Santseharay Ramirez Almeida

Research output

Research output per year 2013 2018 2020 2021 2022 2023 2023

• 907 Citations
• 38 Journal article
• 1 Conference abstract for conference
• 1 Poster
• 1 Review
• 1 More
  • 1 Preprint

Research output per year

Research output per year

Search results

• 2023

  An inactivated SARS-CoV-2 vaccine induced cross-neutralizing persisting antibodies and protected against challenge in small animals

  Offersgaard, A., Duarte Hernandez, C. R., Feng, S., Marichal-Gallardo, P., Holmbeck, K., Pihl, A. F., Fernandez-Antunez, C., Alzua, G. P., Hartmann, K. T., Pham, L. V., Zhou, Y., Gammeltoft, K. A., Fahnøe, U., Schneider, U. V., Pedersen, G. K., Jensen, H. E., Christensen, J. P., Ramirez, S., Bukh, J. & Gottwein, J. M., 2023, In: iScience. 26, 2, 105949.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  1 Citation (Scopus)

  60 Downloads (Pure)

• Characterization of multi-DAA resistance using a novel hepatitis C virus genotype 3a infectious culture system

  Fernandez-Antunez, C., Wang, K., Fahnøe, U., Mikkelsen, L. S., Gottwein, J. M., Bukh, J. & Ramirez, S., 2023, In: Hepatology. 78, 2, p. 621-636 16 p.

  Research output: Contribution to journal › Journal article › Research › peer-review
  5 Citations (Scopus)

• Durability and breadth of neutralisation following multiple antigen exposures to SARS-CoV-2 infection and/or COVID-19 vaccination

  Underwood, A. P., Sølund, C., Fernandez-Antunez, C., Villadsen, S. L., Mikkelsen, L. S., Fahnøe, U., Bollerup, S., Winckelmann, A. A., Schneider, U. V., Binderup, A., Vizgirda, G., Sørensen, A. L., Vinten, C. N., Dalegaard, M. I., Ramirez, S., Weis, N. & Bukh, J., 2023, In: EBioMedicine. 89, 104475.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  8 Citations (Scopus)

  26 Downloads (Pure)

• First-in-human use of a modular capsid virus-like vaccine platform: An open-label, non-randomised, phase 1 clinical trial of the SARS-CoV-2 vaccine ABNCoV2

  Smit, M. J., Sander, A. F., Ariaans, M. B. P. A., Fougeroux, C., Heinzel, C., Fendel, R., Esen, M., Kremsner, P. G., Ter Heine, R., Wertheim, H. F., Idorn, M., Paludan, S. R., Underwood, A. P., Binderup, A., Ramirez, S., Bukh, J., Soegaard, M., Erdogan, S. M., Gustavsson, T., Clemmensen, S. & 14 others, Theander, T. G., Salanti, A., Hamborg, M., de Jongh, W. A., McCall, M. B. B., Nielsen, M. A., Mordmüller, B. G., COUGH-1 trial study group, Dagil, R., Goksøyr, L., Hulen, T. M., Brygger, C. M. J., Khalifé, P. K. & Vidal-Calvo, E. E., 2023, In: The Lancet Microbe. 4, 3, p. e140-e148

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  16 Citations (Scopus)

  56 Downloads (Pure)

• Hepatitis C virus alters the morphology and function of peroxisomes

  Martin de Fourchambault, E., Callens, N., Saliou, J. M., Fourcot, M., Delos, O., Barois, N., Thorel, Q., Ramirez, S., Bukh, J., Cocquerel, L., Bertrand-Michel, J., Marot, G., Sebti, Y., Dubuisson, J. & Rouillé, Y., 2023, In: Frontiers in Microbiology. 14, 1254728.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  6 Citations (Scopus)

