Research output per year
Research output per year
PhD student, MSc Biology
Blegdamsvej 3, 2200 København N.
Research activity per year
Project: Investigating the anatomy and physiology of neurons that suppress sugar consumption using multiple techniques such as CRISPR, AAV, DREADD, cloning, tissue clearing and geneticaly modified animal in combination with behavioural studies.
Education
Ph.D. on neuronal control of metabolism, at Panum Institute, Department of Biomedicine and Novo Nordisk Foundation Center for Basic Metabolic Research, section for Liver Metabolism and Metabolic Imaging, Copenhagen University.
Advisor: Dr. Matthew Paul Gillum, Ph.D., associate Professor at Copenhagen University
Project: Investigating the anatomy and physiology of neurons that suppress sucrose consumption
February 2015 - present
Double degree in association with Wageningen University, Wageningen, the Netherlands and Groupe ISA, Lille, France
February 2013 – January 2015
Master of Biology, specialization Health and Disease
February 2013 – January 2015
Wageningen University, Wageningen, the Netherlands
Thesis: the nicotinic acetylcholine receptors in undifferentiated pheochromocytoma PC12 cells
Supervisors: Prof. Jens. D. Mikkelsen, NRU, Copenhagen and Dr. Katja Teerds, Wageningen University, Wageningen, the Netherlands
Engineer degree of Food Science, Agriculture and Environment, Engineer student
September 2011 – January 2015
Groupe ISA (Grand Ecole), Agricultural Engineering School, Lille, France
Bachelor of Food Science, Agriculture and Environment
September 2009 – August 2011
Groupe ISA (Grand Ecole), Agricultural Engineering School, Lille, France
Working experience
Advanced Techniques in Molecular Neuroscience: July 2015
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
Gained theoretical and practical experience on multiple techniques such as:
- CRISPR technology (by Dr. Le Cong and Dr. Joe LoTurco)
- Single cell transcriptomics (by Dr. Craig Nelson)
- Brain bows and multispectral imaging (by Dr. Dawen Cai)
- Lentivirus production and injection (by Dr. Bryan Luikart)
- Epigenetics and miRNA (by Dr. Anne Schaefer)
- In utero electroporation (by Dr. Brady Maher)
- Single cell electroporation and imaging (by Dr. Kurt Haas)
- Optogenetics and CLARITY (by Dr. Viviana Gradinaru)
- TRAP (by Dr. Joe Dougherty)
Master thesis: February 2014 – January 2015
Neurobiology Research Unit (NRU), Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark
Focused on the nicotinic acetylcholine receptors in undifferentiated pheochromocytoma PC12 cells using western blot, fluorescent immunocytochemistry and cell culture
Research assistant: June 2013
Experimental Zoology group, Department of Animal Sciences, Wageningen University, Wageningen, the Netherlands
Three-dimensional architecture of the Zebrafish trunk muscle at different stage of the embryonic development
Preparation of embryonic Zebrafish and microscopic analysis with Zeiss LSM-510 confocal microscope
Laboratory research assistant: July 2012 – August 2012
Muséum National d’Histoire Naturelle of Paris, France
Molecular systemic and bar-coding of elasmobranch and teleostei fishes from the ‘POKER’ campaign
PCR preparation, data analysis, data base (BOLDsystem, GenBank, FishBase) management and analysis
Skills related to my education
Cloning and design of GMO animal via plasmid injection (CRISPR technology)
Tissue clearing (CLARITY – PACT )
Extraction and measurement of protein, RNA and DNA level
Western blotting (gel electrophoresis, transfer blot and membranes analysis)
Immunofluorescence staining, microscopic slice preparation (cryostat, microtome, confocal and LCM)
Microglia and neuron isolation from rat pup brain
HEK293 cells, PC12 cells, microglia and neuron culture
FELASA categories ABD (previously called category C)
References
Dr. Matthew Paul Gillum,
Ph.D., associate Professor at Copenhagen University
+45 23 64 89 95
Dr. Kitt Mia Falk Petersen, M.D.
Professor of Internal Medicine at Yale University
203-737-1942
Dr. Linda V. Blomster,
PhD, post-doc at NRU
+46 763 110 091
French: mother tongue
English (TOEFL iBTs 93 points – TOEIC 855 points)
Danish (intermediate – currently taking courses at Studieskolen)
Ingineer, Agriculture, food industry and environment, Groupe ISA
1 Sep 2011 → 8 Jan 2015
Award Date: 8 Jan 2015
Biology, M.Sc, Wageningen Universiteit
1 Feb 2013 → 8 Jan 2015
Award Date: 8 Jan 2015
Research output: Contribution to journal › Journal article › Research › peer-review
Research output: Contribution to journal › Journal article › Research › peer-review
Research output: Contribution to journal › Journal article › Research › peer-review
Research output: Contribution to journal › Journal article › Research › peer-review
Research output: Contribution to journal › Review › Research › peer-review
Research output: Contribution to journal › Journal article › Research › peer-review
Research output: Contribution to journal › Journal article › Research › peer-review
Research output: Contribution to journal › Journal article › Research › peer-review