To Be or Not to Be: Circular RNAs or mRNAs From Circular DNAs?

Leire Iparraguirre, Iñigo Prada-Luengo, Birgitte Regenberg, David Otaegui

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

14 Citationer (Scopus)
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Abstract

In recent years, there has been a growing interest in circular RNAs (circRNAs) since they are involved in a wide spectrum of cellular functions that might have a large impact on phenotype and disease. CircRNAs are mainly recorded by RNA-Seq and computational methods focused on the detection of back-splicing junction sequences considered the diagnostic feature of circRNAs. While some protocols remove linear RNA prior to sequencing, many have characterized circRNAs by sorting through total RNA sequencing data without excluding the possibility that some linear RNA can provide the same signal as a circRNA. Recent studies have revealed that circular DNAs of chromosomal origin are common in eukaryotic genomes and that they can be transcribed. Transcription events across the junction of circular DNAs would result in a transcript with a junction similar to those present in circRNAs. Therefore, in this report, we want to draw attention to transcripts from such circular DNAs both as an interesting new player in the transcriptome and also as a confounding factor that must be taken into account when studying circRNAs.

OriginalsprogEngelsk
Artikelnummer940
TidsskriftFrontiers in Genetics
Vol/bind10
Antal sider6
ISSN1664-8021
DOI
StatusUdgivet - 2019

Bibliografisk note

Copyright © 2019 Iparraguirre, Prada-Luengo, Regenberg and Otaegui.

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