Weaver mutant mouse cerebellar granule cells respond normally to chronic depolarization

Annette Bjerregaard; Helle Smidt Mogensen; N Hack; R Balázs; Ole Steen Jørgensen

Weaver mutant mouse cerebellar granule cells respond normally to chronic depolarization

Annette Bjerregaard, Helle Smidt Mogensen, N Hack, R Balázs, Ole Steen Jørgensen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

1 Citationer (Scopus)

Abstract

Udgivelsesdato: 1997-Apr

Originalsprog	Engelsk
Tidsskrift	International Journal of Developmental Neuroscience
Vol/bind	15
Udgave nummer	2
Sider (fra-til)	155-62
Antal sider	7
ISSN	0736-5748
Status	Udgivet - 1997

Bibliografisk note

Keywords: Animals; Cell Aging; Cell Differentiation; Cell Survival; Cells, Cultured; Cerebellum; Deoxyglucose; Electrophysiology; Mice; Mice, Neurologic Mutants; N-Methylaspartate; Neural Cell Adhesion Molecules; Potassium; Reference Values; Time Factors

Citationsformater

@article{5aaea66074cb11dbbee902004c4f4f50,

title = "Weaver mutant mouse cerebellar granule cells respond normally to chronic depolarization",

abstract = "We studied the effects of chronic K(+)-induced membrane depolarization and treatment with N-methyl-D-aspartate (NMDA) on cerebellar granule cells (CGCs) from weaver mutant mice and non-weaver litter-mates. The weaver mutation is a Gly-to-Ser substitution in a conserved region of the Girk2 G protein-coupled inward rectifying potassium channel [Patil N., Cox D. R., Bhat D., Faham M., Myers R. M. and Peterson A. S. (1995) Nature Genet. 11, 126-129] which induces early death of CGCs. The biochemical differentiation of CGCs was estimated as the rate of 2-deoxy-D-glucose accumulation and the expression of neural cell adhesion molecule (NCAM). High (25 mM) K+ ion concentration or treatment with NMDA greatly promoted the biochemical differentiation of both weaver mutant and non-weaver litter-mate mouse CGCs. In contrast to the marked effect on biochemical differentiation in both weaver and non-weaver mice CGSs, chronic high K+ treatment only had limited effect on survival. The survival of weaver mutant mouse CGCs in medium containing 5 mM K+ ions was very low, only 20% of the plated cells surviving at 7 days after plating, as opposed to the 50% for non-weaver CGCs. Chronic high K+ treatment improved the relative survival of weaver mutant mouse CGCs 1.6 2.2-fold and that of non-weaver CGCs 1.2-1.4-fold; the same number of CGCs (about 20% of the plated cells) were rescued by high K+ in both types of culture. The findings indicate that, in culture weaver mutant mouse, CGCs have a normal response to membrane depolarization and that the normal function of the Girk2 potassium channel is not critical for the survival of differentiated CGCs.",

author = "Annette Bjerregaard and Mogensen, {Helle Smidt} and N Hack and R Bal{\'a}zs and J{\o}rgensen, {Ole Steen}",

note = "Keywords: Animals; Cell Aging; Cell Differentiation; Cell Survival; Cells, Cultured; Cerebellum; Deoxyglucose; Electrophysiology; Mice; Mice, Neurologic Mutants; N-Methylaspartate; Neural Cell Adhesion Molecules; Potassium; Reference Values; Time Factors",

year = "1997",

language = "English",

volume = "15",

pages = "155--62",

journal = "International Journal of Developmental Neuroscience",

issn = "0736-5748",

publisher = "Pergamon Press",

number = "2",

}

TY - JOUR

T1 - Weaver mutant mouse cerebellar granule cells respond normally to chronic depolarization

