Jens Bukh

Search results

• 2022

  Two-component vaccine consisting of virus-like particles displaying hepatitis C virus envelope protein 2 oligomers

  Prentoe, J., Janitzek, C. M., Velázquez-Moctezuma, R., Soerensen, A., Jørgensen, T., Clemmensen, S., Soroka, V., Thrane, S., Theander, T., Nielsen, M. A., Salanti, A., Bukh, J. & Sander, A. F., 2022, In: npj Vaccines. 7, 148.

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  6 Citations (Scopus)

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• Versatile SARS-CoV-2 Reverse-Genetics Systems for the Study of Antiviral Resistance and Replication

  Fahnøe, U., Pham, L. V., Fernandez-Antunez, C., Costa, R., Rivera-Rangel, L. R., Galli, A., Feng, S., Mikkelsen, L. S., Gottwein, J. M., Scheel, T. K. H., Ramirez, S. & Bukh, J., 2022, In: Viruses. 14, 2, 172.

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  17 Citations (Scopus)

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• 2021

  Antigenic and immunogenic evaluation of permutations of soluble hepatitis C virus envelope protein E2 and E1 antigens

  Prentoe, J., Janitzek, C. M., Velázquez-Moctezuma, R., Goksøyr, L., Olsen, R. W., Fanalista, M., Augestad, E. H., Thrane, S., Pihl, A. F., Gottwein, J. M., Sander, A. F. & Bukh, J., 2021, In: PLoS ONE. 16, e0255336.

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  2 Citations (Scopus)

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• Characterization of a novel hepatitis C virus genotype 1 subtype from a patient failing 4 weeks of glecaprevir-pibrentasvir treatment

  Pedersen, M. S., Fahnøe, U., Madsen, L. W., Christensen, P. B., Øvrehus, A. & Bukh, J., 2021, In: Microbiology resource announcements. 10, 41, e00755.

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  3 Citations (Scopus)

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• Efficacy of ion-channel inhibitors amantadine, memantine and rimantadine for the treatment of SARS-CoV-2 in vitro

  Zhou, Y., Gammeltoft, K. A., Galli, A., Offersgaard, A., Fahnøe, U., Ramirez, S., Bukh, J. & Gottwein, J. M., 2021, In: Viruses. 13, 10, 2082.

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  23 Citations (Scopus)

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• Functional convergence of a germline-encoded neutralizing antibody response in rhesus macaques immunized with HCV envelope glycoproteins

  Chen, F., Tzarum, N., Lin, X., Giang, E., Velázquez-Moctezuma, R., Augestad, E. H., Nagy, K., He, L., Hernandez, M., Fouch, M. E., Grinyó, A., Chavez, D., Doranz, B. J., Prentoe, J., Stanfield, R. L., Lanford, R., Bukh, J., Wilson, I. A., Zhu, J. & Law, M., 2021, In: Immunity. 54, 4, p. 781-796.e4

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  23 Citations (Scopus)

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• Global evolutionary analysis of chronic hepatitis C patients revealed significant effect of baseline viral resistance, including novel non-target sites, for DAA-based treatment and retreatment outcome

  Fahnøe, U., Pedersen, M. S., Sølund, C., Ernst, A., Krarup, H. B., Røge, B. T., Christensen, P. B., Laursen, A. L., Gerstoft, J., Thielsen, P., Madsen, L. G., Pedersen, A. G., Schønning, K., Weis, N. & Bukh, J., 2021, In: Journal of Viral Hepatitis. 28, 2, p. 302-316

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  8 Citations (Scopus)

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• Hepatitis C virus envelope protein dynamics and the link to hypervariable region 1

  Augestad, E. H., Bukh, J. & Prentoe, J., 2021, In: Current Opinion in Virology. 50, p. 69-75 7 p.

