ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importers

Yuqin Zhang; Himabindu Vasuki Kilambi; Jie Liu; Hamutal Bar; Shani Lazary; Aiman Egbaria; Dagmar Ripper; Laurence Charrier; Zeinu Mussa Belew; Nikolai Wulff; Suresh Damodaran; Hussam Hassan Nour-Eldin; Asaph Aharoni; Laura Ragni; Lucia Strader; Nir Sade; Roy Weinstain; Markus Geisler; Eilon Shani

doi:10.1126/sciadv.abf6069

ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importers

Yuqin Zhang, Himabindu Vasuki Kilambi, Jie Liu, Hamutal Bar, Shani Lazary, Aiman Egbaria, Dagmar Ripper, Laurence Charrier, Zeinu Mussa Belew, Nikolai Wulff, Suresh Damodaran, Hussam Hassan Nour-Eldin, Asaph Aharoni, Laura Ragni, Lucia Strader, Nir Sade, Roy Weinstain, Markus Geisler, Eilon Shani^*

^*Corresponding author for this work

Section for Molecular Plant Biology

Research output: Contribution to journal › Journal article › Research › peer-review

44 Citations (Scopus)

22 Downloads (Pure)

Abstract

The effects of abscisic acid (ABA) on plant growth, development, and response to the environment depend on local ABA concentrations. Here, we show that in Arabidopsis, ABA homeostasis is regulated by two previously unknown ABA transporters. Adenosine triphosphate-binding cassette subfamily G member 17 (ABCG17) and ABCG18 are localized to the plasma membranes of leaf mesophyll and cortex cells to redundantly promote ABA import, leading to conjugated inactive ABA sinks, thus restricting stomatal closure. ABCG17 and ABCG18 double knockdown revealed that the transporters encoded by these genes not only limit stomatal aperture size, conductance, and transpiration while increasing water use efficiency but also control ABA translocation from the shoot to the root to regulate lateral root emergence. Under abiotic stress conditions, ABCG17 and ABCG18 are transcriptionally repressed, promoting active ABA movement and response. The transport mechanism mediated by ABCG17 and ABCG18 allows plants to maintain ABA homeostasis under normal growth conditions.

Original language	English
Article number	eabf6069
Journal	Science Advances
Volume	7
Issue number	43
Number of pages	18
ISSN	2375-2548
DOIs	https://doi.org/10.1126/sciadv.abf6069
Publication status	Published - 2021

Bibliographical note

Publisher Copyright:
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.

Access to Document

10.1126/sciadv.abf6069Licence: CC BY-NC

ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importersFinal published version, 8.86 MBLicence: CC BY-NC

Cite this

Zhang, Y., Kilambi, H. V., Liu, J., Bar, H., Lazary, S., Egbaria, A., Ripper, D., Charrier, L., Belew, Z. M., Wulff, N., Damodaran, S., Nour-Eldin, H. H., Aharoni, A., Ragni, L., Strader, L., Sade, N., Weinstain, R., Geisler, M., & Shani, E. (2021). ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importers. Science Advances, 7(43), [eabf6069]. https://doi.org/10.1126/sciadv.abf6069

ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importers. / Zhang, Yuqin; Kilambi, Himabindu Vasuki; Liu, Jie; Bar, Hamutal; Lazary, Shani; Egbaria, Aiman; Ripper, Dagmar; Charrier, Laurence; Belew, Zeinu Mussa; Wulff, Nikolai; Damodaran, Suresh; Nour-Eldin, Hussam Hassan; Aharoni, Asaph; Ragni, Laura; Strader, Lucia; Sade, Nir; Weinstain, Roy; Geisler, Markus; Shani, Eilon.

In: Science Advances, Vol. 7, No. 43, eabf6069, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Zhang, Y, Kilambi, HV, Liu, J, Bar, H, Lazary, S, Egbaria, A, Ripper, D, Charrier, L, Belew, ZM, Wulff, N, Damodaran, S, Nour-Eldin, HH, Aharoni, A, Ragni, L, Strader, L, Sade, N, Weinstain, R, Geisler, M & Shani, E 2021, 'ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importers', Science Advances, vol. 7, no. 43, eabf6069. https://doi.org/10.1126/sciadv.abf6069

Zhang, Yuqin ; Kilambi, Himabindu Vasuki ; Liu, Jie ; Bar, Hamutal ; Lazary, Shani ; Egbaria, Aiman ; Ripper, Dagmar ; Charrier, Laurence ; Belew, Zeinu Mussa ; Wulff, Nikolai ; Damodaran, Suresh ; Nour-Eldin, Hussam Hassan ; Aharoni, Asaph ; Ragni, Laura ; Strader, Lucia ; Sade, Nir ; Weinstain, Roy ; Geisler, Markus ; Shani, Eilon. / ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importers. In: Science Advances. 2021 ; Vol. 7, No. 43.

