5S rRNA genes in Pisum: Sequence, long range and chromosomal organization

T. H.N. Ellis; D. Lee; C. M. Thomas; P. R. Simpson; W. G. Cleary; M. A. Newman; K. W.G. Burcham

doi:10.1007/BF00337732

5S rRNA genes in Pisum: Sequence, long range and chromosomal organization

T. H.N. Ellis^*, D. Lee, C. M. Thomas, P. R. Simpson, W. G. Cleary, M. A. Newman, K. W.G. Burcham

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

We have employed a combination of techniques to examine the organization of pea 5S rRNA genes. These include the analysis of length variant interspersion patterns in cosmid clones, sequence analysis, Southern analysis of both conventional gels and field inversion gels and in situ hybridization. From these analyses we conclude that the 5S rRNA genes of pea are arranged in three major tandem arrays which are represented by three large EcoRI fragments and that these correspond to the three sites of in situ hybridization in the haploid pea complement

Original language	English
Journal	MGG Molecular & General Genetics
Volume	214
Issue number	2
Pages (from-to)	333-342
Number of pages	10
ISSN	0026-8925
DOIs	https://doi.org/10.1007/BF00337732
Publication status	Published - Oct 1988

Keywords

5S rRNA genes
Field inversion gel electrophresis
In situ hybridization
Long range mapping
Pea (Pisum)

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10.1007/BF00337732

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title = "5S rRNA genes in Pisum: Sequence, long range and chromosomal organization",

abstract = "We have employed a combination of techniques to examine the organization of pea 5S rRNA genes. These include the analysis of length variant interspersion patterns in cosmid clones, sequence analysis, Southern analysis of both conventional gels and field inversion gels and in situ hybridization. From these analyses we conclude that the 5S rRNA genes of pea are arranged in three major tandem arrays which are represented by three large EcoRI fragments and that these correspond to the three sites of in situ hybridization in the haploid pea complement",

keywords = "5S rRNA genes, Field inversion gel electrophresis, In situ hybridization, Long range mapping, Pea (Pisum)",

author = "Ellis, {T. H.N.} and D. Lee and Thomas, {C. M.} and Simpson, {P. R.} and Cleary, {W. G.} and Newman, {M. A.} and Burcham, {K. W.G.}",

year = "1988",

month = oct,

doi = "10.1007/BF00337732",

language = "English",

volume = "214",

pages = "333--342",

journal = "Molecular General Genetics",

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T1 - 5S rRNA genes in Pisum

T2 - Sequence, long range and chromosomal organization

AU - Ellis, T. H.N.

AU - Lee, D.

AU - Thomas, C. M.

AU - Simpson, P. R.

AU - Cleary, W. G.

AU - Newman, M. A.

AU - Burcham, K. W.G.

PY - 1988/10

Y1 - 1988/10

N2 - We have employed a combination of techniques to examine the organization of pea 5S rRNA genes. These include the analysis of length variant interspersion patterns in cosmid clones, sequence analysis, Southern analysis of both conventional gels and field inversion gels and in situ hybridization. From these analyses we conclude that the 5S rRNA genes of pea are arranged in three major tandem arrays which are represented by three large EcoRI fragments and that these correspond to the three sites of in situ hybridization in the haploid pea complement

AB - We have employed a combination of techniques to examine the organization of pea 5S rRNA genes. These include the analysis of length variant interspersion patterns in cosmid clones, sequence analysis, Southern analysis of both conventional gels and field inversion gels and in situ hybridization. From these analyses we conclude that the 5S rRNA genes of pea are arranged in three major tandem arrays which are represented by three large EcoRI fragments and that these correspond to the three sites of in situ hybridization in the haploid pea complement

KW - 5S rRNA genes

KW - Field inversion gel electrophresis

KW - In situ hybridization

KW - Long range mapping

KW - Pea (Pisum)

UR - http://www.scopus.com/inward/record.url?scp=0024099677&partnerID=8YFLogxK

U2 - 10.1007/BF00337732

DO - 10.1007/BF00337732

M3 - Journal article

C2 - 3237209

AN - SCOPUS:0024099677

VL - 214

SP - 333

EP - 342

JO - Molecular General Genetics

JF - Molecular General Genetics

SN - 0026-8925

IS - 2

ER -

5S rRNA genes in Pisum: Sequence, long range and chromosomal organization

Abstract

Keywords

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