An online resource for GPCR structure determination and analysis

Christian Munk; Eshita Mutt; Vignir Isberg; Louise F Nikolajsen; Janne M Bibbe; Tilman Flock; Michael A Hanson; Raymond C Stevens; Xavier Deupi; David E Gloriam

doi:10.1038/s41592-018-0302-x

An online resource for GPCR structure determination and analysis

Christian Munk, Eshita Mutt, Vignir Isberg, Louise F Nikolajsen, Janne M Bibbe, Tilman Flock, Michael A Hanson, Raymond C Stevens, Xavier Deupi, David E Gloriam

Biostructural Research

Research output: Contribution to journal › Review › Research › peer-review

104 Citations (Scopus)

Abstract

G-protein-coupled receptors (GPCRs) transduce physiological and sensory stimuli into appropriate cellular responses and mediate the actions of one-third of drugs. GPCR structural studies have revealed the general bases of receptor activation, signaling, drug action and allosteric modulation, but so far cover only 13% of nonolfactory receptors. We broadly surveyed the receptor modifications/engineering and methods used to produce all available GPCR crystal and cryo-electron microscopy (cryo-EM) structures, and present an interactive resource integrated in GPCRdb ( http://www.gpcrdb.org ) to assist users in designing constructs and browsing appropriate experimental conditions for structure studies.

Original language	English
Journal	Nature Methods
Volume	16
Issue number	2
Pages (from-to)	151-162
ISSN	1548-7091
DOIs	https://doi.org/10.1038/s41592-018-0302-x
Publication status	Published - 2019

Keywords

Allosteric Site
Animals
Cattle
Computational Biology/methods
Cryoelectron Microscopy
Crystallography, X-Ray
Databases, Protein
Drug Design
Glycosylation
HEK293 Cells
Humans
Internet
Mutation
Phosphorylation
Protein Domains
Protein Engineering
Receptors, G-Protein-Coupled/genetics
Rhodopsin/chemistry
Signal Transduction
Software

Access to Document

10.1038/s41592-018-0302-x

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881186/pdf/EMS84985.pdfLicence: Other

Cite this

An online resource for GPCR structure determination and analysis. / Munk, Christian; Mutt, Eshita; Isberg, Vignir; Nikolajsen, Louise F; Bibbe, Janne M; Flock, Tilman; Hanson, Michael A; Stevens, Raymond C; Deupi, Xavier; Gloriam, David E.

In: Nature Methods, Vol. 16, No. 2, 2019, p. 151-162.

Research output: Contribution to journal › Review › Research › peer-review

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AU - Flock, Tilman

AU - Hanson, Michael A

AU - Stevens, Raymond C

AU - Deupi, Xavier

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