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  8 Citations (Scopus)

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• Hepatitis C Virus Protease Inhibitors Show Differential Efficacy and Interactions with Remdesivir for Treatment of SARS-CoV-2 in Vitro

  Gammeltoft, K. A., Zhou, Y., Duarte Hernandez, C. R., Galli, A., Offersgaard, A., Costa, R., Pham, L. V., Fahnøe, U., Feng, S., Scheel, T. K. H., Ramirez, S., Bukh, J. & Gottwein, J. M., 2021, In: Antimicrobial Agents and Chemotherapy. 65, 9, e02680-20.

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  31 Citations (Scopus)

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• Inferior cure rate in pilot study of 4-week glecaprevir/pibrentasvir treatment with or without ribavirin of chronic hepatitis C

  Madsen, L. W., Christensen, P. B., Fahnøe, U., Pedersen, M. S., Bukh, J. & Øvrehus, A., 2021, In: Liver International. 41, 11, p. 2601-2610

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  10 Citations (Scopus)

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• In vitro adaptation and characterization of attenuated hypervariable region 1 swap chimeras of hepatitis C virus

  Olesen, C. H., Augestad, E. H., Troise, F., Bukh, J. & Prentoe, J., 2021, In: PLOS Pathogens. 17, July, 29 p., e1009720.

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  5 Citations (Scopus)

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• In vitro Characterization of Fitness and Convalescent Antibody Neutralization of SARS-CoV-2 Cluster 5 Variant Emerging in Mink at Danish Farms

  Lassaunière, R., Fonager, J., Rasmussen, M., Frische, A., Polacek, C., Rasmussen, T. B., Lohse, L., Belsham, G. J., Underwood, A., Winckelmann, A. A., Bollerup, S., Bukh, J., Weis, N., Sækmose, S. G., Aagaard, B., Alfaro-Núñez, A., Mølbak, K., Bøtner, A. & Fomsgaard, A., 2021, In: Frontiers in Microbiology. 12, 9 p., 698944.

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  37 Citations (Scopus)

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• In vitro efficacy of artemisinin-based treatments against SARS-CoV-2

  Zhou, Y., Gilmore, K., Ramirez, S., Settels, E., Gammeltoft, K. A., Pham, L. V., Fahnøe, U., Feng, S., Offersgaard, A., Trimpert, J., Bukh, J., Osterrieder, K., Gottwein, J. M. & Seeberger, P. H., 2021, In: Scientific Reports. 11, 14571.

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  71 Citations (Scopus)

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• Lipid Droplets Accumulation during Hepatitis C Virus Infection in Cell-Culture Varies among Genotype 1-3 Strains and Does Not Correlate with Virus Replication

  Galli, A., Ramirez, S. & Bukh, J., 2021, In: Viruses. 13, 3, 389.

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  9 Citations (Scopus)

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• Neutralisation titres against SARS-CoV-2 are sustained 6 months after onset of symptoms in individuals with mild COVID-19

  Underwood, A. P., Sølund, C., Fernandez-Antunez, C., Villadsen, S. L., Winckelmann, A. A., Bollerup, S., Mikkelsen, L. S., Sørensen, A. L., Feng, S., Fahnøe, U., Lassauniere, R., Fomsgaard, A., Ramirez, S., Weis, N. & Bukh, J., 2021, In: EBioMedicine. 71, 103519.

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  17 Citations (Scopus)

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• Overcoming culture restriction for SARS-CoV-2 in human cells facilitates the screening of compounds inhibiting viral replication

  Ramirez, S., Fernandez-Antunez, C., Galli, A., Underwood, A., Pham, L. V., Ryberg, L. A., Feng, S., Pedersen, M. S., Mikkelsen, L. S., Belouzard, S., Dubuisson, J., Sølund, C., Weis, N., Gottwein, J. M., Fahnøe, U. & Bukh, J., 2021, In: Antimicrobial Agents and Chemotherapy. 65, 7, 20 p., e00097-21.

