CV

Curriculum vitae

Name: Hans Jakob Larsen

Born: 4. januar 1966

Place of birth: Nuuk, Greenland

Citizenship: Danish

Marital status: Married, three children

Education

1992 Master of Science (cand. scient.), University of Aarhus

1994 Ph.D, University of Aarhus

1999 Ten days course at Forensic Science Service, Metropolitan Police, London, UK

2002 Two days course on "Oracle" (SQL for end-users)

2005 Two days course on "Forensic Statistics" by Bruce S Weir

2006 Certification Course in Thomas Person Profile Analysis (Thomas International)

Leisure interests

Fishing, boating, camping, skiing, scuba diving

Forensic experience

1999-2009 Ten years of crime case work (Section for Crime Cases, Department of Forensic Genetics, Institute of Forensic Medicine, University of Copenhagen)

2005 Two periods of three weeks in Phuket, Thailand assisting the Thai Tsunami Victim Identification operation

2003-2009 Regularly giving evidence as an expert witness in court (approx. 30 times)

Management experience

Lab manager or assistant lab manager for a lab (5 - 16 technicians) examining items and samples for biological traces (7 years)

Temporary manager of the Crime Case Section (~20 academics and ~40 technicians) of the Department of Forensic Genetics (4 months)

Teaching and talks

1990 "Genetics" A  course for biology students (University of Aarhus, Faculty of Natural Science)

1992 "Transgenic Animals" a course for biology students (University of Aarhus, Faculty of Natural Science) in cooperation with cand. scient. Thomas Mikkelsen

1996-1997 "Cell biology" for medical students (University of Copenhagen, Faculty of Health Sciences)

1998 The EMBO course "Probing of nucleic acids by metal-ion complexes" for Danish and international Ph.D. students (University of Copenhagen, Faculty of Health Sciences)

2001- "Examination of biological trace material" for staff from the National Danish Police, Technical Department. (University of Copenhagen, Faculty of Health Sciences).

2002- "Advanced investigation", the national Danish police school (approx. 3 times per year)

2008  "The Genetic Identity" a talk open to the public

2008 "Meet the Science" a talk open to the public

2008 - "The statistical weight of DNA evidence", a seminar for Danish judges, prosecutors, and defense attorneys.

2009- "Crime Scene Investigation and DNA-analysis", a course for Danish defense attorneys.

Positions

1990 Assistant teacher "Genetics" for biology-students, (University of Aarhus, Faculty of Natural Science)

1991 Scholarship financed by the Research Foundation of University of Aarhus) (Institute of Molecular and Structural Biology, University of Aarhus Universitet)

1992-1994 Postgraduate scholarship financed by the Research Foundation of University of Aarhus. (Institute of Molecular and Structural Biology, University of Aarhus Universitet)

1994-1999 Postdoctoral Research Fellow financed by The Danish National Research Foundation (Center for Biomolecular Recognition, Institute of Medical Biochemistry of Genetics, University of Copenhagen)

1999- Forensic Geneticist (Section for Crime Cases, Department of Forensic Genetics, Institute of Forensic Medicine, University of Copenhagen)

Molecular Techniques

Cloning: Plasmidcloning, M13 phagecloning, construction of ¿-libraries.

DNA-preparation: Plasmid DNA, genomic DNA, ¿ -DNA, cosmid-DNA, ssM13 DNA. DNA-extraction from biological tracematerial.

DNA-analysis: Screening of ¿ -libraries and isolation of genes, Southern-analysis of plasmid- and genomic DNA, RFLP, PCR, restrictionsmapping of genomic and cloned DNA, sequencing (chemical and enzymatic), multiplex-PCR-based STR analysis (SGM+, Identifiler), sequencing of mitochondrial DNA.

RNA-analysis: In vitro transcription, isolation of RNA, Northern blot analysis, primer extension/cDNA-synthesis.

Analysis of DNA-structure and complexes involving DNA: Electroforetic mobility-shift assay, footprinting using enzymatic and chemical probes (DNase, KMnO4, dimethyl sulfate, diethyl pyrocarbonat, Fe-EDTA).

