TY - JOUR
T1 - A human gut microbial gene catalogue established by metagenomic sequencing
AU - Qin, Junjie
AU - Li, Ruiqiang
AU - Raes, Jeroen
AU - Arumugam, Manimozhiyan
AU - Burgdorf, Kristoffer Solvsten
AU - Manichanh, Chaysavanh
AU - Nielsen, Trine
AU - Pons, Nicolas
AU - Levenez, Florence
AU - Yamada, Takuji
AU - Mende, Daniel R.
AU - Li, Junhua
AU - Xu, Junming
AU - Li, Shaochuan
AU - Li, Dongfang
AU - Cao, Jianjun
AU - Wang, Bo
AU - Liang, Huiqing
AU - Zheng, Huisong
AU - Xie, Yinlong
AU - Tap, Julien
AU - Lepage, Patricia
AU - dos Santos, Marcelo Bertalan Quintanilha
AU - Batto, Jean-Michel
AU - Hansen, Torben
AU - Le Paslier, Denis
AU - Linneberg, Allan René
AU - Nielsen, H. Bjørn
AU - Pelletier, Eric
AU - Renault, Pierre
AU - Sicheritz-Ponten, Thomas
AU - Turner, Keith
AU - Zhu, Hongmei
AU - Yu, Chang
AU - Li, Shengting
AU - Jian, Min
AU - Zhou, Yan
AU - Li, Yingrui
AU - Zhang, Xiuqing
AU - Li, Songgang
AU - Qin, Nan
AU - Yang, Huanming
AU - Wang, Jian
AU - Brunak, Søren
AU - Doré, Joel
AU - Guarner, Francisco
AU - Kristiansen, Karsten
AU - Pedersen, Oluf Borbye
AU - Parkhill, Julian
AU - MetaHIT Consortium
AU - Wang, Jun
N1 - Keywords: Adult; Bacteria; Cohort Studies; Contig Mapping; Denmark; Feces; Gastrointestinal Tract; Genes, Bacterial; Genes, Essential; Genome, Bacterial; Genomics; Health; Humans; Inflammatory Bowel Diseases; Metagenome; Obesity; Open Reading Frames; Overweight; Sequence Analysis, DNA; Spain
PY - 2010
Y1 - 2010
N2 - To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.
AB - To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.
U2 - 10.1038/nature08821
DO - 10.1038/nature08821
M3 - Journal article
C2 - 20203603
VL - 464
SP - 59
EP - 65
JO - Nature
JF - Nature
SN - 0028-0836
IS - 7285
ER -