  Research output: Contribution to journal › Journal article › Research › peer-review

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  63 Citations (Scopus)

• Pathogenesis, miR-122 gene-regulation, and protective immune responses after acute equine hepacivirus infection

  Tomlinson, J. E., Wolfisberg, R., Fahnøe, U., Patel, R. S., Trivedi, S., Kumar, A., Sharma, H., Nielsen, L., McDonough, S. P., Bukh, J., Tennant, B. C., Kapoor, A., Rosenberg, B. R., Rice, C. M., Divers, T. J., Van de Walle, G. R. & Scheel, T. K. H., 2021, In: Hepatology. 74, 3, p. 1148-1163

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  16 Citations (Scopus)

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• Sars-cov-2 production in a scalable high cell density bioreactor

  Offersgaard, A., Hernandez, C. R. D., Pihl, A. F., Costa, R., Venkatesan, N. P., Lin, X., Van Pham, L., Feng, S., Fahnøe, U., Scheel, T. K. H., Ramirez, S., Reichl, U., Bukh, J., Genzel, Y. & Gottwein, J. M., 2021, In: Vaccines. 9, 7, 706.

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  19 Citations (Scopus)

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• Vaccines against hepatitis C: A travel into neutralisation space

  Bukh, J., 2021, In: Gut. 70, 9, p. 1609-1610 2 p., 323377.

  Research output: Contribution to journal › Comment/debate › peer-review
  3 Citations (Scopus)

• Viral genome wide association study identifies novel hepatitis C virus polymorphisms associated with sofosbuvir treatment failure

  Smith, D. A., Fernandez-Antunez, C., Magri, A., Bowden, R., Chaturvedi, N., Fellay, J., McLauchlan, J., Foster, G. R., Irving, W. L., Ball, J., Brainard, D., Burgess, G., Cooke, G., Dillon, J., Gore, C., Guha, N., Halford, R., Herath, C., Holmes, C., Howe, A. & 25 others, Hudson, E., Irving, W., Khakoo, S., Klenerman, P., Koletzki, D., Martin, N., Massetto, B., Mbisa, T., McHutchison, J., McKeating, J., Miners, A., Murray, A., Shaw, P., Spencer, C. C. A., Targett-Adams, P., Thomson, E., Vickerman, P., Zitzmann, N., Simmonds, P., Pedergnana, V., Ramirez, S., Bukh, J., Barnes, E., Ansari, M. A. & STOP-HCV Consortium, 2021, In: Nature Communications. 12, 1, 11 p., 6105.

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  12 Citations (Scopus)

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• 2020

  An alternate conformation of HCV E2 neutralizing face as an additional vaccine target

  Tzarum, N., Giang, E., Kadam, R. U., Chen, F., Nagy, K., Augestad, E. H., Velázquez-Moctezuma, R., Keck, Z. Y., Hua, Y., Stanfield, R. L., Dreux, M., Prentoe, J., Foung, S. K. H., Bukh, J., Wilson, I. A. & Law, M., 2020, In: Science Advances. 6, 30, abb5642.

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  25 Citations (Scopus)

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• Analysis of virus population profiles within pigs infected with virulent Classical Swine Fever viruses; evidence for bottlenecks in transmission but absence of tissue specific virus variants

  Johnston, C. M., Fahnøe, U., Lohse, L., Bukh, J., Belsham, G. J. & Rasmussen, T. B., 2020, In: Journal of Virology. 94, 19, 20 p., e01119-20.

  Research output: Contribution to journal › Journal article › Research › peer-review

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  2 Citations (Scopus)

• Antibody Responses to Immunization With HCV Envelope Glycoproteins as a Baseline for B-Cell–Based Vaccine Development

  Chen, F., Nagy, K., Chavez, D., Willis, S., McBride, R., Giang, E., Honda, A., Bukh, J., Ordoukhanian, P., Zhu, J., Frey, S., Lanford, R. & Law, M., 2020, In: Gastroenterology. 158, 4, p. 1058-1071.e6

  Research output: Contribution to journal › Journal article › Research › peer-review

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  36 Citations (Scopus)

• Cell culture studies of the efficacy and barrier to resistance of sofosbuvir-velpatasvir and glecaprevir-pibrentasvir against hepatitis C virus genotypes 2a, 2b, and 2C

  Ramirez, S., Fernandez-Antunez, C., Mikkelsen, L. S., Pedersen, J., Li, Y. P. & Bukh, J., 2020, In: Antimicrobial Agents and Chemotherapy. 64, 3, e01888.

