Abstract
The first vibrational sum-frequency generation (VSFG) spectra of chondroitin sulfate (CS) interacting with dipalmitoyl phosphatidylcholine (DPPC) at air-liquid interface are reported here, collected at a laser repetition rate of 100 kHz. By studying the VSFG spectra in the regions of 1050-1450 cm−1, 2750-3180 cm−1, and 3200-3825 cm−1, it was concluded that in the presence of Ca2+ions, the head groups together with the head-group-bound water molecules in the DPPC monolayer are strongly influenced by the interaction with CS, while the organization of the phospholipid tails remains mostly unchanged. The interactions were observed at a CS concentration below 200 nM, which exemplifies the potential of VSFG in studying biomolecular interactions at low physiological concentrations. The VSFG spectra recorded in the O-H stretching region at chiral polarization combination imply that CS molecules are organized into ordered macromolecular superstructures with a chiral secondary structure.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Physical Chemistry Chemical Physics |
| Vol/bind | 23 |
| Udgave nummer | 23 |
| Sider (fra-til) | 13389-13395 |
| Antal sider | 7 |
| ISSN | 1463-9076 |
| DOI | |
| Status | Udgivet - 2021 |
Bibliografisk note
Funding Information:This research is funded by the Deutsche Forschungsge-meinschaft (DFG, German Research Foundation) – project GSC 1013 SALSA and project 372486779 – SFB 1340, the Danish National Research Foundation (DNFR107), the Carlsberg Foundation (CF20-0412), and the European Union’s Horizon 2020 Research and Innovation Program under grant number 899687 (HS-SEQ). Z. H. acknowledges the funding of her Julia Lermon-tova Fellowship by GSC 1013 SALSA and S. K. is grateful for the support of the German Academic Exchange Service (DAAD). The authors are grateful to Prof. Janina Kneipp for her support of the SALSA Photonics Lab.
Funding Information:
This research is funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - project GSC 1013 SALSA and project 372486779 - SFB 1340, the Danish National Research Foundation (DNFR107), the Carlsberg Foundation (CF20-0412), and the European Union's Horizon 2020 Research and Innovation Program under grant number 899687 (HS-SEQ). Z. H. acknowledges the funding of her Julia Lermontova Fellowship by GSC 1013 SALSA and S. K. is grateful for the support of the German Academic Exchange Service (DAAD). The authors are grateful to Prof. Janina Kneipp for her support of the SALSA Photonics Lab.
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