Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Endocrinology |
| Vol/bind | 147 |
| Udgave nummer | 12 |
| Sider (fra-til) | 5752-9 |
| Antal sider | 7 |
| ISSN | 0013-7227 |
| DOI | |
| Status | Udgivet - 2006 |
Bibliografisk note
Keywords: Adult; Animals; Animals, Newborn; Apoptosis; Cell Movement; Cell Proliferation; Gene Expression Regulation; Growth Hormone; Humans; Insulin; Insulinoma; Islets of Langerhans; Pancreatic Neoplasms; Peptides; Rats; Tissue Distribution; Tumor Cells, CulturedAdgang til dokumentet
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Trefoil factors are expressed in human and rat endocrine pancreas: differential regulation by growth hormone. / Jackerott, Malene; Lee, Ying C; Møllgård, Kjeld; Kofod, Hans; Jensen, Janne; Rohleder, Sonja; Neubauer, Nicole; Gaarn, Louise W; Lykke, Jeanette; Dodge, Rikke; Dalgaard, Louise T; Søstrup, Birgitte; Jensen, Dennis B; Thim, Lars; Nexø, Ebba; Thams, Peter; Bisgaard, Hanne Cathrine; Nielsen, Jens H.
I: Endocrinology, Bind 147, Nr. 12, 2006, s. 5752-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Trefoil factors are expressed in human and rat endocrine pancreas: differential regulation by growth hormone.
AU - Jackerott, Malene
AU - Lee, Ying C
AU - Møllgård, Kjeld
AU - Kofod, Hans
AU - Jensen, Janne
AU - Rohleder, Sonja
AU - Neubauer, Nicole
AU - Gaarn, Louise W
AU - Lykke, Jeanette
AU - Dodge, Rikke
AU - Dalgaard, Louise T
AU - Søstrup, Birgitte
AU - Jensen, Dennis B
AU - Thim, Lars
AU - Nexø, Ebba
AU - Thams, Peter
AU - Bisgaard, Hanne Cathrine
AU - Nielsen, Jens H
N1 - Keywords: Adult; Animals; Animals, Newborn; Apoptosis; Cell Movement; Cell Proliferation; Gene Expression Regulation; Growth Hormone; Humans; Insulin; Insulinoma; Islets of Langerhans; Pancreatic Neoplasms; Peptides; Rats; Tissue Distribution; Tumor Cells, Cultured
PY - 2006
Y1 - 2006
N2 - Trefoil factors (TFFs) 1, 2, and 3 are expressed in mucosal epithelia. TFFs are particular abundant in the intestine in which they play a crucial role in maintenance and restitution of the epithelium. Because pancreas developmentally arises from the primitive foregut, we explored the expression of TFFs in the pancreas in man and rat. Immunocytochemical staining of adult human pancreas showed abundant TFF3 immunoreactivity in pancreatic islets and some duct cells, whereas weak TFF1 and no TFF2 staining were detected. In the islets TFF3 localized to most insulin and some glucagon and pancreatic polypeptide-producing cells. TFF3 immunoreactivity was colocalized with insulin and glucagon in distinct cell clusters in human fetal pancreas at wk 14 and in the newborn rat pancreas. In isolated human and rat islets, TFF3 and TFF1 mRNA was identified by RT-PCR, and TFF3 protein was detected in human pancreas and islets by ELISA. Exposure of neonatal rat islets or insulinoma cells to GH, a known beta-cell growth factor, resulted in markedly increased TFF3 but decreased TFF1 mRNA levels. The effect of GH on TFF3 expression was confirmed by Western blot. Culture of neonatal rat islets in the presence of TFF3 resulted in attachment and migration of the islet cells, but no effects on proliferation, insulin secretion or cytokine-induced apoptosis were seen. These data demonstrate expression of TFFs in the endocrine pancreas, but their possible functions remain unknown.
AB - Trefoil factors (TFFs) 1, 2, and 3 are expressed in mucosal epithelia. TFFs are particular abundant in the intestine in which they play a crucial role in maintenance and restitution of the epithelium. Because pancreas developmentally arises from the primitive foregut, we explored the expression of TFFs in the pancreas in man and rat. Immunocytochemical staining of adult human pancreas showed abundant TFF3 immunoreactivity in pancreatic islets and some duct cells, whereas weak TFF1 and no TFF2 staining were detected. In the islets TFF3 localized to most insulin and some glucagon and pancreatic polypeptide-producing cells. TFF3 immunoreactivity was colocalized with insulin and glucagon in distinct cell clusters in human fetal pancreas at wk 14 and in the newborn rat pancreas. In isolated human and rat islets, TFF3 and TFF1 mRNA was identified by RT-PCR, and TFF3 protein was detected in human pancreas and islets by ELISA. Exposure of neonatal rat islets or insulinoma cells to GH, a known beta-cell growth factor, resulted in markedly increased TFF3 but decreased TFF1 mRNA levels. The effect of GH on TFF3 expression was confirmed by Western blot. Culture of neonatal rat islets in the presence of TFF3 resulted in attachment and migration of the islet cells, but no effects on proliferation, insulin secretion or cytokine-induced apoptosis were seen. These data demonstrate expression of TFFs in the endocrine pancreas, but their possible functions remain unknown.
U2 - 10.1210/en.2006-0601
DO - 10.1210/en.2006-0601
M3 - Journal article
C2 - 16973727
VL - 147
SP - 5752
EP - 5759
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0013-7227
IS - 12
ER -