  7 Downloads (Pure)

• Hepatitis C virus RNA is 5'-capped with flavin adenine dinucleotide

  Sherwood, A. V., Rivera-Rangel, L. R., Ryberg, L. A., Larsen, H. S., Anker, K. M., Costa, R., Vågbø, C. B., Jakljevič, E., Pham, L. V., Fernandez-Antunez, C., Indrisiunaite, G., Podolska-Charlery, A., Grothen, J. E. R., Langvad, N. W., Fossat, N., Offersgaard, A., Al-Chaer, A., Nielsen, L., Kuśnierczyk, A., Sølund, C. & 8 others, Weis, N., Gottwein, J. M., Holmbeck, K., Bottaro, S., Ramirez, S., Bukh, J., Scheel, T. K. H. & Vinther, J., 2023, In: Nature. 619, p. 811-818

  Research output: Contribution to journal › Journal article › Research › peer-review

  File
  12 Citations (Scopus)

• Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection

  Fossat, N., Lundsgaard, E. A., Costa, R., Rivera-Rangel, L. R., Nielsen, L., Mikkelsen, L. S., Ramirez, S., Bukh, J. & Scheel, T. K. H., 2023, In: Cell Reports. 42, 4, 112282.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  9 Citations (Scopus)

  82 Downloads (Pure)

• SARS-CoV-2 spike HexaPro formulated in aluminium hydroxide and administered in an accelerated vaccination schedule partially protects Syrian Hamsters against viral challenge despite low neutralizing antibody responses

  Christensen, D., Polacek, C., Sheward, D. J., Hanke, L., McInerney, G., Murrell, B., Hartmann, K. T., Jensen, H. E., Zimmermann, J., Jungersen, G., Illigen, K. E., Isling, L. K., Fernandez-Antunez, C., Ramirez, S., Bukh, J. & Pedersen, G. K., 2023, In: Frontiers in Immunology. 14, 941281.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  3 Citations (Scopus)

  13 Downloads (Pure)

• Substitutions in SARS-CoV-2 Mpro Selected by Protease Inhibitor Boceprevir Confer Resistance to Nirmatrelvir

  Gammeltoft, K. A., Zhou, Y., Ryberg, L. A., Pham, L. V., Binderup, A., Duarte Hernandez, C. R., Offersgaard, A., Fahnøe, U., Peters, G. H. J., Ramirez, S., Bukh, J. & Gottwein, J. M., 2023, In: Viruses. 15, 9, 17 p., 1970.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  7 Citations (Scopus)

  12 Downloads (Pure)
• 2022

  Analysis of Neutralization Titers against SARS-CoV-2 in Health-Care Workers Vaccinated with Prime-Boost mRNA–mRNA or Vector–mRNA COVID-19 Vaccines

  Sølund, C., Underwood, A. P., Fernandez-Antunez, C., Bollerup, S., Mikkelsen, L. S., Villadsen, S. L., Fahnøe, U., Winckelmann, A. A., Feng, S., Nørløv Vinten, C. A., Dalegaard, M. I., Vizgirda, G., Sørensen, A. L., Ramirez, S., Bukh, J. & Weis, N., 2022, In: Vaccines. 10, 1, 75.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  10 Citations (Scopus)

  69 Downloads (Pure)

• Establishment of high-titer HCV and SARS-CoV-2 production in a scalable packed-bed bioreactor for inactivated vaccines inducing neutralizing antibodies in animals

  Offersgaard, A. F., Hernandez, C. R. D., Finne Pihl, A., Feng, S., Marichal-Gallardo, P., Venkatesan, N. P., Lin, X., Fernandez-Antunez, C., Pena Alzua, G., Costa, R., Hartmann, K. T., Pham, L., Zhou, Y., Gammeltoft, K. A., Fahnøe, U., Scheel, T. K. H., Schneider, U. V., Jensen, H. E., Christensen, J. P., Ramirez Almeida, S. & 4 others, Reichl, U., Genzel, Y., Bukh, J. & Gottwein, J. M., 6 Jul 2022.

