ASSAYS FOR DETECTING GLUCOSIDASE ACTIVITY

Marie Bøjstrup (Inventor), Caspar Elo Christensen (Inventor), Ole Hindsgaul (Inventor)

Research output: Patent

Abstract

The present invention relates to a method of detecting α-(1→6)-glucosidase activity in a sample. The method is for example useful for determining the limit dextrinase activity in a sample. The method involves use of an oligosaccharide substrate of the formula X-(glucoside)n-*(glucoside)m-Z—Y, where X is a blocking group, -* is a α-(1→6)-glucosidic linkage and Y is a detectable label. Upon cleavage of the α-(1→6)-glucosidic linkage, the detectable label is released and thus the α-(1→6)-glucosidase activity can be determined. The invention also relates to the oligosaccharide substrate per se.
Original languageEnglish
Country/TerritoryDenmark
Publication statusPublished - 21 May 2015

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