@inbook{f44e59fbbbb943f19f21901abf1458e0,
title = "Analysis by flow cytometry of α2-macroglobulin and nonimmune IgM-binding to Plasmodium falciparum-infected erythrocytes",
abstract = "Several members of the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family can bind human serum proteins such as IgM and α2-macroglobulin (α2M). This binding seems to play a role in pathogenesis and immune evasion by improving the avidity of PfEMP1-mediated binding to erythrocyte receptors and/or by masking antibody epitopes in PfEMP1. In this protocol, we describe a flow cytometry-based protocol to evaluate IgM- and α2M-binding to intact and unfixed mature-stage IEs. The method can be used for laboratory clones and field isolates.",
keywords = "Antibodies, Protozoan, Erythrocytes/metabolism, Female, Flow Cytometry, Humans, Immunoglobulin M, Malaria, Falciparum, Plasmodium falciparum/metabolism, Pregnancy, Pregnancy-Associated alpha 2-Macroglobulins/metabolism, Protozoan Proteins/metabolism",
author = "Lars Hviid and Mary Lopez-Perez",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2189-9_32",
language = "English",
isbn = "978-1-0716-2188-2",
volume = "2470",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "435--444",
booktitle = "Malaria Immunology",
address = "United States",
}