  Research output: Contribution to conference › Poster › Communication

• Establishment of high-titer HCV and SARS-CoV-2 production in a scalable packed-bed bioreactor for inactivated vaccines inducing neutralizing antibodies in animals

  Offersgaard, A. F., Hernandez, C. R. D., Finne Pihl, A., Feng, S., Marichal-Gallardo, P., Holmbeck, K., Venkatesan, N. P., Lin, X., Fernandez-Antunez, C., Pena Alzua, G., Costa, R., Hartmann, K. T., Pham, L., Zhou, Y., Gammeltoft, K. A., Fahnøe, U., Scheel, T. K. H., Schneider, U. V., Jensen, H. E., Christensen, J. P. & 5 others, Ramirez Almeida, S., Reichl, U., Genzel, Y., Bukh, J. & Gottwein, J. M., 6 Jul 2022.

  Research output: Contribution to conference › Conference abstract for conference › Communication

• HCV genome-wide analysis for development of efficient culture systems and unravelling of antiviral resistance in genotype 4

  Pham, L. V., Pedersen, M. S., Fahnøe, U., Fernandez-Antunez, C., Humes, D., Schønning, K., Ramirez, S. & Bukh, J., 2022, In: Gut. 71, p. 627-642 20323585.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  8 Citations (Scopus)

  36 Downloads (Pure)

• Nirmatrelvir-resistant SARS-CoV-2 variants with high fitness in an infectious cell culture system

  Zhou, Y., Gammeltoft, K. A., Ryberg, L. A., Pham, L. V., Tjørnelund, H. D., Binderup, A., Hernandez, C. R. D., Fernandez-Antunez, C., Offersgaard, A., Fahnøe, U., Peters, G. H. J., Ramirez, S., Bukh, J. & Gottwein, J. M., 2022, In: Science Advances. 8, 51, eadd7197.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  81 Citations (Scopus)

  10 Downloads (Pure)

• Protection against SARS-CoV-2 transmission by a parenteral prime—Intranasal boost vaccine strategy

  Christensen, D., Polacek, C., Sheward, D. J., Hanke, L., Moliner-morro, A., Mcinerney, G., Murrell, B., Hartmann, K. T., Jensen, H. E., Jungersen, G., Illigen, K., Isling, L. K., Jensen, R. F., Hansen, J. S., Rosenkrands, I., Fernandez-antunez, C., Ramirez, S., Follmann, F., Bukh, J. & Pedersen, G. K., 2022, In: EBioMedicine. 84, 104248.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  18 Citations (Scopus)

  15 Downloads (Pure)

• Versatile SARS-CoV-2 Reverse-Genetics Systems for the Study of Antiviral Resistance and Replication

  Fahnøe, U., Pham, L. V., Fernandez-Antunez, C., Costa, R., Rivera-Rangel, L. R., Galli, A., Feng, S., Mikkelsen, L. S., Gottwein, J. M., Scheel, T. K. H., Ramirez, S. & Bukh, J., 2022, In: Viruses. 14, 2, 172.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  13 Citations (Scopus)

  55 Downloads (Pure)
• 2021

  Efficacy of ion-channel inhibitors amantadine, memantine and rimantadine for the treatment of SARS-CoV-2 in vitro

  Zhou, Y., Gammeltoft, K. A., Galli, A., Offersgaard, A., Fahnøe, U., Ramirez, S., Bukh, J. & Gottwein, J. M., 2021, In: Viruses. 13, 10, 2082.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  23 Citations (Scopus)

  50 Downloads (Pure)

• Hepatitis C Virus Protease Inhibitors Show Differential Efficacy and Interactions with Remdesivir for Treatment of SARS-CoV-2 in Vitro

  Gammeltoft, K. A., Zhou, Y., Duarte Hernandez, C. R., Galli, A., Offersgaard, A., Costa, R., Pham, L. V., Fahnøe, U., Feng, S., Scheel, T. K. H., Ramirez, S., Bukh, J. & Gottwein, J. M., 2021, In: Antimicrobial Agents and Chemotherapy. 65, 9, e02680-20.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  27 Citations (Scopus)

