@article{93a9a7b053dd11dd8d9f000ea68e967b,
title = "The promyelocytic leukemia gene product (PML) forms stable complexes with the retinoblastoma protein.",
abstract = "PML is a nuclear protein with growth-suppressive properties originally identified in the context of the PML-retinoic acid receptor alpha (RAR alpha) fusion protein of acute promyelocytic leukemia. PML localizes within distinct nuclear structures, called nuclear bodies, which are disrupted by the expression of PML-RAR alpha. We report that PML colocalizes with the nonphosphorylated fraction of the retinoblastoma protein (pRB) within nuclear bodies and that pRB is delocalized by PML-RAR alpha expression. Both PML and PML-RAR alpha form complexes with the nonphosphorylated form of pRB in vivo, and they interact with the pocket region of pRB. The regions of PML and PML-RAR alpha involved in pRB binding differ; in fact, the B boxes and the C-terminal region of PML, the latter of which is not present in PML-RAR alpha, are essential for the formation of stable complexes with pRB. Functionally, PML abolishes activation of glucocorticoid receptor-regulated transcription by pRB, whereas PML-RAR alpha further increases it. Our results suggest that PML may be part of transcription-regulatory complexes and that the oncogenic potential of the PML-RAR alpha protein may derive from the alteration of PML-regulated transcription.",
author = "M Alcalay and L Tomassoni and E Colombo and S Stoldt and F Grignani and M Fagioli and L Szekely and K Helin and Pelicci, {P G}",
note = "Keywords: Cell Division; Humans; Inclusion Bodies; Macromolecular Substances; Neoplasm Proteins; Nuclear Proteins; Oncogene Proteins, Fusion; Promoter Regions (Genetics); Protein Binding; Receptors, Glucocorticoid; Retinoblastoma Protein; Transcription Factors; Transcription, Genetic; Tumor Cells, Cultured; Tumor Suppressor Proteins",
year = "1998",
language = "English",
volume = "18",
pages = "1084--93",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "2",
}