Unexpected Diagnosis of Cerebral Toxoplasmosis by 16S and D2 Large-Subunit Ribosomal DNA PCR and Sequencing

Alexandra Y C Kruse; Lasse Kvich; Steffen Eickhardt; Lars H Omland; Thomas Bjarnsholt; Claus Moser

doi:10.1128/JCM.03404-14

Unexpected Diagnosis of Cerebral Toxoplasmosis by 16S and D2 Large-Subunit Ribosomal DNA PCR and Sequencing

Alexandra Y C Kruse, Lasse Kvich, Steffen Eickhardt, Lars H Omland, Thomas Bjarnsholt, Claus Moser

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstract

The protozoan parasite Toxoplasma gondii causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis. T. gondii was diagnosed by 16S and D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing of a cerebral biopsy specimen and confirmed by T. gondii-specific PCR and immunohistochemistry. The patient was later diagnosed with HIV/AIDS.

Originalsprog	Engelsk
Tidsskrift	Journal of clinical microbiology
Vol/bind	53
Udgave nummer	6
Sider (fra-til)	1983-5
Antal sider	3
ISSN	0095-1137
DOI	https://doi.org/10.1128/JCM.03404-14
Status	Udgivet - jun. 2015

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Citationsformater

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title = "Unexpected Diagnosis of Cerebral Toxoplasmosis by 16S and D2 Large-Subunit Ribosomal DNA PCR and Sequencing",

abstract = "The protozoan parasite Toxoplasma gondii causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis. T. gondii was diagnosed by 16S and D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing of a cerebral biopsy specimen and confirmed by T. gondii-specific PCR and immunohistochemistry. The patient was later diagnosed with HIV/AIDS.",

keywords = "AIDS-Related Opportunistic Infections/diagnosis, Aged, Base Sequence, Brain/pathology, DNA, Protozoan/analysis, DNA, Ribosomal/analysis, Humans, Male, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Alignment, Toxoplasmosis, Cerebral/diagnosis",

author = "Kruse, {Alexandra Y C} and Lasse Kvich and Steffen Eickhardt and Omland, {Lars H} and Thomas Bjarnsholt and Claus Moser",

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language = "English",

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publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Unexpected Diagnosis of Cerebral Toxoplasmosis by 16S and D2 Large-Subunit Ribosomal DNA PCR and Sequencing

AU - Kruse, Alexandra Y C

AU - Kvich, Lasse

AU - Eickhardt, Steffen

AU - Omland, Lars H

AU - Bjarnsholt, Thomas

AU - Moser, Claus

PY - 2015/6

Y1 - 2015/6

N2 - The protozoan parasite Toxoplasma gondii causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis. T. gondii was diagnosed by 16S and D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing of a cerebral biopsy specimen and confirmed by T. gondii-specific PCR and immunohistochemistry. The patient was later diagnosed with HIV/AIDS.

AB - The protozoan parasite Toxoplasma gondii causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis. T. gondii was diagnosed by 16S and D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing of a cerebral biopsy specimen and confirmed by T. gondii-specific PCR and immunohistochemistry. The patient was later diagnosed with HIV/AIDS.

KW - AIDS-Related Opportunistic Infections/diagnosis

KW - Aged

KW - Base Sequence

KW - Brain/pathology

KW - DNA, Protozoan/analysis

KW - DNA, Ribosomal/analysis

KW - Humans

KW - Male

KW - Molecular Sequence Data

KW - Polymerase Chain Reaction

KW - Sequence Alignment

KW - Toxoplasmosis, Cerebral/diagnosis

U2 - 10.1128/JCM.03404-14

DO - 10.1128/JCM.03404-14

M3 - Journal article

C2 - 25854484

SN - 0095-1137

VL - 53

SP - 1983

EP - 1985

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 6

ER -