Akt and 14-3-3eta regulate Miz1 to control cell-cycle arrest after DNA damage.

Michael Wanzel; Daniela Kleine-Kohlbrecher; Steffi Herold; Andreas Hock; Katrien Berns; Jongsun Park; Brian Hemmings; Martin Eilers

doi:10.1038/ncb1202

Akt and 14-3-3eta regulate Miz1 to control cell-cycle arrest after DNA damage.

Michael Wanzel, Daniela Kleine-Kohlbrecher, Steffi Herold, Andreas Hock, Katrien Berns, Jongsun Park, Brian Hemmings, Martin Eilers

Research output: Contribution to journal › Journal article › Research › peer-review

76 Citations (Scopus)

Abstract

The transcription factor Miz1 is required for DNA-damage-induced cell-cycle arrest. We have now identified 14-3-3eta as a gene that inhibits Miz1 function through interaction with its DNA binding domain. Binding of 14-3-3eta to Miz1 depends on phosphorylation by Akt and regulates the recovery of cells from arrest after DNA damage. Miz1 has two functions in response to DNA damage: first, it is required for upregulation of a large group of genes, a function that is regulated by c-Myc, but not by 14-3-3eta; second, Miz1 represses the expression of many genes in response to DNA damage in an Akt- and 14-3-3eta-regulated manner.

Original language	English
Journal	Nature Cell Biology
Volume	7
Issue number	1
Pages (from-to)	30-41
Number of pages	11
ISSN	1465-7392
DOIs	https://doi.org/10.1038/ncb1202
Publication status	Published - 2004
Externally published	Yes

Bibliographical note

Keywords: 14-3-3 Proteins; Animals; Cell Cycle; Cell Cycle Proteins; DNA Damage; DNA-Binding Proteins; Gene Expression Regulation; Gene Library; Hela Cells; Humans; Kruppel-Like Transcription Factors; Mice; Molecular Sequence Data; NIH 3T3 Cells; Phosphorylation; Protein Binding; Protein Structure, Tertiary; Protein-Serine-Threonine Kinases; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-akt; Proto-Oncogene Proteins c-myc; Rats; Transcription Factors; Up-Regulation

Access to Document

10.1038/ncb1202

Cite this

@article{e269f46054a311dd8d9f000ea68e967b,

title = "Akt and 14-3-3eta regulate Miz1 to control cell-cycle arrest after DNA damage.",

abstract = "The transcription factor Miz1 is required for DNA-damage-induced cell-cycle arrest. We have now identified 14-3-3eta as a gene that inhibits Miz1 function through interaction with its DNA binding domain. Binding of 14-3-3eta to Miz1 depends on phosphorylation by Akt and regulates the recovery of cells from arrest after DNA damage. Miz1 has two functions in response to DNA damage: first, it is required for upregulation of a large group of genes, a function that is regulated by c-Myc, but not by 14-3-3eta; second, Miz1 represses the expression of many genes in response to DNA damage in an Akt- and 14-3-3eta-regulated manner.",

author = "Michael Wanzel and Daniela Kleine-Kohlbrecher and Steffi Herold and Andreas Hock and Katrien Berns and Jongsun Park and Brian Hemmings and Martin Eilers",

note = "Keywords: 14-3-3 Proteins; Animals; Cell Cycle; Cell Cycle Proteins; DNA Damage; DNA-Binding Proteins; Gene Expression Regulation; Gene Library; Hela Cells; Humans; Kruppel-Like Transcription Factors; Mice; Molecular Sequence Data; NIH 3T3 Cells; Phosphorylation; Protein Binding; Protein Structure, Tertiary; Protein-Serine-Threonine Kinases; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-akt; Proto-Oncogene Proteins c-myc; Rats; Transcription Factors; Up-Regulation",

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doi = "10.1038/ncb1202",

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volume = "7",

pages = "30--41",

journal = "Nature Cell Biology",

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T1 - Akt and 14-3-3eta regulate Miz1 to control cell-cycle arrest after DNA damage.

AU - Wanzel, Michael

AU - Kleine-Kohlbrecher, Daniela

AU - Herold, Steffi

AU - Hock, Andreas

AU - Berns, Katrien

AU - Park, Jongsun

AU - Hemmings, Brian

AU - Eilers, Martin

N1 - Keywords: 14-3-3 Proteins; Animals; Cell Cycle; Cell Cycle Proteins; DNA Damage; DNA-Binding Proteins; Gene Expression Regulation; Gene Library; Hela Cells; Humans; Kruppel-Like Transcription Factors; Mice; Molecular Sequence Data; NIH 3T3 Cells; Phosphorylation; Protein Binding; Protein Structure, Tertiary; Protein-Serine-Threonine Kinases; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-akt; Proto-Oncogene Proteins c-myc; Rats; Transcription Factors; Up-Regulation

PY - 2004

Y1 - 2004

N2 - The transcription factor Miz1 is required for DNA-damage-induced cell-cycle arrest. We have now identified 14-3-3eta as a gene that inhibits Miz1 function through interaction with its DNA binding domain. Binding of 14-3-3eta to Miz1 depends on phosphorylation by Akt and regulates the recovery of cells from arrest after DNA damage. Miz1 has two functions in response to DNA damage: first, it is required for upregulation of a large group of genes, a function that is regulated by c-Myc, but not by 14-3-3eta; second, Miz1 represses the expression of many genes in response to DNA damage in an Akt- and 14-3-3eta-regulated manner.

AB - The transcription factor Miz1 is required for DNA-damage-induced cell-cycle arrest. We have now identified 14-3-3eta as a gene that inhibits Miz1 function through interaction with its DNA binding domain. Binding of 14-3-3eta to Miz1 depends on phosphorylation by Akt and regulates the recovery of cells from arrest after DNA damage. Miz1 has two functions in response to DNA damage: first, it is required for upregulation of a large group of genes, a function that is regulated by c-Myc, but not by 14-3-3eta; second, Miz1 represses the expression of many genes in response to DNA damage in an Akt- and 14-3-3eta-regulated manner.

U2 - 10.1038/ncb1202

DO - 10.1038/ncb1202

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