  Research output: Contribution to journal › Journal article › Research › peer-review

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  15 Citations (Scopus)

• Development of a downstream process for the production of an inactivated whole hepatitis C virus vaccine

  Lothert, K., Offersgaard, A. F., Pihl, A. F., Mathiesen, C. K., Jensen, T. B., Alzua, G. P., Fahnøe, U., Bukh, J., Gottwein, J. M. & Wolff, M. W., 2020, In: Scientific Reports. 10, 13 p., 16261.

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  31 Citations (Scopus)

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• Efficient culture of SARS-CoV-2 in human hepatoma cells enhances viability of the virus in human lung cancer cell lines permitting the screening of antiviral compounds

  Ramirez, S., Fernandez-Antunez, C., Pham, L. V., Ryberg, L. A., Feng, S., Pedersen, M. S., Mikkelsen, L. S., Belouzard, S., Dubuisson, J., Gottwein, J. M., Fahnøe, U. & Bukh, J., 2020, bioRxiv.

  Research output: Working paper › Preprint › Research

  Open Access

• Elimination af hepatitis C-virus i Danmark

  Translated title of the contribution: Elimination of hepatitis C virus in DenmarkDøssing, A., Røge, B. T., Madsen, L. G., Laursen, A. L., Hansen, J. B., Bukh, J. & Weis, N., 2020, In: Ugeskrift for Laeger. 182, 9

  Research output: Contribution to journal › Journal article › peer-review

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• Equine pegiviruses cause persistent infection of bone marrow and are not associated with hepatitis

  Tomlinson, J. E., Wolfisberg, R., Fahnøe, U., Sharma, H., Renshaw, R. W., Nielsen, L., Nishiuchi, E., Holm, C., Dubovi, E., Rosenberg, B. R., Tennant, B. C., Bukh, J., Kapoor, A., Divers, T. J., Rice, C. M., Van de Walle, G. R. & Scheel, T. K. H., 2020, In: P L o S Pathogens. 16, 7, 23 p., e1008677.

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  20 Citations (Scopus)

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• Evolutionary selection of pestivirus variants with altered or no microRNA dependency

  Kokkonos, K. G., Fossat, N., Nielsen, L., Holm, C., Hepkema, W. M., Bukh, J. & Scheel, T. K. H., 4 Jun 2020, In: Nucleic Acids Research. 48, 10, p. 5555-5571 17 p.

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  9 Citations (Scopus)

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• Global and local envelope protein dynamics of hepatitis C virus determine broad antibody sensitivity

  Augestad, E. H., Castelli, M., Clementi, N., Ströh, L. J., Krey, T., Burioni, R., Mancini, N., Bukh, J. & Prentoe, J., 2020, In: Science Advances. 6, 35, 13 p., eabb5938.

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  29 Citations (Scopus)

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• Identification of specific amino acid residues in the border disease virus glycoprotein E2 that modify virus growth in pig cells but not in sheep cells

  Fahnøe, U., Deng, Y., Davids, N. A., Lohse, L., Bukh, J., Belsham, G. J. & Rasmussen, T. B., 2020, In: The Journal of general virology. 101, 11, p. 1170-1181

  Research output: Contribution to journal › Journal article › Research › peer-review
  2 Citations (Scopus)

• Insights into the unique characteristics of hepatitis C virus genotype 3 revealed by development of a robust sub-genomic DBN3a replicon

  Ward, J. C., Bowyer, S., Chen, S., Fernandes Campos, G. R., Ramirez, S., Bukh, J. & Harris, M., 2020, In: The Journal of general virology. 101, 11, p. 1182-1190 9 p.