  32 Downloads (Pure)

• In vitro efficacy of artemisinin-based treatments against SARS-CoV-2

  Zhou, Y., Gilmore, K., Ramirez, S., Settels, E., Gammeltoft, K. A., Pham, L. V., Fahnøe, U., Feng, S., Offersgaard, A., Trimpert, J., Bukh, J., Osterrieder, K., Gottwein, J. M. & Seeberger, P. H., 2021, In: Scientific Reports. 11, 14571.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  64 Citations (Scopus)

  61 Downloads (Pure)

• Lipid Droplets Accumulation during Hepatitis C Virus Infection in Cell-Culture Varies among Genotype 1-3 Strains and Does Not Correlate with Virus Replication

  Galli, A., Ramirez, S. & Bukh, J., 2021, In: Viruses. 13, 3, 389.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  8 Citations (Scopus)

  26 Downloads (Pure)

• Neutralisation titres against SARS-CoV-2 are sustained 6 months after onset of symptoms in individuals with mild COVID-19

  Underwood, A. P., Sølund, C., Fernandez-Antunez, C., Villadsen, S. L., Winckelmann, A. A., Bollerup, S., Mikkelsen, L. S., Sørensen, A. L., Feng, S., Fahnøe, U., Lassauniere, R., Fomsgaard, A., Ramirez, S., Weis, N. & Bukh, J., 2021, In: EBioMedicine. 71, 103519.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  14 Citations (Scopus)

  36 Downloads (Pure)

• Overcoming culture restriction for SARS-CoV-2 in human cells facilitates the screening of compounds inhibiting viral replication

  Ramirez, S., Fernandez-Antunez, C., Galli, A., Underwood, A., Pham, L. V., Ryberg, L. A., Feng, S., Pedersen, M. S., Mikkelsen, L. S., Belouzard, S., Dubuisson, J., Sølund, C., Weis, N., Gottwein, J. M., Fahnøe, U. & Bukh, J., 2021, In: Antimicrobial Agents and Chemotherapy. 65, 7, 20 p., e00097-21.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access
  55 Citations (Scopus)

• Sars-cov-2 production in a scalable high cell density bioreactor

  Offersgaard, A., Hernandez, C. R. D., Pihl, A. F., Costa, R., Venkatesan, N. P., Lin, X., Van Pham, L., Feng, S., Fahnøe, U., Scheel, T. K. H., Ramirez, S., Reichl, U., Bukh, J., Genzel, Y. & Gottwein, J. M., 2021, In: Vaccines. 9, 7, 706.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  19 Citations (Scopus)

  65 Downloads (Pure)

• Viral genome wide association study identifies novel hepatitis C virus polymorphisms associated with sofosbuvir treatment failure

  STOP-HCV Consortium, 2021, In: Nature Communications. 12, 1, 11 p., 6105.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  12 Citations (Scopus)

  15 Downloads (Pure)
• 2020

  Cell culture studies of the efficacy and barrier to resistance of sofosbuvir-velpatasvir and glecaprevir-pibrentasvir against hepatitis C virus genotypes 2a, 2b, and 2C

  Ramirez, S., Fernandez-Antunez, C., Mikkelsen, L. S., Pedersen, J., Li, Y. P. & Bukh, J., 2020, In: Antimicrobial Agents and Chemotherapy. 64, 3, e01888.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access
  14 Citations (Scopus)

• Efficient culture of SARS-CoV-2 in human hepatoma cells enhances viability of the virus in human lung cancer cell lines permitting the screening of antiviral compounds

  Ramirez, S., Fernandez-Antunez, C., Pham, L. V., Ryberg, L. A., Feng, S., Pedersen, M. S., Mikkelsen, L. S., Belouzard, S., Dubuisson, J., Gottwein, J. M., Fahnøe, U. & Bukh, J., 2020, bioRxiv.

