@article{66feddf054ac11dd8d9f000ea68e967b,
title = "v-erbA overexpression is required to extinguish c-erbA function in erythroid cell differentiation and regulation of the erbA target gene CAII.",
abstract = "The v-erbA oncoprotein represents a retrovirus-transduced oncogenic version of the thyroid hormone (T3/T4) receptor c-erbA (type alpha). It contributes to virus-induced erythroleukemia by efficiently arresting differentiation of red cell progenitors and by suppressing transcription of erythrocyte-specific genes. Here, we show that v-erbA and c-erbA bind directly to sequences within the promoter of the erythrocyte-specific carbonic anhydrase II (CAII), a gene whose transcription is efficiently suppressed by v-erbA. This erbA-binding site confers thyroid hormone responsiveness to a heterologous promoter in transient expression experiments and is a target for efficient down-regulation of CAII transcription by the v-erbA oncoprotein. In stably transformed erythroblasts coexpressing the v-erbA oncoprotein and the c-erbA/T3 receptor at an approximately equimolar ratio, c-erbA activity is dominant over v-erbA. T3 efficiently induced erythroid differentiation in these cells, thus overcoming the v-erbA-mediated differentiation arrest. Likewise, T3 activated CAII transcription as well as transient expression of a T3-responsive reporter gene containing the CAII-specific erbA-binding site. The c-erbA-dependent activation of this CAII reporter construct could only be suppressed by very high amounts of v-erbA. Our results suggest that overexpression of v-erbA is required for its function as an oncoprotein.",
author = "C Disela and C Glineur and T Bugge and J Sap and G Stengl and J Dodgson and H Stunnenberg and H Beug and M Zenke",
note = "Keywords: Base Sequence; Binding Sites; Blotting, Northern; Carbonic Anhydrases; Cell Differentiation; Cell Line, Transformed; Drug Resistance; Erythroblasts; Gene Expression Regulation; Genes, gag; Hela Cells; Humans; Luciferases; Molecular Sequence Data; Neomycin; Oncogene Proteins v-erbA; Plasmids; Precipitin Tests; Proto-Oncogene Proteins; Receptors, Thyroid Hormone; Retroviridae Proteins, Oncogenic; Triiodothyronine; Vaccinia virus",
year = "1991",
language = "English",
volume = "5",
pages = "2033--47",
journal = "Genes \& Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "11",
}