Research output per year
Research output per year
Jagtvej 162
2100 København Ø
Universitetsparken 2
2100 København Ø
Research activity per year
Identification of lipid changes associated with Parkinson’s Disease and alpha-synuclein pathology in dopaminergic neuronal cells.
My current focuses are:
1. Development of novel PD model by using the human derived neuroblastoma cell line, SH-SY5Y. The aim is to investigate the lipid composition in both the healthy and diseased model. PD-like symptoms are established in the SH-SY5Y cells by GBA knockout and oxidative stress repectively.
2. Investigate the affect of lipid vesicles on a-synuclein aggregation. The vesicles are prepared from various sphingolipids differing in size and side chains bound to the sphingosine backbone. These lipids are proven to be the most abundant lipids in the brain and the levels of these indivdual lipids are perturbed in PD.
3. Identification of fibril characteristics by electron microscopy. Fibrils formed by aggregation of a-synuclein in presence of lipid vesicles prepared from different sphingolipids, are used.
4. Investigation if Ambroxol can restore the effects on the whole cell lipid profile generated from expression of mutated GCase. Patient-derived fibroblasts expressing mutated GCase will be used as model.
Methods:
- Protein expression and purification
- Tht aggregation experiment
- Protein-lipid binding assay (Circular dichroism)
- Preparation of lipid vesicles
- Fibril formation
- Electron microscopy
- Cell culturing
- Lipid extraction
- Lipidomics
- Gcase activity assay
- Lysosome isolation
- qPCR
- Introduction of oxidative stress (w. MPP+ and 6-OHDA)
- MTT viability assay
2019: Pharmacy, Masters degree, University of Copenhagen
2016: Pharmacy, Bachelors degree, University of Copenhagen
2019: PhD fellow (current position)
Peptides and Proteins
University of Copenhagen
Copenhagen Ø
2019: Guest researcher (Current position)
Danish Centre for Cancer Research, Copenhagen, Denmark
2019: Chemical assessor
Danish Medicines Agency
Copenhagen S, Denmark
2020: SFABIL107U - Cellular and molecular biology (Bachelor in Pharmacy)
2019: SFABIL107U - Cellular and molecular biology (Bachelor in Pharmacy)
Pharmacy, Masters degree, University of Copenhagen
1 Sep 2016 → 31 Mar 2019
Award Date: 31 Mar 2019
Pharmacy, Bachelors degree, University of Copenhagen
1 Sep 2013 → 30 Jun 2016
Award Date: 30 Jun 2016
Guest Researcher, Danish Cancer Society
1 Dec 2019 → 30 Nov 2021
Chemical assessor, Lægemiddelstyrelsen
1 Jul 2019 → 30 Sep 2019
Guest reasearcher, University of Otago
1 Jul 2017 → 31 Dec 2017
Research output: Contribution to journal › Journal article › Research › peer-review
Research output: Contribution to journal › Journal article › Research › peer-review
Research output: Contribution to journal › Journal article › Research › peer-review
Research output: Contribution to journal › Journal article › Research › peer-review
Research output: Contribution to journal › Review › Research › peer-review
Marlet, Frederik Ravnkilde (Recipient), 26 Aug 2022
Prize: Prizes, scholarships, distinctions
Marlet, Frederik Ravnkilde (Recipient), Mar 2022
Prize: Prizes, scholarships, distinctions