An online resource for GPCR structure determination and analysis

Christian Munk, Eshita Mutt, Vignir Isberg, Louise F Nikolajsen, Janne M Bibbe, Tilman Flock, Michael A Hanson, Raymond C Stevens, Xavier Deupi, David E Gloriam

Research output: Contribution to journalReviewResearchpeer-review

104 Citations (Scopus)

Abstract

G-protein-coupled receptors (GPCRs) transduce physiological and sensory stimuli into appropriate cellular responses and mediate the actions of one-third of drugs. GPCR structural studies have revealed the general bases of receptor activation, signaling, drug action and allosteric modulation, but so far cover only 13% of nonolfactory receptors. We broadly surveyed the receptor modifications/engineering and methods used to produce all available GPCR crystal and cryo-electron microscopy (cryo-EM) structures, and present an interactive resource integrated in GPCRdb ( http://www.gpcrdb.org ) to assist users in designing constructs and browsing appropriate experimental conditions for structure studies.

Original languageEnglish
JournalNature Methods
Volume16
Issue number2
Pages (from-to)151-162
ISSN1548-7091
DOIs
Publication statusPublished - 2019

Keywords

  • Allosteric Site
  • Animals
  • Cattle
  • Computational Biology/methods
  • Cryoelectron Microscopy
  • Crystallography, X-Ray
  • Databases, Protein
  • Drug Design
  • Glycosylation
  • HEK293 Cells
  • Humans
  • Internet
  • Mutation
  • Phosphorylation
  • Protein Domains
  • Protein Engineering
  • Receptors, G-Protein-Coupled/genetics
  • Rhodopsin/chemistry
  • Signal Transduction
  • Software

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