  Research output: Working paper › Preprint

  Open Access

• Insights into the unique characteristics of hepatitis C virus genotype 3 revealed by development of a robust sub-genomic DBN3a replicon

  Ward, J. C., Bowyer, S., Chen, S., Fernandes Campos, G. R., Ramirez, S., Bukh, J. & Harris, M., 2020, In: The Journal of general virology. 101, 11, p. 1182-1190 9 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  6 Citations (Scopus)

  51 Downloads (Pure)

• Mutations Identified in the Hepatitis C Virus (HCV) Polymerase of Patients with Chronic HCV Treated with Ribavirin Cause Resistance and Affect Viral Replication Fidelity

  Mejer, N., Fahnøe, U., Galli, A., Ramirez, S., Weiland, O., Benfield, T. & Bukh, J., 2020, In: Antimicrobial Agents and Chemotherapy. 64, 12

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access
  8 Citations (Scopus)

• Ribavirin inhibition of cell-culture infectious hepatitis C genotype 1-3 viruses is strain-dependent

  Mejer, N., Galli, A., Ramirez, S., Fahnøe, U., Benfield, T. & Bukh, J., 2020, In: Virology. 540, p. 132-140 9 p.

  Research output: Contribution to journal › Journal article › Research › peer-review
  10 Citations (Scopus)
• 2019

  Evolutionary Pathways to Persistence of Highly Fit and Resistant Hepatitis C Virus Protease Inhibitor Escape Variants

  Jensen, S. B., Fahnøe, U., Pham, L. V., Serre, S. B. N., Tang, Q., Ghanem, L., Pedersen, M. S., Ramirez, S., Humes, D., Pihl, A. F., Filskov, J., Sølund, C. S., Dietz, J., Fourati, S., Pawlotsky, J. M., Sarrazin, C., Weis, N., Schønning, K., Krarup, H., Bukh, J. & 1 others, Gottwein, J. M., 2019, In: Hepatology. 70, 3, p. 771-787 17 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access

  File
  43 Citations (Scopus)

  200 Downloads (Pure)

• HCV genotype 1-6 NS3 residue 80 substitutions impact protease inhibitor activity and promote viral escape

  Pham, L. V., Jensen, S. B., Fahnøe, U., Pedersen, M. S., Tang, Q., Ghanem, L., Ramirez, S., Humes, D., Serre, S. B. N., Schønning, K., Bukh, J. & Gottwein, J. M., 2019, In: Journal of Hepatology. 70, 3, p. 388-397

  Research output: Contribution to journal › Journal article › Research › peer-review
  34 Citations (Scopus)
• 2018

  Current status and future development of infectious cell-culture models for the major genotypes of hepatitis C virus: Essential tools in testing of antivirals and emerging vaccine strategies

  Ramirez, S. & Bukh, J., 2018, In: Antiviral Research. 158, p. 264-287 24 p.

  Research output: Contribution to journal › Review › Research › peer-review
  40 Citations (Scopus)

• Efficacy of NS5A Inhibitors Against Hepatitis C Virus Genotypes 1–7 and Escape Variants

  Gottwein, J. M., Pham, L. V., Mikkelsen, L. S., Ghanem, L., Ramirez, S., Scheel, T. K. H., Carlsen, T. H. R. & Bukh, J., Apr 2018, In: Gastroenterology. 154, 5, p. 1435-1448 14 p.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access
  85 Citations (Scopus)

• HCV Genotype 6a Escape from and Resistance to Velpatasvir, Pibrentasvir, and Sofosbuvir in Robust Infectious Cell Culture Models

  Pham, L., Ramirez Almeida, S., Gottwein, J. M., Fahnøe, U., Li, Y., Pedersen, J. & Bukh, J., 2018, In: Gastroenterology. 154, 8, p. 2194-2208.e12

  Research output: Contribution to journal › Journal article › Research › peer-review
  42 Citations (Scopus)

• Recombinant hepatitis C virus genotype 5a infectious cell culture systems expressing minimal JFH1 NS5B sequences permit polymerase inhibitor studies

  Humes, D., Ramirez, S., Jensen, T. B., Li, Y. P., Gottwein, J. M. & Bukh, J., 2018, In: Virology. 522, p. 177-192 16 p.

