Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Histochemistry and Cell Biology |
| Vol/bind | 130 |
| Udgave nummer | 2 |
| Sider (fra-til) | 363-73 |
| Antal sider | 10 |
| ISSN | 0948-6143 |
| DOI | |
| Status | Udgivet - 2008 |
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I: Histochemistry and Cell Biology, Bind 130, Nr. 2, 2008, s. 363-73.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - The macrophage system in the intestinal muscularis externa during inflammation: an immunohistochemical and quantitative study of osteopetrotic mice.
AU - Mikkelsen, Hanne Birte
AU - Larsen, Jytte Overgaard
AU - Hadberg, Hanne
PY - 2008
Y1 - 2008
N2 - Intestinal inflammation results in disturbed intestinal motility in humans as well as in animal models. This altered function of smooth muscle cells and/or the enteric nervous system may be caused by activation of macrophages in muscularis externa and a thereby following release of cytokines and chemokines that causes influx of mononuclear cells and neutrophilic granulocytes. We subjected osteopetrotic (op/op) mice that lack certain macrophage subtypes, e.g. macrophages in the muscularis externa and +/+ mice to LPS to induce inflammatory cell influx. The densities of F4/80(+), MHCII(+), and myeloperoxidase(+) cells were quantified using stereological sampling. In +/+ mice we found that MHCII(+) cells outnumber F4/80(+) cells and that LPS injection increased the density of MHCII(+) cells temporarily but not that of F4/80(+) cells. This indicates that an upregulation of MHCII antigen takes place and that two or more macrophage subtypes with comparable morphologies exist. Osteopetrotic mice lacked MHCII(+), CD169(+), and F4/80(+) cells after either treatment, which indicate that these cells are CSF-1-dependent. LPS induced VCAM-1 activation of the vessels, modest influx of granulocytes, as well as an iNOS-activation in a cell type different from macrophages in both +/+ and op/op mice.
AB - Intestinal inflammation results in disturbed intestinal motility in humans as well as in animal models. This altered function of smooth muscle cells and/or the enteric nervous system may be caused by activation of macrophages in muscularis externa and a thereby following release of cytokines and chemokines that causes influx of mononuclear cells and neutrophilic granulocytes. We subjected osteopetrotic (op/op) mice that lack certain macrophage subtypes, e.g. macrophages in the muscularis externa and +/+ mice to LPS to induce inflammatory cell influx. The densities of F4/80(+), MHCII(+), and myeloperoxidase(+) cells were quantified using stereological sampling. In +/+ mice we found that MHCII(+) cells outnumber F4/80(+) cells and that LPS injection increased the density of MHCII(+) cells temporarily but not that of F4/80(+) cells. This indicates that an upregulation of MHCII antigen takes place and that two or more macrophage subtypes with comparable morphologies exist. Osteopetrotic mice lacked MHCII(+), CD169(+), and F4/80(+) cells after either treatment, which indicate that these cells are CSF-1-dependent. LPS induced VCAM-1 activation of the vessels, modest influx of granulocytes, as well as an iNOS-activation in a cell type different from macrophages in both +/+ and op/op mice.
U2 - 10.1007/s00418-008-0423-x
DO - 10.1007/s00418-008-0423-x
M3 - Journal article
C2 - 18392842
SN - 0948-6143
VL - 130
SP - 363
EP - 373
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
IS - 2
ER -