A vaccine displaying a trimeric influenza-A HA stem protein on capsid-like particles elicits potent and long-lasting protection in mice

Susan Thrane; Kara-Lee Aves; Ida E M Uddbäck; Christoph M Janitzek; Julianna Han; Yuhe R Yang; Andrew B Ward; Thor G Theander; Morten A Nielsen; Ali Salanti; Allan R Thomsen; Jan P Christensen; Adam F Sander

doi:10.3390/vaccines8030389

A vaccine displaying a trimeric influenza-A HA stem protein on capsid-like particles elicits potent and long-lasting protection in mice

Susan Thrane, Kara-Lee Aves, Ida E M Uddbäck, Christoph M Janitzek, Julianna Han, Yuhe R Yang, Andrew B Ward, Thor G Theander, Morten A Nielsen, Ali Salanti, Allan R Thomsen, Jan P Christensen, Adam F Sander

Research output: Contribution to journal › Journal article › Research › peer-review

17 Citations (Scopus)

102 Downloads (Pure)

Abstract

Due to constant antigenic drift and shift, current influenza-A vaccines need to be redesigned and administered annually. A universal flu vaccine (UFV) that provides long-lasting protection against both seasonal and emerging pandemic influenza strains is thus urgently needed. The hemagglutinin (HA) stem antigen is a promising target for such a vaccine as it contains neutralizing epitopes, known to induce cross-protective IgG responses against a wide variety of influenza subtypes. In this study, we describe the development of a UFV candidate consisting of a HAstem trimer displayed on the surface of rigid capsid-like particles (CLP). Compared to soluble unconjugated HAstem trimer, the CLP-HAstem particles induced a more potent, long-lasting immune response and were able to protect mice against both homologous and heterologous H1N1 influenza challenge, even after a single dose.

Original language	English
Article number	389
Journal	Vaccines
Volume	8
Issue number	3
ISSN	2076-393X
DOIs	https://doi.org/10.3390/vaccines8030389
Publication status	Published - 2020

Access to Document

10.3390/vaccines8030389Licence: CC BY

A Vaccine Displaying a Trimeric Influenza-A HA Stem Protein on Capsid-Like Particles Elicits Potent and Long-Lasting Protection in MiceFinal published version, 5.06 MBLicence: CC BY

Cite this

Thrane, S., Aves, K-L., Uddbäck, I. E. M., Janitzek, C. M., Han, J., Yang, Y. R., Ward, A. B., Theander, T. G., Nielsen, M. A., Salanti, A., Thomsen, A. R., Christensen, J. P., & Sander, A. F. (2020). A vaccine displaying a trimeric influenza-A HA stem protein on capsid-like particles elicits potent and long-lasting protection in mice. Vaccines, 8(3), [389]. https://doi.org/10.3390/vaccines8030389

A vaccine displaying a trimeric influenza-A HA stem protein on capsid-like particles elicits potent and long-lasting protection in mice. / Thrane, Susan; Aves, Kara-Lee ; Uddbäck, Ida E M; Janitzek, Christoph M; Han, Julianna; Yang, Yuhe R; Ward, Andrew B; Theander, Thor G ; Nielsen, Morten A ; Salanti, Ali ; Thomsen, Allan R ; Christensen, Jan P ; Sander, Adam F.

In: Vaccines, Vol. 8, No. 3, 389, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Thrane, S, Aves, K-L , Uddbäck, IEM, Janitzek, CM, Han, J, Yang, YR, Ward, AB, Theander, TG , Nielsen, MA , Salanti, A , Thomsen, AR , Christensen, JP & Sander, AF 2020, 'A vaccine displaying a trimeric influenza-A HA stem protein on capsid-like particles elicits potent and long-lasting protection in mice', Vaccines, vol. 8, no. 3, 389. https://doi.org/10.3390/vaccines8030389

Thrane, Susan ; Aves, Kara-Lee ; Uddbäck, Ida E M ; Janitzek, Christoph M ; Han, Julianna ; Yang, Yuhe R ; Ward, Andrew B ; Theander, Thor G ; Nielsen, Morten A ; Salanti, Ali ; Thomsen, Allan R ; Christensen, Jan P ; Sander, Adam F. / A vaccine displaying a trimeric influenza-A HA stem protein on capsid-like particles elicits potent and long-lasting protection in mice. In: Vaccines. 2020 ; Vol. 8, No. 3.

