Time of action of 4.5 S RNA in Escherichia coli translation

S Brown

Time of action of 4.5 S RNA in Escherichia coli translation

S Brown

Bioinformatik og RNA Biologi

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

53 Citationer (Scopus)

Abstract

Udgivelsesdato: 1989-Sep-5

Originalsprog	Engelsk
Tidsskrift	Journal of Molecular Biology
Vol/bind	209
Udgave nummer	1
Sider (fra-til)	79-90
Antal sider	11
ISSN	0022-2836
Status	Udgivet - 1989

Bibliografisk note

Keywords: Anti-Bacterial Agents; Escherichia coli; Genes, Bacterial; Glutamate-tRNA Ligase; Mutation; Protein Biosynthesis; RNA, Bacterial; RNA, Ribosomal; RNA, Transfer; RNA, Transfer, Amino Acyl; Suppression, Genetic; Valine-tRNA Ligase

Citationsformater

@article{f5390020d29011dd9473000ea68e967b,

title = "Time of action of 4.5 S RNA in Escherichia coli translation",

abstract = "A new class of suppressor mutants helps to define the role of 4.5 S RNA in translation. The suppressors reduce the requirement for 4.5 S RNA by increasing the intracellular concentration of uncharged tRNA. Suppression probably occurs by prolonging the period in which translating ribosomes have translocated but not yet released the uncharged tRNA, indicating that this is the point at which 4.5 S RNA enters translation. The release of 4.5 S RNA from polysomes is affected by antibiotics that inhibit protein synthesis. The antibiotic-sensitivity of this release indicates that 4.5 S RNA exits the ribosome following translocation and prior to release of protein synthesis elongation factor G. These results indicate that 4.5 S RNA acts immediately after ribosomal translocation. A model is proposed in which 4.5 S RNA stabilizes the post-translocation state by replacing 23 S ribosomal RNA as a binding site for elongation factor G. The 4.5 S RNA-requirement of mutants altered in 23 S ribosomal RNA support this model.",

author = "S Brown",

note = "Keywords: Anti-Bacterial Agents; Escherichia coli; Genes, Bacterial; Glutamate-tRNA Ligase; Mutation; Protein Biosynthesis; RNA, Bacterial; RNA, Ribosomal; RNA, Transfer; RNA, Transfer, Amino Acyl; Suppression, Genetic; Valine-tRNA Ligase",

year = "1989",

language = "English",

volume = "209",

pages = "79--90",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press",

number = "1",

}

TY - JOUR

T1 - Time of action of 4.5 S RNA in Escherichia coli translation

AU - Brown, S

N1 - Keywords: Anti-Bacterial Agents; Escherichia coli; Genes, Bacterial; Glutamate-tRNA Ligase; Mutation; Protein Biosynthesis; RNA, Bacterial; RNA, Ribosomal; RNA, Transfer; RNA, Transfer, Amino Acyl; Suppression, Genetic; Valine-tRNA Ligase

PY - 1989

Y1 - 1989

N2 - A new class of suppressor mutants helps to define the role of 4.5 S RNA in translation. The suppressors reduce the requirement for 4.5 S RNA by increasing the intracellular concentration of uncharged tRNA. Suppression probably occurs by prolonging the period in which translating ribosomes have translocated but not yet released the uncharged tRNA, indicating that this is the point at which 4.5 S RNA enters translation. The release of 4.5 S RNA from polysomes is affected by antibiotics that inhibit protein synthesis. The antibiotic-sensitivity of this release indicates that 4.5 S RNA exits the ribosome following translocation and prior to release of protein synthesis elongation factor G. These results indicate that 4.5 S RNA acts immediately after ribosomal translocation. A model is proposed in which 4.5 S RNA stabilizes the post-translocation state by replacing 23 S ribosomal RNA as a binding site for elongation factor G. The 4.5 S RNA-requirement of mutants altered in 23 S ribosomal RNA support this model.

AB - A new class of suppressor mutants helps to define the role of 4.5 S RNA in translation. The suppressors reduce the requirement for 4.5 S RNA by increasing the intracellular concentration of uncharged tRNA. Suppression probably occurs by prolonging the period in which translating ribosomes have translocated but not yet released the uncharged tRNA, indicating that this is the point at which 4.5 S RNA enters translation. The release of 4.5 S RNA from polysomes is affected by antibiotics that inhibit protein synthesis. The antibiotic-sensitivity of this release indicates that 4.5 S RNA exits the ribosome following translocation and prior to release of protein synthesis elongation factor G. These results indicate that 4.5 S RNA acts immediately after ribosomal translocation. A model is proposed in which 4.5 S RNA stabilizes the post-translocation state by replacing 23 S ribosomal RNA as a binding site for elongation factor G. The 4.5 S RNA-requirement of mutants altered in 23 S ribosomal RNA support this model.

M3 - Journal article

C2 - 2478715

VL - 209

SP - 79

EP - 90

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 1

ER -