Personal profile
Short presentation
My research is focused on diagnostic/therapeutic applications of monoclonal antibodies (mAbs). We develop mAbs (https://glycomics.ku.dk/research--resources/) to probe biosynthesis of mucin-type O-glycosylation and truncated cancer O-glycans. Our mAbs directed to mucins with truncated O-glycans have cancer-specific reactivity. One mAb designated 5E5 is directed to a cancer-specific epitope on mucin-1, and this is now in a phase 1 clinical trial (CAR-T) at UPENN/Tmunity with collaborators. A novel cell-based mucin display platform is used to explore immunogenicity of human cancer mucins for vaccine developments and to generate novel cancer-specific mAbs. One of these novel mAbs is currently under preclinical evaluation by a company.
Keywords
- Faculty of Health and Medical Sciences
Collaborations and top research areas from the last five years
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Structure of the Fab fragment of a humanized 5E5 antibody to a cancer-specific Tn-MUC1 epitope
Li, W., Mandel, U., van Faassen, H., Parker, M. J., Legg, M. S. G., Hussack, G., Clausen, H. & Evans, S. V., 2025, In: Acta crystallographica. Section D, Structural biology. 81, p. 223-233Research output: Contribution to journal › Journal article › Research › peer-review
Open AccessFile22 Downloads (Pure) -
A universal GlycoDesign for lysosomal replacement enzymes to improve circulation time and biodistribution
Chen, Y. H., Tian, W., Yasuda, M., Ye, Z., Song, M., Mandel, U., Kristensen, C., Povolo, L., Marques, A. R. A., Čaval, T., Heck, A. J. R., Sampaio, J. L., Johannes, L., Tsukimura, T., Desnick, R., Vakhrushev, S. Y., Yang, Z. & Clausen, H., 2023, In: Frontiers in Bioengineering and Biotechnology. 11, 1128371.Research output: Contribution to journal › Journal article › Research › peer-review
Open AccessFile6 Citations (Scopus)93 Downloads (Pure) -
Display of the human mucinome with defined O-glycans by gene engineered cells
Nason, R., Büll, C., Konstantinidi, A., Sun, L., Ye, Z., Halim, A., Du, W., Sørensen, D. M., Durbesson, F., Furukawa, S., Mandel, U., Joshi, H. J., Dworkin, L. A., Hansen, L., David, L., Iverson, T. M., Bensing, B. A., Sullam, P. M., Varki, A. & Vries, E. D. & 6 others, , 2021, In: Nature Communications. 12, 1, 16 p., 4070.Research output: Contribution to journal › Journal article › Research › peer-review
Open AccessFile102 Citations (Scopus)166 Downloads (Pure) -
Golgi maturation-dependent glycoenzyme recycling controls glycosphingolipid biosynthesis and cell growth via GOLPH3
Rizzo, R., Russo, D., Kurokawa, K., Sahu, P., Lombardi, B., Supino, D., Zhukovsky, M. A., Vocat, A., Pothukuchi, P., Kunnathully, V., Capolupo, L., Boncompain, G., Vitagliano, C., Zito Marino, F., Aquino, G., Montariello, D., Henklein, P., Mandrich, L., Botti, G. & Clausen, H. & 11 others, , 2021, In: EMBO Journal. 40, e107238.Research output: Contribution to journal › Journal article › Research › peer-review
Open Access87 Citations (Scopus) -
Isoforms of MUC16 activate oncogenic signaling through EGF receptors to enhance the progression of pancreatic cancer
Thomas, D., Sagar, S., Liu, X., Lee, H. R., Grunkemeyer, J. A., Grandgenett, P. M., Caffrey, T., O'Connell, K. A., Swanson, B., Marcos-Silva, L., Steentoft, C., Wandall, H. H., Maurer, H. C., Peng, X. L., Yeh, J. J., Qiu, F., Yu, F., Madiyalakan, R., Olive, K. P. & Mandel, U. & 3 others, , 2021, In: Molecular Therapy. 29, 4, p. 1557-1571Research output: Contribution to journal › Journal article › Research › peer-review
Open Access56 Citations (Scopus) -
A Genetic Approach to Display and Dissect the Cancer-Associated O-Glycoepitome
Clausen, H., Nason, R., Mandel, U. & Narimatsu, Y., 2020, In: FASEB Journal. 34, S1, 1 p.Research output: Contribution to journal › Conference abstract in journal › Research › peer-review
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Cancer-associated hypersialylated MUC1 drives the differentiation of human monocytes into macrophages with a pathogenic phenotype
Beatson, R., Graham, R., Grundland Freile, F., Cozzetto, D., Kannambath, S., Pfeifer, E., Woodman, N., Owen, J., Nuamah, R., Mandel, U., Pinder, S., Gillett, C., Noll, T., Bouybayoune, I., Taylor-Papadimitriou, J. & Burchell, J. M., 2020, In: Communications Biology . 3, 15 p., 644.Research output: Contribution to journal › Journal article › Research › peer-review
Open AccessFile59 Citations (Scopus)118 Downloads (Pure) -
An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells
Narimatsu, Y., Joshi, H. J., Nason, R., Van Coillie, J., Karlsson, R., Sun, L., Ye, Z., Chen, Y.-H., Schjoldager, K. T., Steentoft, C., Furukawa, S., Bensing, B. A., Sullam, P. M., Thompson, A. J., Paulson, J. C., Büll, C., Adema, G. J., Mandel, U., Hansen, L. & Bennett, E. P. & 4 others, , 2019, In: Molecular Cell. 75, 2, p. 394-407, e1-e5Research output: Contribution to journal › Journal article › Research › peer-review
Open Access217 Citations (Scopus)