TY - JOUR
T1 - Application of Low-Frequency Raman Spectroscopy to Probe Dynamics of Lipid Mesophase Transformations upon Hydration
AU - Krog, Lasse S.
AU - Kirkensgaard, Jacob J.K.
AU - Foderà, Vito
AU - Boyd, Ben J.
AU - Be̅rziņš, Ka̅rlis
N1 - Funding Information:
The authors acknowledge the Novo Nordisk Foundation for supporting this work. Ben Boyd, Ka̅rlis Be̅rziņš, and Lasse Krog were supported by a Novo Nordisk Laureate Research Fellowship awarded to Ben Boyd.
Publisher Copyright:
© 2023 American Chemical Society
PY - 2023
Y1 - 2023
N2 - Low-frequency Raman (LFR) spectroscopy is presented as a viable tool for studying the hydration characteristics of lyotropic liquid crystal systems herein. Monoolein was used as a model compound, and its structural changes were probed both in situ and ex situ which enabled a comparison between different hydration states. A custom-built instrumental configuration allowed the advantages of LFR spectroscopy to be utilized for dynamic hydration analysis. On the other hand, static measurements of equilibrated systems (i.e., with varied aqueous content) showcased the structural sensitivity of LFR spectroscopy. The subtle differences not intuitively observed between similar self-assembled architectures were distinguished by chemometric analysis that directly correlated with the results from small-angle X-ray scattering (SAXS), which is the current “gold standard” method for determining the structure of such materials.
AB - Low-frequency Raman (LFR) spectroscopy is presented as a viable tool for studying the hydration characteristics of lyotropic liquid crystal systems herein. Monoolein was used as a model compound, and its structural changes were probed both in situ and ex situ which enabled a comparison between different hydration states. A custom-built instrumental configuration allowed the advantages of LFR spectroscopy to be utilized for dynamic hydration analysis. On the other hand, static measurements of equilibrated systems (i.e., with varied aqueous content) showcased the structural sensitivity of LFR spectroscopy. The subtle differences not intuitively observed between similar self-assembled architectures were distinguished by chemometric analysis that directly correlated with the results from small-angle X-ray scattering (SAXS), which is the current “gold standard” method for determining the structure of such materials.
U2 - 10.1021/acs.jpcb.2c08150
DO - 10.1021/acs.jpcb.2c08150
M3 - Journal article
C2 - 36999811
AN - SCOPUS:85151878617
VL - 127
SP - 3223
EP - 3230
JO - Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical
JF - Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical
SN - 1520-6106
IS - 14
ER -