Frederik Ravnkilde Marlet
  • Jagtvej 162

    2100 København Ø

  • Universitetsparken 2

    2100 København Ø

  • Source: Scopus
20212024

Research activity per year

Personal profile

Short presentation

Identification of lipid changes associated with Parkinson’s Disease and alpha-synuclein pathology in dopaminergic neuronal cells.
 

My current focuses are:

1. Development of novel PD model by using the human derived neuroblastoma cell line, SH-SY5Y. The aim is to investigate the lipid composition in both the healthy and diseased model. PD-like symptoms are established in the SH-SY5Y cells by GBA knockout and oxidative stress repectively.

2. Investigate the affect of lipid vesicles on a-synuclein aggregation. The vesicles are prepared from various sphingolipids differing in size and side chains bound to the sphingosine backbone. These lipids are proven to be the most abundant lipids in the brain and the levels of these indivdual lipids are perturbed in PD.

3. Identification of fibril characteristics by electron microscopy. Fibrils formed by aggregation of a-synuclein in presence of lipid vesicles prepared from different sphingolipids, are used. 

4. Investigation if Ambroxol can restore the effects on the whole cell lipid profile generated from expression of mutated GCase. Patient-derived fibroblasts expressing mutated GCase will be used as  model.
 

Methods:

- Protein expression and purification
- Tht aggregation experiment
- Protein-lipid binding assay (Circular dichroism)
- Preparation of lipid vesicles
- Fibril formation
- Electron microscopy
- Cell culturing
- Lipid extraction
- Lipidomics
- Gcase activity assay
- Lysosome isolation
- qPCR
- Introduction of oxidative stress (w. MPP+ and 6-OHDA)
- MTT viability assay

CV

Education:

2019: Pharmacy, Masters degree, University of Copenhagen

2016: Pharmacy, Bachelors degree, University of Copenhagen

 

Recent positions: 

2019: PhD fellow (current position)
Peptides and Proteins
University of Copenhagen
Copenhagen Ø

2019: Guest researcher (Current position)
Danish Centre for Cancer Research, Copenhagen, Denmark

2019: Chemical assessor
Danish Medicines Agency
Copenhagen S, Denmark 

2017: Guest researcher (student)
University of Otago, Dunedin
New Zealand
 

Teaching

2020: SFABIL107U - Cellular and molecular biology (Bachelor in Pharmacy)

2019: SFABIL107U - Cellular and molecular biology (Bachelor in Pharmacy)

 

Education/Academic qualification

Pharmacy, Masters degree, University of Copenhagen

1 Sep 201631 Mar 2019

Award Date: 31 Mar 2019

Pharmacy, Bachelors degree, University of Copenhagen

1 Sep 201330 Jun 2016

Award Date: 30 Jun 2016

External positions

Guest Researcher, Danish Cancer Society

1 Dec 201930 Nov 2021

Chemical assessor, Lægemiddelstyrelsen

1 Jul 201930 Sep 2019

Guest reasearcher, University of Otago

1 Jul 201731 Dec 2017

Collaborations and top research areas from the last five years

Recent external collaboration on country/territory level. Dive into details by clicking on the dots or