Abstract
Drug release from a lipid-based drug delivery system (LbDDS) is typically studied in vitro using a one-step intestinal digestion model. However, lately the importance of incorporating gastric digestion has been stressed. The aim of the present study was to compare a two-step gastro-intestinal (GI) in vitro digestion model to the commonly used one-step intestinal digestion model. The models were evaluated by studying release of the model drug A1260 from two LbDDSs (F-I and F-II), for which in vivo pharmacokinetic data from oral administration to beagle dogs were available. The amount of A1260 recovered in the aqueous phases during and after the GI digestion of F-I and F-II was related to the Cmax and AUC0- 48h of the plasma concentration-time profiles of each formulation and produced a rank order in vitroin vivo (IVIV) relation. In comparison, a similar IVIV rank ordering was obtained when relating the amount of A1260 recovered in the aqueous phase prior (t = 0 min), and following 15 min of intestinal digestion, to the plasma concentration-time profiles. However, after 60 min of intestinal digestion, the LbDDSs performed equally in the one-step in vitro digestion model, contrary to what was observed in the two-step digestion model, and in vivo. As the GI digestion model produced a clearer distinction in terms of LbDDS rank ordering of the two LbDDSs, compared to the intestinal digestion model, it was found to be a promising in vitro model to study and estimate the LbDDS behavior in vivo.
Original language | English |
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Journal | Molecular Pharmaceutics |
Volume | 17 |
Issue number | 9 |
Pages (from-to) | 3214-3222 |
Number of pages | 9 |
ISSN | 1543-8384 |
DOIs | |
Publication status | Published - 2020 |
Keywords
- gastro-intestinal digestion
- in vitro
- in vivo
- lipid-based drug delivery system
- oral biopharmaceutical tools (OrBiTo)
- WATER-SOLUBLE DRUGS
- LIPOLYSIS MODEL
- GASTROINTESTINAL LIPOLYSIS
- VIVO PERFORMANCE
- FORMULATIONS
- LIPASES
- TESTS
- BIOAVAILABILITY
- ESTABLISHMENT
- DISSOLUTION