPI (150 × 10(9)/L, P = .03). In general, WB samples demonstrated higher aggregation than PRP samples at platelet concentrations 200 to 100 × 10(9)/L (P <.05). In conclusion, platelet concentration of

Originalsprog	Engelsk
Tidsskrift	Clinical and Applied Thrombosis/Hemostasis
Vol/bind	17
Udgave nummer	6
Sider (fra-til)	E211-E217
Antal sider	7
ISSN	1076-0296
DOI	https://doi.org/10.1177/1076029610397183
Status	Udgivet - 2011

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10.1177/1076029610397183

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title = "The Influence of Low Platelet Count on Whole Blood Aggregometry Assessed by Multiplate",

abstract = "The Multiplate, a whole blood (WB) platelet function test, has shown promising results identifying patients on antiplatelet therapy at increased risk of rethrombosis. In the present study, the influence of low platelet count on platelet aggregation was analyzed and compared with aggregation results in an artificial matrix, platelet-rich plasma (PRP). Heparinized and citrated blood was diluted with autologous plasma to platelet concentrations 200 to 25 × 10(9)/L in WB samples (n = 10) and 200 to 100 × 10(9)/L in PRP samples (n = 7). The platelet aggregation was investigated by the ADP-, ASPI-, COL-, and TRAP-test. The WB responses decreased at platelet concentration of ≤100 × 10(9)/L (all P <.03), except for heparin-TRAP (50 × 10(9)/L, P = .008) and citrate-ASPI (150 × 10(9)/L, P = .03). In general, WB samples demonstrated higher aggregation than PRP samples at platelet concentrations 200 to 100 × 10(9)/L (P <.05). In conclusion, platelet concentration of",

author = "Trine Stissing and Dridi, {Nadia P.} and Ostrowski, {Sisse R.} and Louise Bochsen and Johansson, {P{\"a}r I}",

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T1 - The Influence of Low Platelet Count on Whole Blood Aggregometry Assessed by Multiplate

AU - Stissing, Trine

AU - Dridi, Nadia P.

AU - Ostrowski, Sisse R.

AU - Bochsen, Louise

AU - Johansson, Pär I

PY - 2011

Y1 - 2011

N2 - The Multiplate, a whole blood (WB) platelet function test, has shown promising results identifying patients on antiplatelet therapy at increased risk of rethrombosis. In the present study, the influence of low platelet count on platelet aggregation was analyzed and compared with aggregation results in an artificial matrix, platelet-rich plasma (PRP). Heparinized and citrated blood was diluted with autologous plasma to platelet concentrations 200 to 25 × 10(9)/L in WB samples (n = 10) and 200 to 100 × 10(9)/L in PRP samples (n = 7). The platelet aggregation was investigated by the ADP-, ASPI-, COL-, and TRAP-test. The WB responses decreased at platelet concentration of ≤100 × 10(9)/L (all P <.03), except for heparin-TRAP (50 × 10(9)/L, P = .008) and citrate-ASPI (150 × 10(9)/L, P = .03). In general, WB samples demonstrated higher aggregation than PRP samples at platelet concentrations 200 to 100 × 10(9)/L (P <.05). In conclusion, platelet concentration of

AB - The Multiplate, a whole blood (WB) platelet function test, has shown promising results identifying patients on antiplatelet therapy at increased risk of rethrombosis. In the present study, the influence of low platelet count on platelet aggregation was analyzed and compared with aggregation results in an artificial matrix, platelet-rich plasma (PRP). Heparinized and citrated blood was diluted with autologous plasma to platelet concentrations 200 to 25 × 10(9)/L in WB samples (n = 10) and 200 to 100 × 10(9)/L in PRP samples (n = 7). The platelet aggregation was investigated by the ADP-, ASPI-, COL-, and TRAP-test. The WB responses decreased at platelet concentration of ≤100 × 10(9)/L (all P <.03), except for heparin-TRAP (50 × 10(9)/L, P = .008) and citrate-ASPI (150 × 10(9)/L, P = .03). In general, WB samples demonstrated higher aggregation than PRP samples at platelet concentrations 200 to 100 × 10(9)/L (P <.05). In conclusion, platelet concentration of

U2 - 10.1177/1076029610397183

DO - 10.1177/1076029610397183

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JO - Clinical and Applied Thrombosis/Hemostasis

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