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  6 Citations (Scopus)

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• Mutations Identified in the Hepatitis C Virus (HCV) Polymerase of Patients with Chronic HCV Treated with Ribavirin Cause Resistance and Affect Viral Replication Fidelity

  Mejer, N., Fahnøe, U., Galli, A., Ramirez, S., Weiland, O., Benfield, T. & Bukh, J., 2020, In: Antimicrobial Agents and Chemotherapy. 64, 12

  Research output: Contribution to journal › Journal article › Research › peer-review

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  8 Citations (Scopus)

• Reflections on the History of HCV: A Posthumous Examination

  Alter, H. J., Farci, P., Bukh, J. & Purcell, R. H., 2020, In: Clinical Liver Disease. 15, S1, p. S64-S71

  Research output: Contribution to journal › Review › Research › peer-review

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  4 Citations (Scopus)

• Ribavirin inhibition of cell-culture infectious hepatitis C genotype 1-3 viruses is strain-dependent

  Mejer, N., Galli, A., Ramirez, S., Fahnøe, U., Benfield, T. & Bukh, J., 2020, In: Virology. 540, p. 132-140 9 p.

  Research output: Contribution to journal › Journal article › Research › peer-review
  10 Citations (Scopus)

• Specific antibodies induced by immunization with hepatitis B virus-like particles carrying hepatitis C virus envelope glycoprotein 2 epitopes show differential neutralization efficiency

  Czarnota, A., Offersgaard, A., Pihl, A. F., Prentoe, J., Bukh, J., Gottwein, J. M., Bieńkowska-Szewczyk, K. & Grzyb, K., 2020, In: Vaccines. 8, 2, 19 p., 294.

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  17 Citations (Scopus)

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• 2019

  Broadly neutralizing antibodies from an individual that naturally cleared multiple hepatitis c virus infections uncover molecular determinants for E2 targeting and vaccine design

  Keck, Z. Y., Pierce, B. G., Lau, P., Lu, J., Wang, Y., Underwood, A., Bull, R. A., Prentoe, J., Velázquez-Moctezuma, R., Walker, M. R., Luciani, F., Guest, J. D., Fauvelle, C., Baumert, T. F., Bukh, J., Lloyd, A. R. & Foung, S. K. H., 2019, In: P L o S Pathogens. 15, 5, 28 p., e1007772.

  Research output: Contribution to journal › Journal article › Research › peer-review

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  47 Citations (Scopus)

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• Evolutionary Pathways to Persistence of Highly Fit and Resistant Hepatitis C Virus Protease Inhibitor Escape Variants

  Jensen, S. B., Fahnøe, U., Pham, L. V., Serre, S. B. N., Tang, Q., Ghanem, L., Pedersen, M. S., Ramirez, S., Humes, D., Pihl, A. F., Filskov, J., Sølund, C. S., Dietz, J., Fourati, S., Pawlotsky, J. M., Sarrazin, C., Weis, N., Schønning, K., Krarup, H., Bukh, J. & 1 others, Gottwein, J. M., 2019, In: Hepatology. 70, 3, p. 771-787 17 p.

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  45 Citations (Scopus)

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• Full-length open reading frame amplification of hepatitis C virus

  Fahnøe, U. & Bukh, J., 2019, Hepatitis C Virus Protocols. Humana Press, p. 85-91 7 p. (Methods in Molecular Biology, Vol. 1911).

  Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
  20 Citations (Scopus)

• Genetic and structural insights into broad neutralization of hepatitis C virus by human V_H1-69 antibodies

  Tzarum, N., Giang, E., Kong, L., He, L., Prentoe, J., Augestad, E., Hua, Y., Castillo, S., Lauer, G. M., Bukh, J., Zhu, J., Wilson, I. A. & Law, M., 2019, In: Science Advances. 5, 1, 12 p., eaav1882.

