Three genes preceding pyrE on the Escherichia coli chromosome are essential for survival and normal cell morphology in stationary culture and at high temperature

Peter Poulsen; Kaj Frank Jensen

doi:10.1016/0923-2508(91)90042-9

Three genes preceding pyrE on the Escherichia coli chromosome are essential for survival and normal cell morphology in stationary culture and at high temperature

Peter Poulsen, Kaj Frank Jensen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

16 Citationer (Scopus)

Abstract

Udgivelsesdato: 1991

Originalsprog	Engelsk
Tidsskrift	Research in Microbiology
Vol/bind	142
Udgave nummer	2-3
Sider (fra-til)	283-288
ISSN	0923-2508
DOI	https://doi.org/10.1016/0923-2508(91)90042-9
Status	Udgivet - 1991

Bibliografisk note

Author Keywords: orf gene, pyrE, E. coli; Keratin-like protein, Heat-sensitive growth, Stationary culture

Adgang til dokumentet

10.1016/0923-2508(91)90042-9

Citationsformater

@article{109b1f3074d111dbbee902004c4f4f50,

title = "Three genes preceding pyrE on the Escherichia coli chromosome are essential for survival and normal cell morphology in stationary culture and at high temperature",

abstract = "Previous studies of the upstream region of the pyrE gene in Escherichia coli revealed three genes of unknown function. Inactivation of these genes (designated orfE, orfX and orfY) by crossing the KmR-cassette-disrupted orf into the chromosome indicated that they were not required during exponential growth (Poulsen et al., Mol., Microbiol., 1989 b). Here we report that the three genes are of importance in the stationary phase. Thus, cultures of the mutants grown to a stationary state in rich media contained bacterial filaments of abnormal morphology. In addition, flow cytometric analyses showed that outgrown cultures of the orf mutants have anomalous size distribution and DNA content, and that rifampicin treatment of exponentially growing mutants results in cell populations with chromosome numbers in the range from about 1 to 10, compared with wild type strains that end up with 4 and 8 full chromosomes.Finally, it appeared that the three orf's are indispensable at high temperatures, since the insertion mutants were unable to form colonies above 45°C and since cultures of exponentially growing mutants lysed upon a temperature shift from 37°C to 45°C.",

author = "Peter Poulsen and Jensen, {Kaj Frank}",

note = "Author Keywords: orf gene, pyrE, E. coli; Keratin-like protein, Heat-sensitive growth, Stationary culture",

year = "1991",

doi = "10.1016/0923-2508(91)90042-9",

language = "English",

volume = "142",

pages = "283--288",

journal = "Research in Microbiology",

issn = "0923-2508",

publisher = "Elsevier Masson",

number = "2-3",

}

TY - JOUR

T1 - Three genes preceding pyrE on the Escherichia coli chromosome are essential for survival and normal cell morphology in stationary culture and at high temperature

AU - Poulsen, Peter

AU - Jensen, Kaj Frank

N1 - Author Keywords: orf gene, pyrE, E. coli; Keratin-like protein, Heat-sensitive growth, Stationary culture

PY - 1991

Y1 - 1991

N2 - Previous studies of the upstream region of the pyrE gene in Escherichia coli revealed three genes of unknown function. Inactivation of these genes (designated orfE, orfX and orfY) by crossing the KmR-cassette-disrupted orf into the chromosome indicated that they were not required during exponential growth (Poulsen et al., Mol., Microbiol., 1989 b). Here we report that the three genes are of importance in the stationary phase. Thus, cultures of the mutants grown to a stationary state in rich media contained bacterial filaments of abnormal morphology. In addition, flow cytometric analyses showed that outgrown cultures of the orf mutants have anomalous size distribution and DNA content, and that rifampicin treatment of exponentially growing mutants results in cell populations with chromosome numbers in the range from about 1 to 10, compared with wild type strains that end up with 4 and 8 full chromosomes.Finally, it appeared that the three orf's are indispensable at high temperatures, since the insertion mutants were unable to form colonies above 45°C and since cultures of exponentially growing mutants lysed upon a temperature shift from 37°C to 45°C.

AB - Previous studies of the upstream region of the pyrE gene in Escherichia coli revealed three genes of unknown function. Inactivation of these genes (designated orfE, orfX and orfY) by crossing the KmR-cassette-disrupted orf into the chromosome indicated that they were not required during exponential growth (Poulsen et al., Mol., Microbiol., 1989 b). Here we report that the three genes are of importance in the stationary phase. Thus, cultures of the mutants grown to a stationary state in rich media contained bacterial filaments of abnormal morphology. In addition, flow cytometric analyses showed that outgrown cultures of the orf mutants have anomalous size distribution and DNA content, and that rifampicin treatment of exponentially growing mutants results in cell populations with chromosome numbers in the range from about 1 to 10, compared with wild type strains that end up with 4 and 8 full chromosomes.Finally, it appeared that the three orf's are indispensable at high temperatures, since the insertion mutants were unable to form colonies above 45°C and since cultures of exponentially growing mutants lysed upon a temperature shift from 37°C to 45°C.

U2 - 10.1016/0923-2508(91)90042-9

DO - 10.1016/0923-2508(91)90042-9

M3 - Journal article

SN - 0923-2508

VL - 142

SP - 283

EP - 288

JO - Research in Microbiology

JF - Research in Microbiology

IS - 2-3

ER -