@article{e1d2e65291d24035996f2cd848a2df7c,

title = "ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importers",

abstract = "The effects of abscisic acid (ABA) on plant growth, development, and response to the environment depend on local ABA concentrations. Here, we show that in Arabidopsis, ABA homeostasis is regulated by two previously unknown ABA transporters. Adenosine triphosphate-binding cassette subfamily G member 17 (ABCG17) and ABCG18 are localized to the plasma membranes of leaf mesophyll and cortex cells to redundantly promote ABA import, leading to conjugated inactive ABA sinks, thus restricting stomatal closure. ABCG17 and ABCG18 double knockdown revealed that the transporters encoded by these genes not only limit stomatal aperture size, conductance, and transpiration while increasing water use efficiency but also control ABA translocation from the shoot to the root to regulate lateral root emergence. Under abiotic stress conditions, ABCG17 and ABCG18 are transcriptionally repressed, promoting active ABA movement and response. The transport mechanism mediated by ABCG17 and ABCG18 allows plants to maintain ABA homeostasis under normal growth conditions. ",

author = "Yuqin Zhang and Kilambi, {Himabindu Vasuki} and Jie Liu and Hamutal Bar and Shani Lazary and Aiman Egbaria and Dagmar Ripper and Laurence Charrier and Belew, {Zeinu Mussa} and Nikolai Wulff and Suresh Damodaran and Nour-Eldin, {Hussam Hassan} and Asaph Aharoni and Laura Ragni and Lucia Strader and Nir Sade and Roy Weinstain and Markus Geisler and Eilon Shani",

note = "Publisher Copyright: Copyright {\textcopyright} 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.",

year = "2021",

doi = "10.1126/sciadv.abf6069",

language = "English",

volume = "7",

journal = "Science advances",

issn = "2375-2548",

publisher = "American Association for the Advancement of Science",

number = "43",

}

TY - JOUR

T1 - ABA homeostasis and long-distance translocation are redundantly regulated by ABCG ABA importers

AU - Zhang, Yuqin

AU - Kilambi, Himabindu Vasuki

AU - Liu, Jie

AU - Bar, Hamutal

AU - Lazary, Shani

AU - Egbaria, Aiman

AU - Ripper, Dagmar

AU - Charrier, Laurence

AU - Belew, Zeinu Mussa

AU - Wulff, Nikolai

AU - Damodaran, Suresh

AU - Nour-Eldin, Hussam Hassan

AU - Aharoni, Asaph

AU - Ragni, Laura

AU - Strader, Lucia

AU - Sade, Nir

AU - Weinstain, Roy

AU - Geisler, Markus

AU - Shani, Eilon

PY - 2021

Y1 - 2021

N2 - The effects of abscisic acid (ABA) on plant growth, development, and response to the environment depend on local ABA concentrations. Here, we show that in Arabidopsis, ABA homeostasis is regulated by two previously unknown ABA transporters. Adenosine triphosphate-binding cassette subfamily G member 17 (ABCG17) and ABCG18 are localized to the plasma membranes of leaf mesophyll and cortex cells to redundantly promote ABA import, leading to conjugated inactive ABA sinks, thus restricting stomatal closure. ABCG17 and ABCG18 double knockdown revealed that the transporters encoded by these genes not only limit stomatal aperture size, conductance, and transpiration while increasing water use efficiency but also control ABA translocation from the shoot to the root to regulate lateral root emergence. Under abiotic stress conditions, ABCG17 and ABCG18 are transcriptionally repressed, promoting active ABA movement and response. The transport mechanism mediated by ABCG17 and ABCG18 allows plants to maintain ABA homeostasis under normal growth conditions.

AB - The effects of abscisic acid (ABA) on plant growth, development, and response to the environment depend on local ABA concentrations. Here, we show that in Arabidopsis, ABA homeostasis is regulated by two previously unknown ABA transporters. Adenosine triphosphate-binding cassette subfamily G member 17 (ABCG17) and ABCG18 are localized to the plasma membranes of leaf mesophyll and cortex cells to redundantly promote ABA import, leading to conjugated inactive ABA sinks, thus restricting stomatal closure. ABCG17 and ABCG18 double knockdown revealed that the transporters encoded by these genes not only limit stomatal aperture size, conductance, and transpiration while increasing water use efficiency but also control ABA translocation from the shoot to the root to regulate lateral root emergence. Under abiotic stress conditions, ABCG17 and ABCG18 are transcriptionally repressed, promoting active ABA movement and response. The transport mechanism mediated by ABCG17 and ABCG18 allows plants to maintain ABA homeostasis under normal growth conditions.

U2 - 10.1126/sciadv.abf6069

DO - 10.1126/sciadv.abf6069

M3 - Journal article

C2 - 34669479

AN - SCOPUS:85117703648

VL - 7

JO - Science advances

JF - Science advances

SN - 2375-2548

IS - 43

M1 - eabf6069

ER -