Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Lung Cancer |
| Vol/bind | 66 |
| Udgave nummer | 3 |
| Sider (fra-til) | 314-8 |
| Antal sider | 5 |
| ISSN | 0169-5002 |
| DOI | |
| Status | Udgivet - 2009 |
Bibliografisk note
Keywords: Carcinoma, Non-Small-Cell Lung; Female; Follow-Up Studies; Gene Expression Profiling; Humans; Immunohistochemistry; Kaplan-Meiers Estimate; Lung; Lung Neoplasms; Male; Neoplasm Staging; Prognosis; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor Receptor-2Adgang til dokumentet
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I: Lung Cancer, Bind 66, Nr. 3, 2009, s. 314-8.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Vascular endothelial growth factor A and vascular endothelial growth factor receptor 2 expression in non-small cell lung cancer patients: relation to prognosis
AU - Bonnesen, Barbara
AU - Pappot, Helle
AU - Holmstav, Julie
AU - Skov, Birgit Guldhammer
N1 - Keywords: Carcinoma, Non-Small-Cell Lung; Female; Follow-Up Studies; Gene Expression Profiling; Humans; Immunohistochemistry; Kaplan-Meiers Estimate; Lung; Lung Neoplasms; Male; Neoplasm Staging; Prognosis; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factor Receptor-2
PY - 2009
Y1 - 2009
N2 - BACKGROUND: The majority of patients with non-small cell lung cancer (NSCLC) are diagnosed with advanced inoperable disease. While treatment with conventional chemotherapy has improved during the last decade the 5 years survival is still modest. Novel drugs, which selectively target aberrant elements in neoplastic cells and their microenvironment have recently been and are continuously developed including drugs inhibiting the angiogenic system. Angiogenic factor vascular endothelial growth factor (VEGF) and its receptor vascular endothelial growth factor receptor 2 (VEGFR2) seem to play key roles in tumour-induced angiogenesis. Previous studies have been inconclusive on the topic of a role for VEGF and its receptor as prognostic factors in NSCLC. METHODS: Paraffin-embedded histological material from 102 patients operated for NSCLC was included and a representative block with lung cancer tissue was selected from each patient for immunohistochemical studies. The sections were incubated with primary monoclonal antibodies to VEGF-A and VEGFR2. The expression of the immunohistochemical staining was assessed semi-quantitatively by estimating the percentage and the intensity of tumour cells stained on whole tumour slides. Kaplan-Meier survival curves were generated to evaluate the significance of immunohistochemical VEGF-A and VEGFR2 expression for the prognosis. RESULTS: VEGF-A and VEGFR2 expression was observed in the majority of NSCLC patients. VEGF-A expression showed a correlation to histological type with increased expression in adenocarcinomas as compared to squamous cell carcinomas. There was no statistically significant correlation between VEGF-A and VEGFR2 expression and age, gender or stage at diagnosis. Finally there was no relation between expression of VEGF-A and VEGFR2, nor an effect of high expression of both VEGF-A and VEGFR2 on survival. CONCLUSION: In conclusion VEGF-A and VEGFR2 are expressed in NSCLC, but the immunohistochemical expression of VEGF-A and VEGFR2 has no prognostic impact in NSCLC. We show that the histological subgroups of NSCLC express VEGF-A differently, with adenocarcinomas having the highest amount. Whether these markers might be useful as clinically reliable predictive markers remains to be solved.
AB - BACKGROUND: The majority of patients with non-small cell lung cancer (NSCLC) are diagnosed with advanced inoperable disease. While treatment with conventional chemotherapy has improved during the last decade the 5 years survival is still modest. Novel drugs, which selectively target aberrant elements in neoplastic cells and their microenvironment have recently been and are continuously developed including drugs inhibiting the angiogenic system. Angiogenic factor vascular endothelial growth factor (VEGF) and its receptor vascular endothelial growth factor receptor 2 (VEGFR2) seem to play key roles in tumour-induced angiogenesis. Previous studies have been inconclusive on the topic of a role for VEGF and its receptor as prognostic factors in NSCLC. METHODS: Paraffin-embedded histological material from 102 patients operated for NSCLC was included and a representative block with lung cancer tissue was selected from each patient for immunohistochemical studies. The sections were incubated with primary monoclonal antibodies to VEGF-A and VEGFR2. The expression of the immunohistochemical staining was assessed semi-quantitatively by estimating the percentage and the intensity of tumour cells stained on whole tumour slides. Kaplan-Meier survival curves were generated to evaluate the significance of immunohistochemical VEGF-A and VEGFR2 expression for the prognosis. RESULTS: VEGF-A and VEGFR2 expression was observed in the majority of NSCLC patients. VEGF-A expression showed a correlation to histological type with increased expression in adenocarcinomas as compared to squamous cell carcinomas. There was no statistically significant correlation between VEGF-A and VEGFR2 expression and age, gender or stage at diagnosis. Finally there was no relation between expression of VEGF-A and VEGFR2, nor an effect of high expression of both VEGF-A and VEGFR2 on survival. CONCLUSION: In conclusion VEGF-A and VEGFR2 are expressed in NSCLC, but the immunohistochemical expression of VEGF-A and VEGFR2 has no prognostic impact in NSCLC. We show that the histological subgroups of NSCLC express VEGF-A differently, with adenocarcinomas having the highest amount. Whether these markers might be useful as clinically reliable predictive markers remains to be solved.
U2 - 10.1016/j.lungcan.2009.02.013
DO - 10.1016/j.lungcan.2009.02.013
M3 - Journal article
C2 - 19324448
SN - 0169-5002
VL - 66
SP - 314
EP - 318
JO - Lung Cancer
JF - Lung Cancer
IS - 3
ER -