Alpha-adrenergic receptors in rat skeletal muscle

S Rattigan, G J Appleby, S J Edwards, W J McKinstry, E Q Colquhoun, M G Clark, Erik A. Richter

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    Abstract

    Sarcolemma-enriched preparations from muscles rich in slow oxidative red fibres contained specific binding sites for the alpha 1 antagonist, prazosin (e.g. soleus Kd 0.13 nM, Bmax 29 fmol/mg protein). Binding sites for prazosin were almost absent from white muscle. Displacement of prazosin binding from sarcolemma of soleus muscle (phentolamine greater than phenylephrine greater than idazoxan greater than yohimbine) suggested that the receptors were alpha 1. Binding sites for dihydroalprenolol (beta antagonist) were also more concentrated on red than white muscle and outnumbered prazosin sites by approx. 10:1. Binding sites for idazoxan (alpha 2 antagonist) were undetectable. Contamination of sarcolemma-enriched preparations by endothelial tissue indicated by the activity of angiotensin converting enzyme did not correlate with prazosin binding. It is concluded that post-synaptic alpha 1 adrenergic receptors are present on the sarcolemma of slow oxidative red fibres of rat skeletal muscle. The presence provides the mechanistic basis for apparent alpha-adrenergic effects to increase glucose and oxygen uptake in perfused rat hindquarter.

    Original languageEnglish
    JournalBiochemical and Biophysical Research Communications
    Volume136
    Issue number3
    Pages (from-to)1071-1077
    Number of pages7
    ISSN0006-291X
    Publication statusPublished - 1986

    Keywords

    • Animals
    • Binding Sites
    • Dihydroalprenolol
    • Dioxanes
    • Female
    • Idazoxan
    • Kinetics
    • Male
    • Muscles
    • Peptidyl-Dipeptidase A
    • Phentolamine
    • Phenylephrine
    • Prazosin
    • Rats
    • Receptors, Adrenergic, alpha
    • Sarcolemma
    • Yohimbine

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