A murine model for the study of mycotic mastitis

F. A. Guhad, H. E. Jensen, B. Aalbk, A. Rycroft, J. Hau*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

13 Citations (Scopus)

Abstract

This study established a murine model for the study of mycotic mastitis. The mammary glands of BALB/c mice were inoculated on the fifth day of lactation with graded doses (104, 105 and 106 cells) of a pathogenic strain of Candida krusei isolated from bovine mastitis. The animals were killed 1, 2, 3, 4 or 5 days after inoculation. In the infected mammary glands, the pathological reaction consisted of primary infiltration with heterophils and mononuclear cells, focal necrosis, formation of microabscesses, epithelial hyperplasia and some fibrosis. The severity of the changes was dose-dependent and increased with time after infection. An increase in the plasma concentrations of complement factors C1, C3c, C4 and C5, factor B and alpha-2-macroglobulin suggested that an acute phase response and activation of the complement system had occurred as a result of the infection.

Original languageEnglish
JournalJournal of Comparative Pathology
Volume113
Issue number4
Pages (from-to)315-325
Number of pages11
ISSN0021-9975
DOIs
Publication statusPublished - Nov 1995

Bibliographical note

Funding Information:
We are grateful for the support of the Danish Pasteur Association. Lise Bollen and Michael Ibuoye are acknowledged for excellent technical assistance.

Cite this