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  69 Citations (Scopus)

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• Genome sequence of an unknown subtype of hepatitis C virus genotype 6: Another piece for the taxonomic puzzle

  Pedersen, M. S., Mollerup, S., Nielsen, L. G., Jenssen, H., Bukh, J. & Schønning, K., 2019, In: Microbiology resource announcements. 8, 42, e01030-19.

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  3 Citations (Scopus)

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• HCV genotype 1-6 NS3 residue 80 substitutions impact protease inhibitor activity and promote viral escape

  Pham, L. V., Jensen, S. B., Fahnøe, U., Pedersen, M. S., Tang, Q., Ghanem, L., Ramirez, S., Humes, D., Serre, S. B. N., Schønning, K., Bukh, J. & Gottwein, J. M., 2019, In: Journal of Hepatology. 70, 3, p. 388-397

  Research output: Contribution to journal › Journal article › Research › peer-review
  34 Citations (Scopus)

• HCV p7 as a novel vaccine-target inducing multifunctional CD4⁺ and CD8⁺ T-cells targeting liver cells expressing the viral antigen

  Filskov, J., Andersen, P., Agger, E. M. & Bukh, J., 2019, In: Scientific Reports. 9, 1, 13 p., 14085.

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  24 Citations (Scopus)

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• Hepatitis C Virus–Escape Studies for Human Monoclonal Antibody AR4A Reveal Isolate-Specific Resistance and a High Barrier to Resistance

  Velázquez-moctezuma, R., Galli, A., Law, M., Bukh, J. & Prentoe, J., 2019, In: The Journal of Infectious Diseases. 219, 1, p. 68-79

  Research output: Contribution to journal › Journal article › Research › peer-review

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  13 Citations (Scopus)

• Hepatitis C virus escape studies of human antibody AR3a reveal a high barrier to resistance and novel insights on viral antibody evasion mechanisms

  Velázquez-Moctezuma, R., Galli, A., Law, M., Bukh, J. & Prentoe, J., 2019, In: Journal of Virology. 93, 4, 27 p., e0190918.

  Research output: Contribution to journal › Journal article › peer-review

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  23 Citations (Scopus)

• Hypervariable region 1 and N-linked glycans of hepatitis C regulate virion neutralization by modulating envelope conformations

  Prentoe, J., Velázquez-Moctezuma, R., Augestad, E. H., Galli, A., Wang, R., Law, M., Alter, H. & Bukh, J., 2019, In: Proceedings of the National Academy of Sciences of the United States of America. 116, 20, p. 10039-10047 9 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

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  44 Citations (Scopus)

• In vitro neutralization assay using cultured hepatitis C virus

  Prentoe, J. & Bukh, J., 2019, Hepatitis C Virus Protocols. Humana Press, p. 433-439 7 p. (Methods in Molecular Biology, Vol. 1911).

  Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
  7 Citations (Scopus)

• Replicons of a Rodent Hepatitis C Model Virus Permit Selection of Highly Permissive Cells

  Wolfisberg, R., Holmbeck, K., Nielsen, L., Kapoor, A., Rice, C. M., Bukh, J. & Scheel, T. K. H., 2019, In: Journal of Virology. 93, 19, 19 p., e00733-19.

  Research output: Contribution to journal › Journal article › Research › peer-review

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  14 Citations (Scopus)

• Virus adaptation and selection following challenge of animals vaccinated against classical swine fever virus

  Fahnøe, U., Pedersen, A. G., Johnston, C. M., Orton, R. J., Höper, D., Beer, M., Bukh, J., Belsham, G. J. & Rasmussen, T. B., 2019, In: Viruses. 11, 10, 932.

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  6 Citations (Scopus)

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• 2018

  A near full-length open reading frame next generation sequencing assay for genotyping and identification of resistance-associated variants in hepatitis C virus

  Pedersen, M. S., Fahnøe, U., Hansen, T. A., Pedersen, A. G., Jenssen, H., Bukh, J. & Schønning, K., 2018, In: Journal of Clinical Virology. 105, p. 49-56 8 p.

  Research output: Contribution to journal › Journal article › Research › peer-review
  9 Citations (Scopus)