  Research output: Contribution to journal › Journal article › Research › peer-review
  4 Citations (Scopus)

• Ribavirin-induced mutagenesis across the complete open reading frame of hepatitis C virus genotypes 1a and 3a

  Mejer, N., Fahnøe, U., Galli, A., Ramirez, S., Benfield, T. & Bukh, J., 2018, In: Journal of General Virology. 99, 8, p. 1066-1077 12 p., 001095.

  Research output: Contribution to journal › Journal article › Research › peer-review
  12 Citations (Scopus)
• 2017

  Efficient hepatitis c virus genotype 1b core-NS5A recombinants permit efficacy testing of protease and NS5A inhibitors

  Pham, L. V., Ramirez Almeida, S., Carlsen, T. H. R., Li, Y-P., Gottwein, J. M. & Bukh, J., 2017, In: Antimicrobial Agents and Chemotherapy. 61, 6, 19 p., e00037-17.

  Research output: Contribution to journal › Journal article › Research › peer-review

  Open Access
  10 Citations (Scopus)
• 2016

  Robust HCV Genotype 3a Infectious Cell Culture System Permits Identification of Escape Variants With Resistance to Sofosbuvir

  Ramirez Almeida, S., Mikkelsen, L. S., Gottwein, J. M. & Bukh, J., Nov 2016, In: Gastroenterology. 151, 5, p. 973-985.e2

  Research output: Contribution to journal › Journal article › Research › peer-review
  74 Citations (Scopus)
• 2015

  Efficient infectious cell culture systems of the hepatitis C virus (HCV) prototype strains HCV-1 and H77

  Li, Y-P., Ramirez, S., Mikkelsen, L. & Bukh, J., Jan 2015, In: Journal of Virology. 89, 1, p. 811-23 13 p.

  Research output: Contribution to journal › Journal article › Research › peer-review
  37 Citations (Scopus)

• Substitutions at NS3 Residue 155, 156, or 168 of Hepatitis C Virus Genotypes 2 to 6 Induce Complex Patterns of Protease Inhibitor Resistance

  Jensen, S. B., Serre, S. B. N., Humes, D. G., Ramirez Almeida, S., Li, Y-P., Bukh, J. & Gottwein, J. M., Dec 2015, In: Antimicrobial Agents and Chemotherapy. 59, 12, p. 7426-7436 11 p.

  Research output: Contribution to journal › Journal article › Research › peer-review
  35 Citations (Scopus)
• 2014

  Nationwide experience of treatment with protease inhibitors in chronic hepatitis C patients in denmark: identification of viral resistance mutations

  Sølund, C., Krarup, H., Ramirez, S., Thielsen, P., Røge, B. T., Lunding, S., Barfod, T. S., Madsen, L. G., Tarp, B., Christensen, P. B., Gerstoft, J., Laursen, A. L., Bukh, J., Weis, N. & DANHEP Group, 2014, In: PLOS ONE. 9, 12, p. 1-20 20 p., e113034.

  Research output: Contribution to journal › Journal article › Research › peer-review
  12 Citations (Scopus)
• 2013

  Characterization of hepatitis C virus recombinants with chimeric E1/E2 envelope proteins and identification of single amino acids in the E2 stem region important for entry

  Carlsen, T. H. R., Scheel, T. K. H., Ramirez, S., Foung, S. K. H. & Bukh, J., 2013, In: Journal of Virology. 87, 3, p. 1385-99 15 p.

  Research output: Contribution to journal › Journal article › Research › peer-review
  22 Citations (Scopus)