The last Viking King: a royal maternity case solved by ancient DNA analysis

Jørgen Dissing; Jonas Binladen; Anders Hansen; Birgitte Sejrsen; Eske Willerslev; Niels Lynnerup

doi:10.1016/j.forsciint.2006.03.020

The last Viking King: a royal maternity case solved by ancient DNA analysis

Jørgen Dissing, Jonas Binladen, Anders Hansen, Birgitte Sejrsen, Eske Willerslev, Niels Lynnerup

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

21 Citationer (Scopus)

Abstract

Udgivelsesdato: 2007-Feb-14

Originalsprog	Engelsk
Tidsskrift	Forensic Science International
Vol/bind	166
Udgave nummer	1
Sider (fra-til)	21-7
Antal sider	7
ISSN	0379-0738
DOI	https://doi.org/10.1016/j.forsciint.2006.03.020
Status	Udgivet - 2006

Bibliografisk note

Keywords: Base Sequence; DNA, Mitochondrial; Denmark; European Continental Ancestry Group; Female; Forensic Medicine; History, Ancient; Humans; Male; Molecular Sequence Data; Mothers; Polymerase Chain Reaction; Tooth

Adgang til dokumentet

10.1016/j.forsciint.2006.03.020

Citationsformater

@article{873338d09e4411df928f000ea68e967b,

title = "The last Viking King: a royal maternity case solved by ancient DNA analysis",

abstract = "The last of the Danish Viking Kings, Sven Estridsen, died in a.d. 1074 and is entombed in Roskilde Cathedral with other Danish kings and queens. Sven's mother, Estrid, is entombed in a pillar across the chancel. However, while there is no reasonable doubt about the identity of Sven, there have been doubts among historians whether the woman entombed was indeed Estrid. To shed light on this problem, we have extracted and analysed mitochondrial DNA (mtDNA) from pulp of teeth from each of the two royals. Four overlapping DNA-fragments covering about 400bp of hypervariable region 1 (HVR-1) of the D-loop were PCR amplified, cloned and a number of clones with each segment were sequenced. Also a segment containing the H/non-H specific nucleotide 7028 was sequenced. Consensus sequences were determined and D-loop results were replicated in an independent laboratory. This allowed the assignment of King Sven Estridsen to haplogroup H; Estrid's sequence differed from that of Sven at two positions in HVR-1, 16093T-->C and 16304T-->C, indicating that she belongs to subgroup H5a. Given the maternal inheritance of mtDNA, offspring will have the same mtDNA sequence as their mother with the exception of rare cases where the sequence has been altered by a germ line mutation. Therefore, the observation of two sequence differences makes it highly unlikely that the entombed woman was the mother of Sven. In addition, physical examination of the skeleton and the teeth strongly indicated that this woman was much younger (approximately 35 years) at the time of death than the 70 years history records tell. Although the entombed woman cannot be the Estrid, she may well be one of Sven's two daughters-in-law who were also called Estrid and who both became queens.",

author = "J{\o}rgen Dissing and Jonas Binladen and Anders Hansen and Birgitte Sejrsen and Eske Willerslev and Niels Lynnerup",

note = "Keywords: Base Sequence; DNA, Mitochondrial; Denmark; European Continental Ancestry Group; Female; Forensic Medicine; History, Ancient; Humans; Male; Molecular Sequence Data; Mothers; Polymerase Chain Reaction; Tooth",

year = "2006",

doi = "10.1016/j.forsciint.2006.03.020",

language = "English",

volume = "166",

pages = "21--7",

journal = "Forensic Science International",

issn = "0379-0738",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - The last Viking King: a royal maternity case solved by ancient DNA analysis

AU - Dissing, Jørgen

AU - Binladen, Jonas

AU - Hansen, Anders

AU - Sejrsen, Birgitte

AU - Willerslev, Eske

AU - Lynnerup, Niels

N1 - Keywords: Base Sequence; DNA, Mitochondrial; Denmark; European Continental Ancestry Group; Female; Forensic Medicine; History, Ancient; Humans; Male; Molecular Sequence Data; Mothers; Polymerase Chain Reaction; Tooth

PY - 2006

Y1 - 2006

N2 - The last of the Danish Viking Kings, Sven Estridsen, died in a.d. 1074 and is entombed in Roskilde Cathedral with other Danish kings and queens. Sven's mother, Estrid, is entombed in a pillar across the chancel. However, while there is no reasonable doubt about the identity of Sven, there have been doubts among historians whether the woman entombed was indeed Estrid. To shed light on this problem, we have extracted and analysed mitochondrial DNA (mtDNA) from pulp of teeth from each of the two royals. Four overlapping DNA-fragments covering about 400bp of hypervariable region 1 (HVR-1) of the D-loop were PCR amplified, cloned and a number of clones with each segment were sequenced. Also a segment containing the H/non-H specific nucleotide 7028 was sequenced. Consensus sequences were determined and D-loop results were replicated in an independent laboratory. This allowed the assignment of King Sven Estridsen to haplogroup H; Estrid's sequence differed from that of Sven at two positions in HVR-1, 16093T-->C and 16304T-->C, indicating that she belongs to subgroup H5a. Given the maternal inheritance of mtDNA, offspring will have the same mtDNA sequence as their mother with the exception of rare cases where the sequence has been altered by a germ line mutation. Therefore, the observation of two sequence differences makes it highly unlikely that the entombed woman was the mother of Sven. In addition, physical examination of the skeleton and the teeth strongly indicated that this woman was much younger (approximately 35 years) at the time of death than the 70 years history records tell. Although the entombed woman cannot be the Estrid, she may well be one of Sven's two daughters-in-law who were also called Estrid and who both became queens.

AB - The last of the Danish Viking Kings, Sven Estridsen, died in a.d. 1074 and is entombed in Roskilde Cathedral with other Danish kings and queens. Sven's mother, Estrid, is entombed in a pillar across the chancel. However, while there is no reasonable doubt about the identity of Sven, there have been doubts among historians whether the woman entombed was indeed Estrid. To shed light on this problem, we have extracted and analysed mitochondrial DNA (mtDNA) from pulp of teeth from each of the two royals. Four overlapping DNA-fragments covering about 400bp of hypervariable region 1 (HVR-1) of the D-loop were PCR amplified, cloned and a number of clones with each segment were sequenced. Also a segment containing the H/non-H specific nucleotide 7028 was sequenced. Consensus sequences were determined and D-loop results were replicated in an independent laboratory. This allowed the assignment of King Sven Estridsen to haplogroup H; Estrid's sequence differed from that of Sven at two positions in HVR-1, 16093T-->C and 16304T-->C, indicating that she belongs to subgroup H5a. Given the maternal inheritance of mtDNA, offspring will have the same mtDNA sequence as their mother with the exception of rare cases where the sequence has been altered by a germ line mutation. Therefore, the observation of two sequence differences makes it highly unlikely that the entombed woman was the mother of Sven. In addition, physical examination of the skeleton and the teeth strongly indicated that this woman was much younger (approximately 35 years) at the time of death than the 70 years history records tell. Although the entombed woman cannot be the Estrid, she may well be one of Sven's two daughters-in-law who were also called Estrid and who both became queens.

U2 - 10.1016/j.forsciint.2006.03.020

DO - 10.1016/j.forsciint.2006.03.020

M3 - Journal article

C2 - 16687224

VL - 166

SP - 21

EP - 27

JO - Forensic Science International

JF - Forensic Science International

SN - 0379-0738

IS - 1

ER -