@article{4d0318861db94f5c8feb65aceb08d056,

title = "A vaccine displaying a trimeric influenza-A HA stem protein on capsid-like particles elicits potent and long-lasting protection in mice",

abstract = "Due to constant antigenic drift and shift, current influenza-A vaccines need to be redesigned and administered annually. A universal flu vaccine (UFV) that provides long-lasting protection against both seasonal and emerging pandemic influenza strains is thus urgently needed. The hemagglutinin (HA) stem antigen is a promising target for such a vaccine as it contains neutralizing epitopes, known to induce cross-protective IgG responses against a wide variety of influenza subtypes. In this study, we describe the development of a UFV candidate consisting of a HAstem trimer displayed on the surface of rigid capsid-like particles (CLP). Compared to soluble unconjugated HAstem trimer, the CLP-HAstem particles induced a more potent, long-lasting immune response and were able to protect mice against both homologous and heterologous H1N1 influenza challenge, even after a single dose.",

author = "Susan Thrane and Kara-Lee Aves and Uddb{\"a}ck, {Ida E M} and Janitzek, {Christoph M} and Julianna Han and Yang, {Yuhe R} and Ward, {Andrew B} and Theander, {Thor G} and Nielsen, {Morten A} and Ali Salanti and Thomsen, {Allan R} and Christensen, {Jan P} and Sander, {Adam F}",

year = "2020",

doi = "10.3390/vaccines8030389",

language = "English",

volume = "8",

journal = "Vaccines",

issn = "2076-393X",

publisher = "MDPI AG",

number = "3",

}

TY - JOUR

T1 - A vaccine displaying a trimeric influenza-A HA stem protein on capsid-like particles elicits potent and long-lasting protection in mice

AU - Thrane, Susan

AU - Aves, Kara-Lee

AU - Uddbäck, Ida E M

AU - Janitzek, Christoph M

AU - Han, Julianna

AU - Yang, Yuhe R

AU - Ward, Andrew B

AU - Theander, Thor G

AU - Nielsen, Morten A

AU - Salanti, Ali

AU - Thomsen, Allan R

AU - Christensen, Jan P

AU - Sander, Adam F

PY - 2020

Y1 - 2020

N2 - Due to constant antigenic drift and shift, current influenza-A vaccines need to be redesigned and administered annually. A universal flu vaccine (UFV) that provides long-lasting protection against both seasonal and emerging pandemic influenza strains is thus urgently needed. The hemagglutinin (HA) stem antigen is a promising target for such a vaccine as it contains neutralizing epitopes, known to induce cross-protective IgG responses against a wide variety of influenza subtypes. In this study, we describe the development of a UFV candidate consisting of a HAstem trimer displayed on the surface of rigid capsid-like particles (CLP). Compared to soluble unconjugated HAstem trimer, the CLP-HAstem particles induced a more potent, long-lasting immune response and were able to protect mice against both homologous and heterologous H1N1 influenza challenge, even after a single dose.

AB - Due to constant antigenic drift and shift, current influenza-A vaccines need to be redesigned and administered annually. A universal flu vaccine (UFV) that provides long-lasting protection against both seasonal and emerging pandemic influenza strains is thus urgently needed. The hemagglutinin (HA) stem antigen is a promising target for such a vaccine as it contains neutralizing epitopes, known to induce cross-protective IgG responses against a wide variety of influenza subtypes. In this study, we describe the development of a UFV candidate consisting of a HAstem trimer displayed on the surface of rigid capsid-like particles (CLP). Compared to soluble unconjugated HAstem trimer, the CLP-HAstem particles induced a more potent, long-lasting immune response and were able to protect mice against both homologous and heterologous H1N1 influenza challenge, even after a single dose.

U2 - 10.3390/vaccines8030389

DO - 10.3390/vaccines8030389

M3 - Journal article

C2 - 32679905

VL - 8

JO - Vaccines

JF - Vaccines

SN - 2076-393X

IS - 3

M1 - 389

ER -