TY - JOUR
T1 - Transfer of gut microbiota from lean and obese mice to antibiotic-treated mice
AU - Ellekilde, Merete
AU - Selfjord, Ellika Marie
AU - Larsen, Christian Schiøth
AU - Jakesevic, Maja
AU - Rune, Ida
AU - Christensen, Britt Tranberg
AU - Vogensen, Finn Kvist
AU - Nielsen, Dennis Sandris
AU - Bahl, Martin Iain
AU - Licht, Tine Rask
AU - Hansen, Axel Kornerup
AU - Hansen, Camilla Hartmann Friis
PY - 2014
Y1 - 2014
N2 - Transferring gut microbiota from one individual to another may enable researchers to "humanize" the gut of animal models and transfer phenotypes between species. To date, most studies of gut microbiota transfer are performed in germ-free mice. In the studies presented, it was tested whether an antibiotic treatment approach could be used instead. C57BL/6 mice were treated with ampicillin prior to inoculation at weaning or eight weeks of age with gut microbiota from lean or obese donors. The gut microbiota and clinical parameters of the recipients was characterized one and six weeks after inoculation. The results demonstrate, that the donor gut microbiota was introduced, established, and changed the gut microbiota of the recipients. Six weeks after inoculation, the differences persisted, however alteration of the gut microbiota occurred with time within the groups. The clinical parameters of the donor phenotype were partly transmissible from obese to lean mice, in particularly β cell hyperactivity in the obese recipients. Thus, a successful inoculation of gut microbiota was not age dependent in order for the microbes to colonize, and transferring different microbial compositions to conventional antibiotic-treated mice was possible at least for a time period during which the microbiota may permanently modulate important host functions.
AB - Transferring gut microbiota from one individual to another may enable researchers to "humanize" the gut of animal models and transfer phenotypes between species. To date, most studies of gut microbiota transfer are performed in germ-free mice. In the studies presented, it was tested whether an antibiotic treatment approach could be used instead. C57BL/6 mice were treated with ampicillin prior to inoculation at weaning or eight weeks of age with gut microbiota from lean or obese donors. The gut microbiota and clinical parameters of the recipients was characterized one and six weeks after inoculation. The results demonstrate, that the donor gut microbiota was introduced, established, and changed the gut microbiota of the recipients. Six weeks after inoculation, the differences persisted, however alteration of the gut microbiota occurred with time within the groups. The clinical parameters of the donor phenotype were partly transmissible from obese to lean mice, in particularly β cell hyperactivity in the obese recipients. Thus, a successful inoculation of gut microbiota was not age dependent in order for the microbes to colonize, and transferring different microbial compositions to conventional antibiotic-treated mice was possible at least for a time period during which the microbiota may permanently modulate important host functions.
U2 - 10.1038/srep05922
DO - 10.1038/srep05922
M3 - Journal article
C2 - 25082483
VL - 4
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 5922
ER -