A distinct dexamethasone-dependent gene expression profile in the lungs of COVID-19 patients

Ulrik Fahnøe; Andreas Ronit; Ronan M G Berg; Sofie E Jørgensen; Trine H Mogensen; Alexander P Underwood; Troels K H Scheel; Jens Bukh; Ronni R Plovsing

doi:10.1093/infdis/jiac218

A distinct dexamethasone-dependent gene expression profile in the lungs of COVID-19 patients

Ulrik Fahnøe, Andreas Ronit, Ronan M G Berg, Sofie E Jørgensen, Trine H Mogensen, Alexander P Underwood, Troels K H Scheel, Jens Bukh, Ronni R Plovsing

Research output: Contribution to journal › Journal article › Research › peer-review

7 Citations (Scopus)

Abstract

The effects of dexamethasone (DXM) treatment on pulmonary immunity in COVID-19 associated acute respiratory distress syndrome (CARDS) remain insufficiently understood. We performed transcriptomic RNA-seq analysis of bronchoalveolar lavage fluid from 20 mechanically ventilated patients: 12 with CARDS (DXM+ or DXM-) and 8 non-COVID-19 critically ill controls. CARDS (+DXM) was characterized by upregulation of genes related to B-cell and complement pathway activation, antigen presentation, phagocytosis and FC-gamma receptor signalling. Most ISGs were upregulated in CARDS, particularly in CARDS (-DXM). In conclusion, DXM treatment was not associated with regulation of pro-inflammatory pathways in CARDS but with regulation of other local immune responses.

Original language	English
Journal	The Journal of Infectious Diseases
Volume	226
Issue number	12
Pages (from-to)	2137–2141
ISSN	0022-1899
DOIs	https://doi.org/10.1093/infdis/jiac218
Publication status	Published - 2022

Bibliographical note

© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America and HIV Medicine Association. All rights reserved. For permissions, please e-mail: [email protected].

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10.1093/infdis/jiac218

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213855/pdf/jiac218.pdfLicence: Other

Cite this

@article{81ba7e3a613d41a08f0d19e1b0c18e30,

title = "A distinct dexamethasone-dependent gene expression profile in the lungs of COVID-19 patients",

abstract = "The effects of dexamethasone (DXM) treatment on pulmonary immunity in COVID-19 associated acute respiratory distress syndrome (CARDS) remain insufficiently understood. We performed transcriptomic RNA-seq analysis of bronchoalveolar lavage fluid from 20 mechanically ventilated patients: 12 with CARDS (DXM+ or DXM-) and 8 non-COVID-19 critically ill controls. CARDS (+DXM) was characterized by upregulation of genes related to B-cell and complement pathway activation, antigen presentation, phagocytosis and FC-gamma receptor signalling. Most ISGs were upregulated in CARDS, particularly in CARDS (-DXM). In conclusion, DXM treatment was not associated with regulation of pro-inflammatory pathways in CARDS but with regulation of other local immune responses.",

author = "Ulrik Fahn{\o}e and Andreas Ronit and Berg, {Ronan M G} and J{\o}rgensen, {Sofie E} and Mogensen, {Trine H} and Underwood, {Alexander P} and Scheel, {Troels K H} and Jens Bukh and Plovsing, {Ronni R}",

note = "{\textcopyright} The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America and HIV Medicine Association. All rights reserved. For permissions, please e-mail: [email protected].",

year = "2022",

doi = "10.1093/infdis/jiac218",

language = "English",

volume = "226",

pages = "2137–2141",

journal = "The Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

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TY - JOUR

T1 - A distinct dexamethasone-dependent gene expression profile in the lungs of COVID-19 patients

AU - Fahnøe, Ulrik

AU - Ronit, Andreas

AU - Berg, Ronan M G

AU - Jørgensen, Sofie E

AU - Mogensen, Trine H

AU - Underwood, Alexander P

AU - Scheel, Troels K H

AU - Bukh, Jens

AU - Plovsing, Ronni R

N1 - © The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America and HIV Medicine Association. All rights reserved. For permissions, please e-mail: [email protected].

PY - 2022

Y1 - 2022

N2 - The effects of dexamethasone (DXM) treatment on pulmonary immunity in COVID-19 associated acute respiratory distress syndrome (CARDS) remain insufficiently understood. We performed transcriptomic RNA-seq analysis of bronchoalveolar lavage fluid from 20 mechanically ventilated patients: 12 with CARDS (DXM+ or DXM-) and 8 non-COVID-19 critically ill controls. CARDS (+DXM) was characterized by upregulation of genes related to B-cell and complement pathway activation, antigen presentation, phagocytosis and FC-gamma receptor signalling. Most ISGs were upregulated in CARDS, particularly in CARDS (-DXM). In conclusion, DXM treatment was not associated with regulation of pro-inflammatory pathways in CARDS but with regulation of other local immune responses.

AB - The effects of dexamethasone (DXM) treatment on pulmonary immunity in COVID-19 associated acute respiratory distress syndrome (CARDS) remain insufficiently understood. We performed transcriptomic RNA-seq analysis of bronchoalveolar lavage fluid from 20 mechanically ventilated patients: 12 with CARDS (DXM+ or DXM-) and 8 non-COVID-19 critically ill controls. CARDS (+DXM) was characterized by upregulation of genes related to B-cell and complement pathway activation, antigen presentation, phagocytosis and FC-gamma receptor signalling. Most ISGs were upregulated in CARDS, particularly in CARDS (-DXM). In conclusion, DXM treatment was not associated with regulation of pro-inflammatory pathways in CARDS but with regulation of other local immune responses.

U2 - 10.1093/infdis/jiac218

DO - 10.1093/infdis/jiac218

M3 - Journal article

C2 - 35639922

SN - 0022-1899

VL - 226

SP - 2137

EP - 2141

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

IS - 12

ER -