Abstract
Aims/Purpose: To investigate the cell survival of human conjunctival goblet cells (CGs) when incubated with preserved and preservative-free (PF) artificial tears (AT) used against dry eye disease (DED). The following ATs were tested: 0.011% benzalkonium chloride (BAK)-preserved Viskøse® Øjendråber, 0.006% BAK-preserved Oftagel®, 0.005% BAK-preserved Oculac®, 0.001% PolyQuad™-preserved Systane® Ultra, 0.01% Cetrimide-preserved Artelac®, and PF ATs: Systane® Ultra, Oculac®, Oftagel®, and Thealoz® Duo.
Methods: Primary cultures of human conjunctival CGs were cultivated from donor conjunctiva. After incubation with the above-mentioned ATs for 2 h, CG survival was analysed using a lactate dehydrogenase assay and calculated to a negative control.
Results: AT preserved with 0.011% BAK reduced GC survival by 28% compared to negative control (p = 0.001). The remaining ATs did not significantly alter the cell survival.
Conclusions: BAK-preserved ATs cause GC death in a concentration-dependent manner. Reduction of the GC density may contribute to development of DED. PF-ATs and PolyQuad- and Cetrimide-preserved ATs may thus be a better choice for treating DED than BAK preserved ATs, as the latter may add to the ocular surface disease.
Methods: Primary cultures of human conjunctival CGs were cultivated from donor conjunctiva. After incubation with the above-mentioned ATs for 2 h, CG survival was analysed using a lactate dehydrogenase assay and calculated to a negative control.
Results: AT preserved with 0.011% BAK reduced GC survival by 28% compared to negative control (p = 0.001). The remaining ATs did not significantly alter the cell survival.
Conclusions: BAK-preserved ATs cause GC death in a concentration-dependent manner. Reduction of the GC density may contribute to development of DED. PF-ATs and PolyQuad- and Cetrimide-preserved ATs may thus be a better choice for treating DED than BAK preserved ATs, as the latter may add to the ocular surface disease.
| Original language | English |
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| Journal | Acta Ophthalmologica |
| Volume | 102 |
| Issue number | S279 |
| ISSN | 1755-375X |
| DOIs | |
| Publication status | Published - 2024 |
| Event | European Association for Vision and Eye Research - Valencia Congress Center, Valencia, Spain Duration: 26 Oct 2023 → 28 Oct 2023 |
Conference
| Conference | European Association for Vision and Eye Research |
|---|---|
| Location | Valencia Congress Center |
| Country/Territory | Spain |
| City | Valencia |
| Period | 26/10/2023 → 28/10/2023 |