A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life

Sheng-Yi Wu; Yurong Wen; Nelson B C Serre; Cathrine Charlotte Heiede Laursen; Andrea Grostøl Dietz; Brian R Taylor; Mikhail Drobizhev; Rosana S Molina; Abhi Aggarwal; Vladimir Rancic; Michael Becker; Klaus Ballanyi; Kaspar Podgorski; Hajime Hirase; Maiken Nedergaard; Matyáš Fendrych; M Joanne Lemieux; Daniel F Eberl; Alan R Kay; Robert E Campbell; Yi Shen

doi:10.1371/journal.pbio.3001772

A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life

Sheng-Yi Wu, Yurong Wen, Nelson B C Serre, Cathrine Charlotte Heiede Laursen, Andrea Grostøl Dietz, Brian R Taylor, Mikhail Drobizhev, Rosana S Molina, Abhi Aggarwal, Vladimir Rancic, Michael Becker, Klaus Ballanyi, Kaspar Podgorski, Hajime Hirase, Maiken Nedergaard, Matyáš Fendrych, M Joanne Lemieux, Daniel F Eberl, Alan R Kay, Robert E CampbellYi Shen

Center for Translational Neuromedicine

Research output: Contribution to journal › Journal article › Research › peer-review

13 Citations (Scopus)

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Abstract

Potassium ion (K+) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically-encoded fluorescent K+ biosensor, GINKO1, in the K+-bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K+ dynamics in multiple model organisms, including bacteria, plants, and mice.

Original language	English
Article number	e3001772
Journal	PLOS Biology
Volume	20
Issue number	9
Number of pages	23
ISSN	1544-9173
DOIs	https://doi.org/10.1371/journal.pbio.3001772
Publication status	Published - 2023

Keywords

Animals
Biosensing Techniques/methods
Fluorescence Resonance Energy Transfer/methods
Ions
Mice
Potassium

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Wu, S-Y., Wen, Y., Serre, N. B. C., Laursen, C. C. H., Dietz, A. G., Taylor, B. R., Drobizhev, M., Molina, R. S., Aggarwal, A., Rancic, V., Becker, M., Ballanyi, K., Podgorski, K., Hirase, H., Nedergaard, M., Fendrych, M., Lemieux, M. J., Eberl, D. F., Kay, A. R., ... Shen, Y. (2023). A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life. PLOS Biology, 20(9), [e3001772]. https://doi.org/10.1371/journal.pbio.3001772

A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life. / Wu, Sheng-Yi; Wen, Yurong; Serre, Nelson B C; Laursen, Cathrine Charlotte Heiede; Dietz, Andrea Grostøl; Taylor, Brian R; Drobizhev, Mikhail; Molina, Rosana S; Aggarwal, Abhi; Rancic, Vladimir; Becker, Michael; Ballanyi, Klaus; Podgorski, Kaspar; Hirase, Hajime ; Nedergaard, Maiken; Fendrych, Matyáš; Lemieux, M Joanne; Eberl, Daniel F; Kay, Alan R; Campbell, Robert E; Shen, Yi.

In: PLOS Biology, Vol. 20, No. 9, e3001772, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Wu, S-Y, Wen, Y, Serre, NBC, Laursen, CCH, Dietz, AG, Taylor, BR, Drobizhev, M, Molina, RS, Aggarwal, A, Rancic, V, Becker, M, Ballanyi, K, Podgorski, K, Hirase, H , Nedergaard, M, Fendrych, M, Lemieux, MJ, Eberl, DF, Kay, AR, Campbell, RE & Shen, Y 2023, 'A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life', PLOS Biology, vol. 20, no. 9, e3001772. https://doi.org/10.1371/journal.pbio.3001772

Wu, Sheng-Yi ; Wen, Yurong ; Serre, Nelson B C ; Laursen, Cathrine Charlotte Heiede ; Dietz, Andrea Grostøl ; Taylor, Brian R ; Drobizhev, Mikhail ; Molina, Rosana S ; Aggarwal, Abhi ; Rancic, Vladimir ; Becker, Michael ; Ballanyi, Klaus ; Podgorski, Kaspar ; Hirase, Hajime ; Nedergaard, Maiken ; Fendrych, Matyáš ; Lemieux, M Joanne ; Eberl, Daniel F ; Kay, Alan R ; Campbell, Robert E ; Shen, Yi. / A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life. In: PLOS Biology. 2023 ; Vol. 20, No. 9.

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