5S rRNA genes in Pisum: Sequence, long range and chromosomal organization

T. H.N. Ellis*, D. Lee, C. M. Thomas, P. R. Simpson, W. G. Cleary, M. A. Newman, K. W.G. Burcham

*Corresponding author for this work

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Abstract

We have employed a combination of techniques to examine the organization of pea 5S rRNA genes. These include the analysis of length variant interspersion patterns in cosmid clones, sequence analysis, Southern analysis of both conventional gels and field inversion gels and in situ hybridization. From these analyses we conclude that the 5S rRNA genes of pea are arranged in three major tandem arrays which are represented by three large EcoRI fragments and that these correspond to the three sites of in situ hybridization in the haploid pea complement

Original languageEnglish
JournalMGG Molecular & General Genetics
Volume214
Issue number2
Pages (from-to)333-342
Number of pages10
ISSN0026-8925
DOIs
Publication statusPublished - Oct 1988

Keywords

  • 5S rRNA genes
  • Field inversion gel electrophresis
  • In situ hybridization
  • Long range mapping
  • Pea (Pisum)

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