Protein-analysis: SDS-polyacrylamid geleletroforesis, rocket elektroforesis, Western blot, iso-electric focusing, 2-D gelelectroforesis.

Scientific publications:

• Kaarstad K., Rohde M., Larsen, J., Eriksen, B, Thomsen J.L.: The detection of female DNA from the penis in sexual assault cases. J Forensic and Legal Medicine.14 p 159-160. 2007

• Bentin T., Cherny D., Larsen, H.J., Nielsen P.E.: Transcription arrest caused by long nascent RNA chains. Biochemica et Biophysica Acta. 1727, p. 97-105. 2005.

• Bentin, T., Larsen, H.J., Nielsen P.E.: Peptide Nucleic Acid targeting of double-stranded dna. i: Peptide Nucleic Acids: Protocols and Applications p. 107-140. Horizon Bioscience Press, Norfolk, England 2004.

• Rasmussen E.M., Sørensen E., Eriksen, B., Larsen, H.J., Morling, N.: Mitochondrial dna polymorphism in 103 unrelated Caucasian Danes. Progress in Forensic Genetics 10. ICS International Congress Series 1261, p. 401-403. 2004.

• Sørensen E., Rasmussen E.M., Eriksen, B., Larsen, H.J., Morling, N.: Evaluation and validation of quality assessment of mitochondrial control region sequence data by Phred. Progress in Forensic Genetics 10. ICS International Congress Series 1261, p. 94-96. 2004.

• Bentin T., Larsen, H.J., Nielsen P.E.: Combined triplex/duplex invasion of double-standed dna by "tail-clamp" peptide nucleic acid. Biochemistry 42, p. 13987-13995. 2003.

• Rasmussen E.M., Sørensen E., Eriksen, B., Larsen, H.J., Morling, N.: Sequencing strategy of mitochondrial HV1 and HV2 DNA with length heteroplasmy. Forensic Science International 129, p. 209-213. 2002.

• Hansen G.I., Bentin T., Larsen, H.J., Nielsen, P.E.: Structural isomers of bis-PNA bound to a target in duplex DNA. J.Mol.Bio. Vol.307, p. 67-74. 2001.

• Larsen, H.J., Bentin T., Nielsen, P.E.: Antisense properties of peptide nucleic acid. BBA 1489, p. 159-166. 1999.

• Larsen, H.J., Nielsen, P.E.: Characterization of PNA-dsDNA strand displacement, complexes, kap. 5.2. i: Peptide nucleic acids: Protocols and applications s. 221-240. Nielsen, Peter E. and Egholm, Michael. Horizon scientific press, Wymondham, UK 1999.

• Kurakin A., Larsen, H.J., Nielsen, P.E.: Cooperative strand displacement by peptide nucleic acid (PNA). Chemistry & Biology 5, 2, s. 81-89. 1998.

• Laursen, J., Larsen, H. J., Mikkelsen, T.R., Hjorth, J. P. Allele-specific expression of the PSP gene in the mouse sublingual glands Genet Res 1998 71 187-91. (1998).

• Larsen, H.J., Nielsen, P.E. Transcription Mediated Binding of Peptide Nucleic Acid (PNA) to Double-Stranded DNA: Sequence-Specific Suicide Transcription. Nucleic Acids Research 24 458-463 (1996).

• Larsen, H.J. Ph.D. thesis. Identification of Psp Regulatory Sequences and their Use for Expressing Heterologous Proteins in the Saliva of Transgenic Mice (1994).

• Larsen, H.J, Brodersen, C.and J. Peter Hjorth High Level Expression in The Salivary Glands of Transgenic Mice Transgenic Research 3 315-316 (1994).

• Larsen, H.J. Thesis The Design and Applications of a Gene Construct for Expression and Direction of Heterologous Peptides in the Saliva of Transgenic Mice (1992).

• Mikkelsen, T.R, Jakob Brandt, H. Jakob Larsen, Birte B. Larsen, Knud Poulsen, Jørgen Ingerslev, Nanni Din, and J. Peter Hjorth. Tissue Specific Expression in the Salivary Glands of Transgenic Mice Nucleic Acid Research, 20 2249-2255 (1992).