Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis

Bo G Winkel; Mads Vilhelm Hollegaard; Morten S Olesen; Jesper H Svendsen; Stig Haunsø; David M Hougaard; Jacob Tfelt-Hansen

doi:10.1186/1471-2350-12-22

Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis

Bo G Winkel, Mads Vilhelm Hollegaard, Morten S Olesen, Jesper H Svendsen, Stig Haunsø, David M Hougaard, Jacob Tfelt-Hansen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

22 Citationer (Scopus)

Abstract

The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples, constitutes a reliable alternative to gDNA.We wanted to compare melting curves and sequencing results from wgaDNA derived from DBS samples with gDNA derived from whole blood.

Originalsprog	Engelsk
Tidsskrift	B M C Medical Genetics
Vol/bind	12
Sider (fra-til)	22
ISSN	1471-2350
DOI	https://doi.org/10.1186/1471-2350-12-22
Status	Udgivet - 2011

Adgang til dokumentet

10.1186/1471-2350-12-22

Citationsformater

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title = "Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis",

abstract = "The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples, constitutes a reliable alternative to gDNA.We wanted to compare melting curves and sequencing results from wgaDNA derived from DBS samples with gDNA derived from whole blood.",

author = "Winkel, {Bo G} and Hollegaard, {Mads Vilhelm} and Olesen, {Morten S} and Svendsen, {Jesper H} and Stig Hauns{\o} and Hougaard, {David M} and Jacob Tfelt-Hansen",

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doi = "10.1186/1471-2350-12-22",

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T1 - Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis

AU - Winkel, Bo G

AU - Hollegaard, Mads Vilhelm

AU - Olesen, Morten S

AU - Svendsen, Jesper H

AU - Haunsø, Stig

AU - Hougaard, David M

AU - Tfelt-Hansen, Jacob

PY - 2011

Y1 - 2011

N2 - The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples, constitutes a reliable alternative to gDNA.We wanted to compare melting curves and sequencing results from wgaDNA derived from DBS samples with gDNA derived from whole blood.

AB - The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples, constitutes a reliable alternative to gDNA.We wanted to compare melting curves and sequencing results from wgaDNA derived from DBS samples with gDNA derived from whole blood.

U2 - 10.1186/1471-2350-12-22

DO - 10.1186/1471-2350-12-22

M3 - Journal article

SN - 1471-2350

VL - 12

SP - 22

JO - B M C Medical Genetics

JF - B M C Medical Genetics

ER -