Type IV CRISPR-Cas systems are highly diverse and involved in competition between plasmids

Rafael Pinilla-Redondo, David Mayo-Muñoz, Jakob Russel, Roger A. Garrett, Lennart Randau, Søren J. Sørensen, Shiraz A. Shah

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

24 Citationer (Scopus)
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Abstract

CRISPR-Cas systems provide prokaryotes with adaptive immune functions against viruses and other genetic parasites. In contrast to all other types of CRISPR-Cas systems, type IV has remained largely overlooked. Here, we describe a previously uncharted diversity of type IV gene cassettes, primarily encoded by plasmid-like elements from diverse prokaryotic taxa. Remarkably, via a comprehensive analysis of their CRISPR spacer content, these systems were found to exhibit a strong bias towards the targeting of other plasmids. Our data indicate that the functions of type IV systems have diverged from those of other host-related CRISPR-Cas immune systems to adopt a role in mediating conflicts between plasmids. Furthermore, we find evidence for cross-talk between certain type IV and type I CRISPR-Cas systems that co-exist intracellularly, thus providing a simple answer to the enigmatic absence of type IV adaptation modules. Collectively, our results lead to the expansion and reclassification of type IV systems and provide novel insights into the biological function and evolution of these elusive systems.

OriginalsprogEngelsk
TidsskriftNucleic Acids Research
Vol/bind48
Udgave nummer4
Sider (fra-til)2000-2012
Antal sider13
ISSN0305-1048
DOI
StatusUdgivet - 2020

Bibliografisk note

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

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