Validation of the AmpFℓSTR® SEfiler Plus™ PCR Amplification kit for forensic STR analysis

Stine Frisk Fredslund; Helle Smidt Mogensen; Niels Morling

doi:10.1016/j.fsigss.2009.09.014

Validation of the AmpFℓSTR^® SEfiler Plus™ PCR Amplification kit for forensic STR analysis

Stine Frisk Fredslund, Helle Smidt Mogensen, Niels Morling^*

^*Corresponding author af dette arbejde

Afdeling for Retsgenetik

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

2 Citationer (Scopus)

Abstract

Validation of the AmpFℓSTR^® SEfiler Plus™ PCR Amplification kit with 29 and 30 PCR cycles for forensic STR analysis demonstrated that the kit had fewer artefacts than the AmpFℓSTR^® SGM Plus™ kit (28 PCR cycles). The SEfiler Plus kit was more sensitive and devoid of colour artefacts, but showed more stutters, drop-ins, drop-outs and allelic imbalances.

Originalsprog	Engelsk
Tidsskrift	Forensic Science International: Genetics. Supplement Series
Vol/bind	2
Udgave nummer	1
Sider (fra-til)	27-28
Antal sider	2
ISSN	1875-1768
DOI	https://doi.org/10.1016/j.fsigss.2009.09.014
Status	Udgivet - 2009

Adgang til dokumentet

10.1016/j.fsigss.2009.09.014

Citationsformater

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title = "Validation of the AmpFℓSTR{\textregistered} SEfiler Plus{\texttrademark} PCR Amplification kit for forensic STR analysis",

abstract = "Validation of the AmpFℓSTR{\textregistered} SEfiler Plus{\texttrademark} PCR Amplification kit with 29 and 30 PCR cycles for forensic STR analysis demonstrated that the kit had fewer artefacts than the AmpFℓSTR{\textregistered} SGM Plus{\texttrademark} kit (28 PCR cycles). The SEfiler Plus kit was more sensitive and devoid of colour artefacts, but showed more stutters, drop-ins, drop-outs and allelic imbalances.",

keywords = "AmpFℓSTR SEfiler Plus{\texttrademark} PCR Amplification kit, Forensic genetics, Short tandem repeats (STRs)",

author = "Fredslund, {Stine Frisk} and Mogensen, {Helle Smidt} and Niels Morling",

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AU - Morling, Niels

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AB - Validation of the AmpFℓSTR® SEfiler Plus™ PCR Amplification kit with 29 and 30 PCR cycles for forensic STR analysis demonstrated that the kit had fewer artefacts than the AmpFℓSTR® SGM Plus™ kit (28 PCR cycles). The SEfiler Plus kit was more sensitive and devoid of colour artefacts, but showed more stutters, drop-ins, drop-outs and allelic imbalances.

KW - AmpFℓSTR SEfiler Plus™ PCR Amplification kit

KW - Forensic genetics

KW - Short tandem repeats (STRs)

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DO - 10.1016/j.fsigss.2009.09.014

M3 - Journal article

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