Transparent polymeric cell culture chip with integrated temperature control and uniform media perfusion

Sarunas Petronis; Michael Stangegaard; Claus Bo Vöge Christensend; Hans Martin Dufva

doi:0736-6205

Transparent polymeric cell culture chip with integrated temperature control and uniform media perfusion

Sarunas Petronis, Michael Stangegaard, Claus Bo Vöge Christensend, Hans Martin Dufva

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

76 Citationer (Scopus)

Abstract

Udgivelsesdato: March 2006

Originalsprog	Engelsk
Tidsskrift	BioTechniques
Vol/bind	40
Udgave nummer	3
Sider (fra-til)	368-376
Antal sider	8
ISSN	0736-6205
DOI	https://doi.org/0736-6205
Status	Udgivet - 2006
Udgivet eksternt	Ja

Adgang til dokumentet

0736-6205

Citationsformater

@article{3e765fd0af3b11debc73000ea68e967b,

title = "Transparent polymeric cell culture chip with integrated temperature control and uniform media perfusion",

abstract = "Modern microfabrication and microfluidic technologies offer new opportunities in the design and fabrication of miniaturized cell culture systems for online monitoring of living cells. We used laser micromachining and thermal bonding to fabricate an optically transparent, low-cost polymeric chip for long-term online cell culture observation under controlled conditions. The chip incorporated a microfluidic flow equalization system, assuring uniform perfusion of the cell culture media throughout the cell culture chamber. The integrated indium-tin-oxide heater and miniature temperature probe linked to an electronic feedback system created steady and spatially uniform thermal conditions with minimal interference to the optical transparency of the chip. The fluidic and thermal performance of the chip was verified by finite element modeling and by operation tests under fluctuating ambient temperature conditions. HeLa cells were cultured for up to 2 weeks within the cell culture chip and monitored using a time-lapse video recording microscopy setup. Cell attachment and spreading was observed during the first 10-20 h (lag phase). After approximately 20 h, cell growth gained exponential character with an estimated doubling time of about 32 h, which is identical to the observed doubling time of cells grown in standard cell culture flasks in a CO2 incubator.",

author = "Sarunas Petronis and Michael Stangegaard and Christensend, {Claus Bo V{\"o}ge} and Dufva, {Hans Martin}",

year = "2006",

doi = "0736-6205",

language = "English",

volume = "40",

pages = "368--376",

journal = "BioTechniques",

issn = "0736-6205",

publisher = "Informa Healthcare",

number = "3",

}

TY - JOUR

T1 - Transparent polymeric cell culture chip with integrated temperature control and uniform media perfusion

AU - Petronis, Sarunas

AU - Stangegaard, Michael

AU - Christensend, Claus Bo Vöge

AU - Dufva, Hans Martin

PY - 2006

Y1 - 2006

N2 - Modern microfabrication and microfluidic technologies offer new opportunities in the design and fabrication of miniaturized cell culture systems for online monitoring of living cells. We used laser micromachining and thermal bonding to fabricate an optically transparent, low-cost polymeric chip for long-term online cell culture observation under controlled conditions. The chip incorporated a microfluidic flow equalization system, assuring uniform perfusion of the cell culture media throughout the cell culture chamber. The integrated indium-tin-oxide heater and miniature temperature probe linked to an electronic feedback system created steady and spatially uniform thermal conditions with minimal interference to the optical transparency of the chip. The fluidic and thermal performance of the chip was verified by finite element modeling and by operation tests under fluctuating ambient temperature conditions. HeLa cells were cultured for up to 2 weeks within the cell culture chip and monitored using a time-lapse video recording microscopy setup. Cell attachment and spreading was observed during the first 10-20 h (lag phase). After approximately 20 h, cell growth gained exponential character with an estimated doubling time of about 32 h, which is identical to the observed doubling time of cells grown in standard cell culture flasks in a CO2 incubator.

AB - Modern microfabrication and microfluidic technologies offer new opportunities in the design and fabrication of miniaturized cell culture systems for online monitoring of living cells. We used laser micromachining and thermal bonding to fabricate an optically transparent, low-cost polymeric chip for long-term online cell culture observation under controlled conditions. The chip incorporated a microfluidic flow equalization system, assuring uniform perfusion of the cell culture media throughout the cell culture chamber. The integrated indium-tin-oxide heater and miniature temperature probe linked to an electronic feedback system created steady and spatially uniform thermal conditions with minimal interference to the optical transparency of the chip. The fluidic and thermal performance of the chip was verified by finite element modeling and by operation tests under fluctuating ambient temperature conditions. HeLa cells were cultured for up to 2 weeks within the cell culture chip and monitored using a time-lapse video recording microscopy setup. Cell attachment and spreading was observed during the first 10-20 h (lag phase). After approximately 20 h, cell growth gained exponential character with an estimated doubling time of about 32 h, which is identical to the observed doubling time of cells grown in standard cell culture flasks in a CO2 incubator.

U2 - 0736-6205

DO - 0736-6205

M3 - Journal article

SN - 0736-6205

VL - 40

SP - 368

EP - 376

JO - BioTechniques

JF - BioTechniques

IS - 3

ER -