Vasopressin-dependent short-term regulation of aquaporin 4 expressed in Xenopus oocytes

H B Moeller; R A Fenton; T Zeuthen; N Macaulay

doi:10.1016/j.neuroscience.2009.09.072

Vasopressin-dependent short-term regulation of aquaporin 4 expressed in Xenopus oocytes

H B Moeller, R A Fenton, T Zeuthen, N Macaulay

Institut for Cellulær og Molekylær Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

56 Citationer (Scopus)

Abstract

Udgivelsesdato: 2009-Dec-29

Originalsprog	Engelsk
Tidsskrift	Neuroscience
Vol/bind	164
Udgave nummer	4
Sider (fra-til)	1674-84
Antal sider	10
ISSN	0306-4522
DOI	https://doi.org/10.1016/j.neuroscience.2009.09.072
Status	Udgivet - dec. 2009

Adgang til dokumentet

10.1016/j.neuroscience.2009.09.072

Citationsformater

@article{ef953ac0fc3e11de825d000ea68e967b,

title = "Vasopressin-dependent short-term regulation of aquaporin 4 expressed in Xenopus oocytes",

abstract = "Aquaporin 4 (AQP4) is abundantly expressed in the perivascular glial endfeet in the central nervous system (CNS), where it is involved in the exchange of fluids between blood and brain. At this location, AQP4 contributes to the formation and/or the absorption of the brain edema that may arise following pathologies such as brain injuries, brain tumours, and cerebral ischemia. As vasopressin and its G-protein-coupled receptor (V1(a)R) have been shown to affect the outcome of brain edema, we have investigated the regulatory interaction between AQP4 and V1(a)R by heterologous expression in Xenopus laevis oocytes. The water permeability of AQP4/V1(a)R-expressing oocytes was reduced in a vasopressin-dependent manner, as a result of V1(a)R-dependent internalization of AQP4. Vasopressin-dependent internalization was not observed in AQP9/V1(a)R-expressing oocytes. The regulatory interaction between AQP4 and V1(a)R involves protein kinase C (PKC) activation and is reduced upon mutation of Ser(180) on AQP4 to an alanine. Thus, the present study demonstrates at the molecular level a functional link between the vasopressin receptor V1(a)R and AQP4. This functional interaction between AQP4 and V1(a)R may prove to be a potential therapeutic target in the prevention and treatment of brain edema.",

author = "Moeller, {H B} and Fenton, {R A} and T Zeuthen and N Macaulay",

year = "2009",

month = dec,

doi = "10.1016/j.neuroscience.2009.09.072",

language = "English",

volume = "164",

pages = "1674--84",

journal = "Neuroscience",

issn = "0306-4522",

publisher = "Pergamon Press",

number = "4",

}

TY - JOUR

T1 - Vasopressin-dependent short-term regulation of aquaporin 4 expressed in Xenopus oocytes

AU - Moeller, H B

AU - Fenton, R A

AU - Zeuthen, T

AU - Macaulay, N

PY - 2009/12

Y1 - 2009/12

N2 - Aquaporin 4 (AQP4) is abundantly expressed in the perivascular glial endfeet in the central nervous system (CNS), where it is involved in the exchange of fluids between blood and brain. At this location, AQP4 contributes to the formation and/or the absorption of the brain edema that may arise following pathologies such as brain injuries, brain tumours, and cerebral ischemia. As vasopressin and its G-protein-coupled receptor (V1(a)R) have been shown to affect the outcome of brain edema, we have investigated the regulatory interaction between AQP4 and V1(a)R by heterologous expression in Xenopus laevis oocytes. The water permeability of AQP4/V1(a)R-expressing oocytes was reduced in a vasopressin-dependent manner, as a result of V1(a)R-dependent internalization of AQP4. Vasopressin-dependent internalization was not observed in AQP9/V1(a)R-expressing oocytes. The regulatory interaction between AQP4 and V1(a)R involves protein kinase C (PKC) activation and is reduced upon mutation of Ser(180) on AQP4 to an alanine. Thus, the present study demonstrates at the molecular level a functional link between the vasopressin receptor V1(a)R and AQP4. This functional interaction between AQP4 and V1(a)R may prove to be a potential therapeutic target in the prevention and treatment of brain edema.

AB - Aquaporin 4 (AQP4) is abundantly expressed in the perivascular glial endfeet in the central nervous system (CNS), where it is involved in the exchange of fluids between blood and brain. At this location, AQP4 contributes to the formation and/or the absorption of the brain edema that may arise following pathologies such as brain injuries, brain tumours, and cerebral ischemia. As vasopressin and its G-protein-coupled receptor (V1(a)R) have been shown to affect the outcome of brain edema, we have investigated the regulatory interaction between AQP4 and V1(a)R by heterologous expression in Xenopus laevis oocytes. The water permeability of AQP4/V1(a)R-expressing oocytes was reduced in a vasopressin-dependent manner, as a result of V1(a)R-dependent internalization of AQP4. Vasopressin-dependent internalization was not observed in AQP9/V1(a)R-expressing oocytes. The regulatory interaction between AQP4 and V1(a)R involves protein kinase C (PKC) activation and is reduced upon mutation of Ser(180) on AQP4 to an alanine. Thus, the present study demonstrates at the molecular level a functional link between the vasopressin receptor V1(a)R and AQP4. This functional interaction between AQP4 and V1(a)R may prove to be a potential therapeutic target in the prevention and treatment of brain edema.

U2 - 10.1016/j.neuroscience.2009.09.072

DO - 10.1016/j.neuroscience.2009.09.072

M3 - Journal article

C2 - 19800950

SN - 0306-4522

VL - 164

SP - 1674

EP - 1684

JO - Neuroscience

JF - Neuroscience

IS - 4

ER -