A neocentromere on human chromosome 3 without detectable alpha-satellite DNA forms morphologically normal kinetochores

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Abstract

A neocentromere at 3q26 was observed in a father and his daughter on a chromosome 3 with deleted centromeric region. No alpha-satellite DNA was detectable at the 3q26 neocentromere, but it was weakly positive with anticentromere (CREST) antibodies. Electron microscopy showed that the neocentromere formed microtubule-associated kinetochores with normal morphology and of the same size as the kinetochores of other large chromosomes. The deleted centromere formed a small linear marker chromosome that reacted strongly with anticentromere antibodies, but showed reduced kinetochore size. The 3q26 neokinetochore was stable under adverse growth conditions, which often caused kinetochore loss in the original 3-centromere on the small marker.
Original languageEnglish
JournalChromosoma
Volume107
Issue number6-7
Pages (from-to)359-65
Number of pages6
ISSN0009-5915
Publication statusPublished - 1998

Bibliographical note

Keywords: Adult; Animals; Centromere; Child, Preschool; Chromosome Deletion; Chromosomes, Human, Pair 3; DNA, Satellite; Female; Genetic Markers; Humans; Infant, Newborn; Kinetochores

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