AU - Bjerregaard, Annette

AU - Mogensen, Helle Smidt

AU - Hack, N

AU - Balázs, R

AU - Jørgensen, Ole Steen

N1 - Keywords: Animals; Cell Aging; Cell Differentiation; Cell Survival; Cells, Cultured; Cerebellum; Deoxyglucose; Electrophysiology; Mice; Mice, Neurologic Mutants; N-Methylaspartate; Neural Cell Adhesion Molecules; Potassium; Reference Values; Time Factors

PY - 1997

Y1 - 1997

N2 - We studied the effects of chronic K(+)-induced membrane depolarization and treatment with N-methyl-D-aspartate (NMDA) on cerebellar granule cells (CGCs) from weaver mutant mice and non-weaver litter-mates. The weaver mutation is a Gly-to-Ser substitution in a conserved region of the Girk2 G protein-coupled inward rectifying potassium channel [Patil N., Cox D. R., Bhat D., Faham M., Myers R. M. and Peterson A. S. (1995) Nature Genet. 11, 126-129] which induces early death of CGCs. The biochemical differentiation of CGCs was estimated as the rate of 2-deoxy-D-glucose accumulation and the expression of neural cell adhesion molecule (NCAM). High (25 mM) K+ ion concentration or treatment with NMDA greatly promoted the biochemical differentiation of both weaver mutant and non-weaver litter-mate mouse CGCs. In contrast to the marked effect on biochemical differentiation in both weaver and non-weaver mice CGSs, chronic high K+ treatment only had limited effect on survival. The survival of weaver mutant mouse CGCs in medium containing 5 mM K+ ions was very low, only 20% of the plated cells surviving at 7 days after plating, as opposed to the 50% for non-weaver CGCs. Chronic high K+ treatment improved the relative survival of weaver mutant mouse CGCs 1.6 2.2-fold and that of non-weaver CGCs 1.2-1.4-fold; the same number of CGCs (about 20% of the plated cells) were rescued by high K+ in both types of culture. The findings indicate that, in culture weaver mutant mouse, CGCs have a normal response to membrane depolarization and that the normal function of the Girk2 potassium channel is not critical for the survival of differentiated CGCs.

AB - We studied the effects of chronic K(+)-induced membrane depolarization and treatment with N-methyl-D-aspartate (NMDA) on cerebellar granule cells (CGCs) from weaver mutant mice and non-weaver litter-mates. The weaver mutation is a Gly-to-Ser substitution in a conserved region of the Girk2 G protein-coupled inward rectifying potassium channel [Patil N., Cox D. R., Bhat D., Faham M., Myers R. M. and Peterson A. S. (1995) Nature Genet. 11, 126-129] which induces early death of CGCs. The biochemical differentiation of CGCs was estimated as the rate of 2-deoxy-D-glucose accumulation and the expression of neural cell adhesion molecule (NCAM). High (25 mM) K+ ion concentration or treatment with NMDA greatly promoted the biochemical differentiation of both weaver mutant and non-weaver litter-mate mouse CGCs. In contrast to the marked effect on biochemical differentiation in both weaver and non-weaver mice CGSs, chronic high K+ treatment only had limited effect on survival. The survival of weaver mutant mouse CGCs in medium containing 5 mM K+ ions was very low, only 20% of the plated cells surviving at 7 days after plating, as opposed to the 50% for non-weaver CGCs. Chronic high K+ treatment improved the relative survival of weaver mutant mouse CGCs 1.6 2.2-fold and that of non-weaver CGCs 1.2-1.4-fold; the same number of CGCs (about 20% of the plated cells) were rescued by high K+ in both types of culture. The findings indicate that, in culture weaver mutant mouse, CGCs have a normal response to membrane depolarization and that the normal function of the Girk2 potassium channel is not critical for the survival of differentiated CGCs.

M3 - Journal article

C2 - 9178034

VL - 15

SP - 155

EP - 162

JO - International Journal of Developmental Neuroscience

JF - International Journal of Developmental Neuroscience

SN - 0736-5748

IS - 2

ER -